Cargando…

High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses

Victoria and Yamagata lineages of influenza B viruses are globally circulating in seasonal epidemics. Madin–Darby canine kidney (MDCK) cells are permissive for viral isolation and vaccine manufacture. Nevertheless, the interplay between influenza B viruses and host microRNAs has not been investigate...

Descripción completa

Detalles Bibliográficos
Autores principales: Saengchoowong, Suthat, Khongnomnan, Kritsada, Poomipak, Witthaya, Praianantathavorn, Kesmanee, Poovorawan, Yong, Zhang, Qibo, Payungporn, Sunchai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893747/
https://www.ncbi.nlm.nih.gov/pubmed/31717720
http://dx.doi.org/10.3390/v11110986
_version_ 1783476271224717312
author Saengchoowong, Suthat
Khongnomnan, Kritsada
Poomipak, Witthaya
Praianantathavorn, Kesmanee
Poovorawan, Yong
Zhang, Qibo
Payungporn, Sunchai
author_facet Saengchoowong, Suthat
Khongnomnan, Kritsada
Poomipak, Witthaya
Praianantathavorn, Kesmanee
Poovorawan, Yong
Zhang, Qibo
Payungporn, Sunchai
author_sort Saengchoowong, Suthat
collection PubMed
description Victoria and Yamagata lineages of influenza B viruses are globally circulating in seasonal epidemics. Madin–Darby canine kidney (MDCK) cells are permissive for viral isolation and vaccine manufacture. Nevertheless, the interplay between influenza B viruses and host microRNAs has not been investigated in this cell line. Therefore, the present study aims at high-throughput analysis of canine microRNA profile upon infection of influenza B viruses. Briefly, MDCK cells were infected with Victoria or Yamagata lineage at MOI of 0.01. After being harvested at 6, 12 and 24 h post infection, microRNAs were subjected to high-throughput sequencing based on MiSeq platform (Illumina). The results demonstrated that five microRNAs including cfa-miR-197, cfa-miR-215, cfa-miR361, cfa-miR-1841, and cfa-miR-1842 were overexpressed in both Victoria and Yamagata lineage infections. Interestingly, computational prediction showed that karyopherin alpha 6 (KPNA6) was targeted by cfa-miR-197 and cfa-miR-215. Moreover, the binding sites of both microRNAs were assessed by 3′-UTR reporter assay. The results showed that only cfa-miR-197 could bind to the target sites of KPNA6, leading to suppressing luciferase activity. Additionally, silencing of KPNA6 was confirmed by overexpression of cfa-miR-197. This study provides canine microRNA responses to seasonal influenza B viruses, suggesting that virus-mediated microRNAs might play crucial roles in host gene regulation.
format Online
Article
Text
id pubmed-6893747
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-68937472019-12-23 High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses Saengchoowong, Suthat Khongnomnan, Kritsada Poomipak, Witthaya Praianantathavorn, Kesmanee Poovorawan, Yong Zhang, Qibo Payungporn, Sunchai Viruses Article Victoria and Yamagata lineages of influenza B viruses are globally circulating in seasonal epidemics. Madin–Darby canine kidney (MDCK) cells are permissive for viral isolation and vaccine manufacture. Nevertheless, the interplay between influenza B viruses and host microRNAs has not been investigated in this cell line. Therefore, the present study aims at high-throughput analysis of canine microRNA profile upon infection of influenza B viruses. Briefly, MDCK cells were infected with Victoria or Yamagata lineage at MOI of 0.01. After being harvested at 6, 12 and 24 h post infection, microRNAs were subjected to high-throughput sequencing based on MiSeq platform (Illumina). The results demonstrated that five microRNAs including cfa-miR-197, cfa-miR-215, cfa-miR361, cfa-miR-1841, and cfa-miR-1842 were overexpressed in both Victoria and Yamagata lineage infections. Interestingly, computational prediction showed that karyopherin alpha 6 (KPNA6) was targeted by cfa-miR-197 and cfa-miR-215. Moreover, the binding sites of both microRNAs were assessed by 3′-UTR reporter assay. The results showed that only cfa-miR-197 could bind to the target sites of KPNA6, leading to suppressing luciferase activity. Additionally, silencing of KPNA6 was confirmed by overexpression of cfa-miR-197. This study provides canine microRNA responses to seasonal influenza B viruses, suggesting that virus-mediated microRNAs might play crucial roles in host gene regulation. MDPI 2019-10-25 /pmc/articles/PMC6893747/ /pubmed/31717720 http://dx.doi.org/10.3390/v11110986 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Saengchoowong, Suthat
Khongnomnan, Kritsada
Poomipak, Witthaya
Praianantathavorn, Kesmanee
Poovorawan, Yong
Zhang, Qibo
Payungporn, Sunchai
High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses
title High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses
title_full High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses
title_fullStr High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses
title_full_unstemmed High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses
title_short High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses
title_sort high-throughput microrna profiles of permissive madin-darby canine kidney cell line infected with influenza b viruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893747/
https://www.ncbi.nlm.nih.gov/pubmed/31717720
http://dx.doi.org/10.3390/v11110986
work_keys_str_mv AT saengchoowongsuthat highthroughputmicrornaprofilesofpermissivemadindarbycaninekidneycelllineinfectedwithinfluenzabviruses
AT khongnomnankritsada highthroughputmicrornaprofilesofpermissivemadindarbycaninekidneycelllineinfectedwithinfluenzabviruses
AT poomipakwitthaya highthroughputmicrornaprofilesofpermissivemadindarbycaninekidneycelllineinfectedwithinfluenzabviruses
AT praianantathavornkesmanee highthroughputmicrornaprofilesofpermissivemadindarbycaninekidneycelllineinfectedwithinfluenzabviruses
AT poovorawanyong highthroughputmicrornaprofilesofpermissivemadindarbycaninekidneycelllineinfectedwithinfluenzabviruses
AT zhangqibo highthroughputmicrornaprofilesofpermissivemadindarbycaninekidneycelllineinfectedwithinfluenzabviruses
AT payungpornsunchai highthroughputmicrornaprofilesofpermissivemadindarbycaninekidneycelllineinfectedwithinfluenzabviruses