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Characterization of Pasteurella multocida isolated from dead rabbits with respiratory disease in Fujian, China

BACKGROUND: Pasteurella multocida is one of the important pathogens that infect rabbits, causing major economic losses in commercial rabbit farming. In this study, 205 P. multocida isolates recovered from lungs of dead rabbits with respiratory disease were defined by capsular serogroups, lipopolysac...

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Autores principales: Wang, Jinxiang, Sang, Lei, Sun, Shikun, Chen, Yanfeng, Chen, Dongjin, Xie, Xiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6894249/
https://www.ncbi.nlm.nih.gov/pubmed/31801544
http://dx.doi.org/10.1186/s12917-019-2191-3
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author Wang, Jinxiang
Sang, Lei
Sun, Shikun
Chen, Yanfeng
Chen, Dongjin
Xie, Xiping
author_facet Wang, Jinxiang
Sang, Lei
Sun, Shikun
Chen, Yanfeng
Chen, Dongjin
Xie, Xiping
author_sort Wang, Jinxiang
collection PubMed
description BACKGROUND: Pasteurella multocida is one of the important pathogens that infect rabbits, causing major economic losses in commercial rabbit farming. In this study, 205 P. multocida isolates recovered from lungs of dead rabbits with respiratory disease were defined by capsular serogroups, lipopolysaccharide (LPS) genotypes, multi-locus sequence types and screened virulence factors by using PCR assays, and tested antimicrobial susceptibility. RESULTS: The 205 isolates were assigned into 2 capsular types, A and D, and 2 LPS genotypes, L3 and L6. When combining capsular types with LPS genotypes, 4 serotypes were detected. A:L3 (51.22%, 105/205) was the most predominant serotype, followed by A:L6 (24.88%, 51/205), D:L6 (19.02%, 39/205) and D:L3 (4.88%, 10/205). The 205 isolates were grouped into 3 sequence types, ST10, ST11 and ST12. ST12 (56.10%, 115/205) was the most prevalent sequence type, followed by ST10 (24.88%, 51/205) and ST11 (19.02%, 39/205). In the 205 isolates, virulence associated genes ptfA, fur, hgbB, ompA, ompH and oma87 were positive in the PCR screening, whereas the toxA and tbpA genes were negative. Notably, the 156 capsular serogroup A isolates carried the pmHAS gene. All the 205 isolates were susceptible to most of the used antibiotics, except for streptomycin, gentamycin, kanamycin and ceftriaxone, and the resistance rates of which were 27.80, 15.61, 9.27 and 2.44%, respectively. CONCLUSIONS: This study, for the first time, described the prevalence and characteristics of P. multocida causing respiratory disease in rabbits in Fujian Province, which might be useful for tracking the epidemic strains and development of efficient vaccines and methods to prevent and control the pathogen.
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spelling pubmed-68942492019-12-11 Characterization of Pasteurella multocida isolated from dead rabbits with respiratory disease in Fujian, China Wang, Jinxiang Sang, Lei Sun, Shikun Chen, Yanfeng Chen, Dongjin Xie, Xiping BMC Vet Res Research Article BACKGROUND: Pasteurella multocida is one of the important pathogens that infect rabbits, causing major economic losses in commercial rabbit farming. In this study, 205 P. multocida isolates recovered from lungs of dead rabbits with respiratory disease were defined by capsular serogroups, lipopolysaccharide (LPS) genotypes, multi-locus sequence types and screened virulence factors by using PCR assays, and tested antimicrobial susceptibility. RESULTS: The 205 isolates were assigned into 2 capsular types, A and D, and 2 LPS genotypes, L3 and L6. When combining capsular types with LPS genotypes, 4 serotypes were detected. A:L3 (51.22%, 105/205) was the most predominant serotype, followed by A:L6 (24.88%, 51/205), D:L6 (19.02%, 39/205) and D:L3 (4.88%, 10/205). The 205 isolates were grouped into 3 sequence types, ST10, ST11 and ST12. ST12 (56.10%, 115/205) was the most prevalent sequence type, followed by ST10 (24.88%, 51/205) and ST11 (19.02%, 39/205). In the 205 isolates, virulence associated genes ptfA, fur, hgbB, ompA, ompH and oma87 were positive in the PCR screening, whereas the toxA and tbpA genes were negative. Notably, the 156 capsular serogroup A isolates carried the pmHAS gene. All the 205 isolates were susceptible to most of the used antibiotics, except for streptomycin, gentamycin, kanamycin and ceftriaxone, and the resistance rates of which were 27.80, 15.61, 9.27 and 2.44%, respectively. CONCLUSIONS: This study, for the first time, described the prevalence and characteristics of P. multocida causing respiratory disease in rabbits in Fujian Province, which might be useful for tracking the epidemic strains and development of efficient vaccines and methods to prevent and control the pathogen. BioMed Central 2019-12-04 /pmc/articles/PMC6894249/ /pubmed/31801544 http://dx.doi.org/10.1186/s12917-019-2191-3 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Wang, Jinxiang
Sang, Lei
Sun, Shikun
Chen, Yanfeng
Chen, Dongjin
Xie, Xiping
Characterization of Pasteurella multocida isolated from dead rabbits with respiratory disease in Fujian, China
title Characterization of Pasteurella multocida isolated from dead rabbits with respiratory disease in Fujian, China
title_full Characterization of Pasteurella multocida isolated from dead rabbits with respiratory disease in Fujian, China
title_fullStr Characterization of Pasteurella multocida isolated from dead rabbits with respiratory disease in Fujian, China
title_full_unstemmed Characterization of Pasteurella multocida isolated from dead rabbits with respiratory disease in Fujian, China
title_short Characterization of Pasteurella multocida isolated from dead rabbits with respiratory disease in Fujian, China
title_sort characterization of pasteurella multocida isolated from dead rabbits with respiratory disease in fujian, china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6894249/
https://www.ncbi.nlm.nih.gov/pubmed/31801544
http://dx.doi.org/10.1186/s12917-019-2191-3
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