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Genetic, Molecular, and Pathogenic Characterization of the H9N2 Avian Influenza Viruses Currently Circulating in South China

The prevalence and variation of the H9N2 avian influenza virus (AIV) pose a threat to public health. A total of eight viruses isolated from farmed poultry in South China during 2017–2018 were selected as representative strains for further systematic study. Phylogenetic analyses indicated that these...

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Autores principales: Sun, Hailiang, Lin, Jiate, Liu, Zhiting, Yu, Yanan, Wu, Meihua, Li, Shuo, Liu, Yang, Feng, Yaling, Wu, Yuqian, Li, Mingliang, Jiao, Peirong, Luo, Kaijian, Liao, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893773/
https://www.ncbi.nlm.nih.gov/pubmed/31717393
http://dx.doi.org/10.3390/v11111040
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author Sun, Hailiang
Lin, Jiate
Liu, Zhiting
Yu, Yanan
Wu, Meihua
Li, Shuo
Liu, Yang
Feng, Yaling
Wu, Yuqian
Li, Mingliang
Jiao, Peirong
Luo, Kaijian
Liao, Ming
author_facet Sun, Hailiang
Lin, Jiate
Liu, Zhiting
Yu, Yanan
Wu, Meihua
Li, Shuo
Liu, Yang
Feng, Yaling
Wu, Yuqian
Li, Mingliang
Jiao, Peirong
Luo, Kaijian
Liao, Ming
author_sort Sun, Hailiang
collection PubMed
description The prevalence and variation of the H9N2 avian influenza virus (AIV) pose a threat to public health. A total of eight viruses isolated from farmed poultry in South China during 2017–2018 were selected as representative strains for further systematic study. Phylogenetic analyses indicated that these prevalent viruses belong to the Y280-like lineage and that the internal genes are highly similar to those of recently circulating human H7N9 viruses. The receptor-binding assay showed that most of the H9N2 isolates preferentially bound to the human-like receptor, increasing the risk of them crossing the species barrier and causing human infection. Our in vitro, multi-step growth curve results indicate these viruses can effectively replicate in mammalian cells. Infection in mice showed that three viruses effectively replicated in the lung of mice. Infection in swine revealed that the viruses readily replicated in the upper respiratory tract of pig and effectively induced viral shedding. Our findings suggested that the H9N2 AIVs circulating in poultry recently acquired an enhanced ability to transmit from avian to mammalians, including humans. Based on our findings, we propose that it is essential to strengthen the efforts to surveil and test the pathogenicity of H9N2 AIVs.
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spelling pubmed-68937732019-12-23 Genetic, Molecular, and Pathogenic Characterization of the H9N2 Avian Influenza Viruses Currently Circulating in South China Sun, Hailiang Lin, Jiate Liu, Zhiting Yu, Yanan Wu, Meihua Li, Shuo Liu, Yang Feng, Yaling Wu, Yuqian Li, Mingliang Jiao, Peirong Luo, Kaijian Liao, Ming Viruses Article The prevalence and variation of the H9N2 avian influenza virus (AIV) pose a threat to public health. A total of eight viruses isolated from farmed poultry in South China during 2017–2018 were selected as representative strains for further systematic study. Phylogenetic analyses indicated that these prevalent viruses belong to the Y280-like lineage and that the internal genes are highly similar to those of recently circulating human H7N9 viruses. The receptor-binding assay showed that most of the H9N2 isolates preferentially bound to the human-like receptor, increasing the risk of them crossing the species barrier and causing human infection. Our in vitro, multi-step growth curve results indicate these viruses can effectively replicate in mammalian cells. Infection in mice showed that three viruses effectively replicated in the lung of mice. Infection in swine revealed that the viruses readily replicated in the upper respiratory tract of pig and effectively induced viral shedding. Our findings suggested that the H9N2 AIVs circulating in poultry recently acquired an enhanced ability to transmit from avian to mammalians, including humans. Based on our findings, we propose that it is essential to strengthen the efforts to surveil and test the pathogenicity of H9N2 AIVs. MDPI 2019-11-08 /pmc/articles/PMC6893773/ /pubmed/31717393 http://dx.doi.org/10.3390/v11111040 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sun, Hailiang
Lin, Jiate
Liu, Zhiting
Yu, Yanan
Wu, Meihua
Li, Shuo
Liu, Yang
Feng, Yaling
Wu, Yuqian
Li, Mingliang
Jiao, Peirong
Luo, Kaijian
Liao, Ming
Genetic, Molecular, and Pathogenic Characterization of the H9N2 Avian Influenza Viruses Currently Circulating in South China
title Genetic, Molecular, and Pathogenic Characterization of the H9N2 Avian Influenza Viruses Currently Circulating in South China
title_full Genetic, Molecular, and Pathogenic Characterization of the H9N2 Avian Influenza Viruses Currently Circulating in South China
title_fullStr Genetic, Molecular, and Pathogenic Characterization of the H9N2 Avian Influenza Viruses Currently Circulating in South China
title_full_unstemmed Genetic, Molecular, and Pathogenic Characterization of the H9N2 Avian Influenza Viruses Currently Circulating in South China
title_short Genetic, Molecular, and Pathogenic Characterization of the H9N2 Avian Influenza Viruses Currently Circulating in South China
title_sort genetic, molecular, and pathogenic characterization of the h9n2 avian influenza viruses currently circulating in south china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893773/
https://www.ncbi.nlm.nih.gov/pubmed/31717393
http://dx.doi.org/10.3390/v11111040
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