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Mice Microbiota Composition Changes by Inulin Feeding with a Long Fasting Period under a Two-Meals-Per-Day Schedule

Water-soluble dietary fiber is known to modulate fecal microbiota. Although there are a few reports investigating the effects of fiber intake timing on metabolism, there are none on the effect of intake timing on microbiota. Therefore, in this study, we examined the timing effects of inulin-containi...

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Autores principales: Sasaki, Hiroyuki, Miyakawa, Hiroki, Watanabe, Aya, Nakayama, Yuki, Lyu, Yijin, Hama, Koki, Shibata, Shigenobu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893728/
https://www.ncbi.nlm.nih.gov/pubmed/31744168
http://dx.doi.org/10.3390/nu11112802
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author Sasaki, Hiroyuki
Miyakawa, Hiroki
Watanabe, Aya
Nakayama, Yuki
Lyu, Yijin
Hama, Koki
Shibata, Shigenobu
author_facet Sasaki, Hiroyuki
Miyakawa, Hiroki
Watanabe, Aya
Nakayama, Yuki
Lyu, Yijin
Hama, Koki
Shibata, Shigenobu
author_sort Sasaki, Hiroyuki
collection PubMed
description Water-soluble dietary fiber is known to modulate fecal microbiota. Although there are a few reports investigating the effects of fiber intake timing on metabolism, there are none on the effect of intake timing on microbiota. Therefore, in this study, we examined the timing effects of inulin-containing food on fecal microbiota. Mice were housed under conditions with a two-meals-per-day schedule, with a long fasting period in the morning and a short fasting period in the evening. Then, 10–14 days after inulin intake, cecal content and feces were collected, and cecal pH and short-chain fatty acids (SCFAs) were measured. The microbiome was determined using 16S rDNA sequencing. Inulin feeding in the morning rather than the evening decreased the cecal pH, increased SCFAs, and changed the microbiome composition. These data suggest that inulin is more easily digested by fecal microbiota during the active period than the inactive period. Furthermore, to confirm the effect of fasting length, mice were housed under a one-meal-per-day schedule. When the duration of fasting was equal, the difference between morning and evening nearly disappeared. Thus, our study demonstrates that consuming inulin at breakfast, which is generally after a longer fasting period, has a greater effect on the microbiota.
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spelling pubmed-68937282019-12-23 Mice Microbiota Composition Changes by Inulin Feeding with a Long Fasting Period under a Two-Meals-Per-Day Schedule Sasaki, Hiroyuki Miyakawa, Hiroki Watanabe, Aya Nakayama, Yuki Lyu, Yijin Hama, Koki Shibata, Shigenobu Nutrients Article Water-soluble dietary fiber is known to modulate fecal microbiota. Although there are a few reports investigating the effects of fiber intake timing on metabolism, there are none on the effect of intake timing on microbiota. Therefore, in this study, we examined the timing effects of inulin-containing food on fecal microbiota. Mice were housed under conditions with a two-meals-per-day schedule, with a long fasting period in the morning and a short fasting period in the evening. Then, 10–14 days after inulin intake, cecal content and feces were collected, and cecal pH and short-chain fatty acids (SCFAs) were measured. The microbiome was determined using 16S rDNA sequencing. Inulin feeding in the morning rather than the evening decreased the cecal pH, increased SCFAs, and changed the microbiome composition. These data suggest that inulin is more easily digested by fecal microbiota during the active period than the inactive period. Furthermore, to confirm the effect of fasting length, mice were housed under a one-meal-per-day schedule. When the duration of fasting was equal, the difference between morning and evening nearly disappeared. Thus, our study demonstrates that consuming inulin at breakfast, which is generally after a longer fasting period, has a greater effect on the microbiota. MDPI 2019-11-16 /pmc/articles/PMC6893728/ /pubmed/31744168 http://dx.doi.org/10.3390/nu11112802 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sasaki, Hiroyuki
Miyakawa, Hiroki
Watanabe, Aya
Nakayama, Yuki
Lyu, Yijin
Hama, Koki
Shibata, Shigenobu
Mice Microbiota Composition Changes by Inulin Feeding with a Long Fasting Period under a Two-Meals-Per-Day Schedule
title Mice Microbiota Composition Changes by Inulin Feeding with a Long Fasting Period under a Two-Meals-Per-Day Schedule
title_full Mice Microbiota Composition Changes by Inulin Feeding with a Long Fasting Period under a Two-Meals-Per-Day Schedule
title_fullStr Mice Microbiota Composition Changes by Inulin Feeding with a Long Fasting Period under a Two-Meals-Per-Day Schedule
title_full_unstemmed Mice Microbiota Composition Changes by Inulin Feeding with a Long Fasting Period under a Two-Meals-Per-Day Schedule
title_short Mice Microbiota Composition Changes by Inulin Feeding with a Long Fasting Period under a Two-Meals-Per-Day Schedule
title_sort mice microbiota composition changes by inulin feeding with a long fasting period under a two-meals-per-day schedule
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893728/
https://www.ncbi.nlm.nih.gov/pubmed/31744168
http://dx.doi.org/10.3390/nu11112802
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