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Effects of marker type and filtering criteria on Q(ST)-F(ST) comparisons
Comparative studies of quantitative and neutral genetic differentiation (Q(ST)-F(ST) tests) provide means to detect adaptive population differentiation. However, Q(ST)-F(ST) tests can be overly liberal if the markers used deflate F(ST) below its expectation, or overly conservative if methodological...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6894560/ https://www.ncbi.nlm.nih.gov/pubmed/31827824 http://dx.doi.org/10.1098/rsos.190666 |
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author | Li, Zitong Löytynoja, Ari Fraimout, Antoine Merilä, Juha |
author_facet | Li, Zitong Löytynoja, Ari Fraimout, Antoine Merilä, Juha |
author_sort | Li, Zitong |
collection | PubMed |
description | Comparative studies of quantitative and neutral genetic differentiation (Q(ST)-F(ST) tests) provide means to detect adaptive population differentiation. However, Q(ST)-F(ST) tests can be overly liberal if the markers used deflate F(ST) below its expectation, or overly conservative if methodological biases lead to inflated F(ST) estimates. We investigated how marker type and filtering criteria for marker selection influence Q(ST)-F(ST) comparisons through their effects on F(ST) using simulations and empirical data on over 18 000 in silico genotyped microsatellites and 3.8 million single-locus polymorphism (SNP) loci from four populations of nine-spined sticklebacks (Pungitius pungitius). Empirical and simulated data revealed that F(ST) decreased with increasing marker variability, and was generally higher with SNPs than with microsatellites. The estimated baseline F(ST) levels were also sensitive to filtering criteria for SNPs: both minor alleles and linkage disequilibrium (LD) pruning influenced F(ST) estimation, as did marker ascertainment. However, in the case of stickleback data used here where Q(ST) is high, the choice of marker type, their genomic location, ascertainment and filtering made little difference to outcomes of Q(ST)-F(ST) tests. Nevertheless, we recommend that Q(ST)-F(ST) tests using microsatellites should discard the most variable loci, and those using SNPs should pay attention to marker ascertainment and properly account for LD before filtering SNPs. This may be especially important when level of quantitative trait differentiation is low and levels of neutral differentiation high. |
format | Online Article Text |
id | pubmed-6894560 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-68945602019-12-11 Effects of marker type and filtering criteria on Q(ST)-F(ST) comparisons Li, Zitong Löytynoja, Ari Fraimout, Antoine Merilä, Juha R Soc Open Sci Genetics and Genomics Comparative studies of quantitative and neutral genetic differentiation (Q(ST)-F(ST) tests) provide means to detect adaptive population differentiation. However, Q(ST)-F(ST) tests can be overly liberal if the markers used deflate F(ST) below its expectation, or overly conservative if methodological biases lead to inflated F(ST) estimates. We investigated how marker type and filtering criteria for marker selection influence Q(ST)-F(ST) comparisons through their effects on F(ST) using simulations and empirical data on over 18 000 in silico genotyped microsatellites and 3.8 million single-locus polymorphism (SNP) loci from four populations of nine-spined sticklebacks (Pungitius pungitius). Empirical and simulated data revealed that F(ST) decreased with increasing marker variability, and was generally higher with SNPs than with microsatellites. The estimated baseline F(ST) levels were also sensitive to filtering criteria for SNPs: both minor alleles and linkage disequilibrium (LD) pruning influenced F(ST) estimation, as did marker ascertainment. However, in the case of stickleback data used here where Q(ST) is high, the choice of marker type, their genomic location, ascertainment and filtering made little difference to outcomes of Q(ST)-F(ST) tests. Nevertheless, we recommend that Q(ST)-F(ST) tests using microsatellites should discard the most variable loci, and those using SNPs should pay attention to marker ascertainment and properly account for LD before filtering SNPs. This may be especially important when level of quantitative trait differentiation is low and levels of neutral differentiation high. The Royal Society 2019-11-06 /pmc/articles/PMC6894560/ /pubmed/31827824 http://dx.doi.org/10.1098/rsos.190666 Text en © 2019 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Genetics and Genomics Li, Zitong Löytynoja, Ari Fraimout, Antoine Merilä, Juha Effects of marker type and filtering criteria on Q(ST)-F(ST) comparisons |
title | Effects of marker type and filtering criteria on Q(ST)-F(ST) comparisons |
title_full | Effects of marker type and filtering criteria on Q(ST)-F(ST) comparisons |
title_fullStr | Effects of marker type and filtering criteria on Q(ST)-F(ST) comparisons |
title_full_unstemmed | Effects of marker type and filtering criteria on Q(ST)-F(ST) comparisons |
title_short | Effects of marker type and filtering criteria on Q(ST)-F(ST) comparisons |
title_sort | effects of marker type and filtering criteria on q(st)-f(st) comparisons |
topic | Genetics and Genomics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6894560/ https://www.ncbi.nlm.nih.gov/pubmed/31827824 http://dx.doi.org/10.1098/rsos.190666 |
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