Cargando…
In Vivo Characterization of Tick-Borne Encephalitis Virus in Bank Voles (Myodes glareolus)
Tick-borne encephalitis is the most important tick-transmitted zoonotic virus infection in Eurasia, causing severe neurological symptoms in humans. The causative agent, the tick-borne encephalitis virus (TBEV), circulates between ticks and a variety of mammalian hosts. To study the interaction betwe...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893798/ https://www.ncbi.nlm.nih.gov/pubmed/31731773 http://dx.doi.org/10.3390/v11111069 |
_version_ | 1783476283276001280 |
---|---|
author | Michelitsch, Anna Tews, Birke Andrea Klaus, Christine Bestehorn-Willmann, Malena Dobler, Gerhard Beer, Martin Wernike, Kerstin |
author_facet | Michelitsch, Anna Tews, Birke Andrea Klaus, Christine Bestehorn-Willmann, Malena Dobler, Gerhard Beer, Martin Wernike, Kerstin |
author_sort | Michelitsch, Anna |
collection | PubMed |
description | Tick-borne encephalitis is the most important tick-transmitted zoonotic virus infection in Eurasia, causing severe neurological symptoms in humans. The causative agent, the tick-borne encephalitis virus (TBEV), circulates between ticks and a variety of mammalian hosts. To study the interaction between TBEV and one of its suspected reservoir hosts, bank voles of the Western evolutionary lineage were inoculated subcutaneously with either one of eight TBEV strains or the related attenuated Langat virus, and were euthanized after 28 days. In addition, a subset of four strains was characterized in bank voles of the Carpathian linage. Six bank voles were inoculated per strain, and were housed together in groups of three with one uninfected in-contact animal each. Generally, most bank voles did not show any clinical signs over the course of infection. However, one infected bank vole died and three had to be euthanized prematurely, all of which had been inoculated with the identical TBEV strain (Battaune 17-H9, isolated in 2017 in Germany from a bank vole). All inoculated animals seroconverted, while none of the in-contact animals did. Viral RNA was detected via real-time RT-PCR in the whole blood samples of 31 out of 74 inoculated and surviving bank voles. The corresponding serum sample remained PCR-negative in nearly all cases (29/31). In addition, brain and/or spine samples tested positive in 11 cases, mostly correlating with a positive whole blood sample. Our findings suggest a good adaption of TBEV to bank voles, combining in most cases a low virulence phenotype with detectable virus replication and hinting at a reservoir host function of bank voles for TBEV. |
format | Online Article Text |
id | pubmed-6893798 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68937982019-12-23 In Vivo Characterization of Tick-Borne Encephalitis Virus in Bank Voles (Myodes glareolus) Michelitsch, Anna Tews, Birke Andrea Klaus, Christine Bestehorn-Willmann, Malena Dobler, Gerhard Beer, Martin Wernike, Kerstin Viruses Article Tick-borne encephalitis is the most important tick-transmitted zoonotic virus infection in Eurasia, causing severe neurological symptoms in humans. The causative agent, the tick-borne encephalitis virus (TBEV), circulates between ticks and a variety of mammalian hosts. To study the interaction between TBEV and one of its suspected reservoir hosts, bank voles of the Western evolutionary lineage were inoculated subcutaneously with either one of eight TBEV strains or the related attenuated Langat virus, and were euthanized after 28 days. In addition, a subset of four strains was characterized in bank voles of the Carpathian linage. Six bank voles were inoculated per strain, and were housed together in groups of three with one uninfected in-contact animal each. Generally, most bank voles did not show any clinical signs over the course of infection. However, one infected bank vole died and three had to be euthanized prematurely, all of which had been inoculated with the identical TBEV strain (Battaune 17-H9, isolated in 2017 in Germany from a bank vole). All inoculated animals seroconverted, while none of the in-contact animals did. Viral RNA was detected via real-time RT-PCR in the whole blood samples of 31 out of 74 inoculated and surviving bank voles. The corresponding serum sample remained PCR-negative in nearly all cases (29/31). In addition, brain and/or spine samples tested positive in 11 cases, mostly correlating with a positive whole blood sample. Our findings suggest a good adaption of TBEV to bank voles, combining in most cases a low virulence phenotype with detectable virus replication and hinting at a reservoir host function of bank voles for TBEV. MDPI 2019-11-15 /pmc/articles/PMC6893798/ /pubmed/31731773 http://dx.doi.org/10.3390/v11111069 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Michelitsch, Anna Tews, Birke Andrea Klaus, Christine Bestehorn-Willmann, Malena Dobler, Gerhard Beer, Martin Wernike, Kerstin In Vivo Characterization of Tick-Borne Encephalitis Virus in Bank Voles (Myodes glareolus) |
title | In Vivo Characterization of Tick-Borne Encephalitis Virus in Bank Voles (Myodes glareolus) |
title_full | In Vivo Characterization of Tick-Borne Encephalitis Virus in Bank Voles (Myodes glareolus) |
title_fullStr | In Vivo Characterization of Tick-Borne Encephalitis Virus in Bank Voles (Myodes glareolus) |
title_full_unstemmed | In Vivo Characterization of Tick-Borne Encephalitis Virus in Bank Voles (Myodes glareolus) |
title_short | In Vivo Characterization of Tick-Borne Encephalitis Virus in Bank Voles (Myodes glareolus) |
title_sort | in vivo characterization of tick-borne encephalitis virus in bank voles (myodes glareolus) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6893798/ https://www.ncbi.nlm.nih.gov/pubmed/31731773 http://dx.doi.org/10.3390/v11111069 |
work_keys_str_mv | AT michelitschanna invivocharacterizationoftickborneencephalitisvirusinbankvolesmyodesglareolus AT tewsbirkeandrea invivocharacterizationoftickborneencephalitisvirusinbankvolesmyodesglareolus AT klauschristine invivocharacterizationoftickborneencephalitisvirusinbankvolesmyodesglareolus AT bestehornwillmannmalena invivocharacterizationoftickborneencephalitisvirusinbankvolesmyodesglareolus AT doblergerhard invivocharacterizationoftickborneencephalitisvirusinbankvolesmyodesglareolus AT beermartin invivocharacterizationoftickborneencephalitisvirusinbankvolesmyodesglareolus AT wernikekerstin invivocharacterizationoftickborneencephalitisvirusinbankvolesmyodesglareolus |