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Anti-brain cancer activity of chloroform and hexane extracts of Tinospora cordifolia Miers: an in vitro perspective
BACKGROUND: Plants have been suggested as safest source of therapeutic agents, with multi targeted mode of action and least side effects. Tinospora cordifolia, commonly known as Guduchi in India, is one of the most highly valued herbs in Ayurvedic medicine. It possesses potential anti-cancer, anti-i...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Indian Academy of Neurosciences
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6894632/ https://www.ncbi.nlm.nih.gov/pubmed/31975767 http://dx.doi.org/10.5214/ans.0972.7531.260104 |
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author | Sharma, Anuradha Saggu, Sandeep Kaur Mishra, Rachana Kaur, Gurcharan |
author_facet | Sharma, Anuradha Saggu, Sandeep Kaur Mishra, Rachana Kaur, Gurcharan |
author_sort | Sharma, Anuradha |
collection | PubMed |
description | BACKGROUND: Plants have been suggested as safest source of therapeutic agents, with multi targeted mode of action and least side effects. Tinospora cordifolia, commonly known as Guduchi in India, is one of the most highly valued herbs in Ayurvedic medicine. It possesses potential anti-cancer, anti-inflammatory, hepatoprotective, anti-diabetic, immune-stimulatory and various other beneficial activities. PURPOSE: The present study was aimed to investigate the differentiation inducing potential of chloroform and hexane extracts of T. cordifolia using U87MG glioblastoma and IMR-32 neuroblastoma cell lines as model system. RESULTS: Chloroform (Chl-TCE) and hexane (Hex-TCE) extracts significantly reduced the rate of proliferation and induced cell differentiation as evidenced by MTT assay and immunostaining for GFAP and MAP-2 in glioblastoma and neuroblastoma, respectively. Further these extracts increased the expression of stress markers HSP70 and Mortalin and induced senescence. Chloroform and hexane extracts also inhibited the migration of U87MG glioblastoma and IMR-32 neuroblastoma as indicated by wound scratch assay and supported by reduced expression of NCAM. Furthermore these extracts are not toxic to normal cells as they showed no inhibitory effects on primary astrocytic and neuronal cultures. CONCLUSIONS: The present study suggests that chloroform and hexane extracts of T. cordifolia retard the rate of proliferation, induce differentiation and inhibit migration of human glioblastomas and neuroblastomas, thus may act as potential phytotherapeutic intervention in treatment of neural cancers. |
format | Online Article Text |
id | pubmed-6894632 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Indian Academy of Neurosciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-68946322020-01-23 Anti-brain cancer activity of chloroform and hexane extracts of Tinospora cordifolia Miers: an in vitro perspective Sharma, Anuradha Saggu, Sandeep Kaur Mishra, Rachana Kaur, Gurcharan Ann Neurosci Research Article BACKGROUND: Plants have been suggested as safest source of therapeutic agents, with multi targeted mode of action and least side effects. Tinospora cordifolia, commonly known as Guduchi in India, is one of the most highly valued herbs in Ayurvedic medicine. It possesses potential anti-cancer, anti-inflammatory, hepatoprotective, anti-diabetic, immune-stimulatory and various other beneficial activities. PURPOSE: The present study was aimed to investigate the differentiation inducing potential of chloroform and hexane extracts of T. cordifolia using U87MG glioblastoma and IMR-32 neuroblastoma cell lines as model system. RESULTS: Chloroform (Chl-TCE) and hexane (Hex-TCE) extracts significantly reduced the rate of proliferation and induced cell differentiation as evidenced by MTT assay and immunostaining for GFAP and MAP-2 in glioblastoma and neuroblastoma, respectively. Further these extracts increased the expression of stress markers HSP70 and Mortalin and induced senescence. Chloroform and hexane extracts also inhibited the migration of U87MG glioblastoma and IMR-32 neuroblastoma as indicated by wound scratch assay and supported by reduced expression of NCAM. Furthermore these extracts are not toxic to normal cells as they showed no inhibitory effects on primary astrocytic and neuronal cultures. CONCLUSIONS: The present study suggests that chloroform and hexane extracts of T. cordifolia retard the rate of proliferation, induce differentiation and inhibit migration of human glioblastomas and neuroblastomas, thus may act as potential phytotherapeutic intervention in treatment of neural cancers. Indian Academy of Neurosciences 2019-01-01 2019-01 /pmc/articles/PMC6894632/ /pubmed/31975767 http://dx.doi.org/10.5214/ans.0972.7531.260104 Text en Copyright © 2019, Annals of Neurosciences http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Sharma, Anuradha Saggu, Sandeep Kaur Mishra, Rachana Kaur, Gurcharan Anti-brain cancer activity of chloroform and hexane extracts of Tinospora cordifolia Miers: an in vitro perspective |
title | Anti-brain cancer activity of chloroform and hexane extracts of
Tinospora cordifolia Miers: an in vitro
perspective |
title_full | Anti-brain cancer activity of chloroform and hexane extracts of
Tinospora cordifolia Miers: an in vitro
perspective |
title_fullStr | Anti-brain cancer activity of chloroform and hexane extracts of
Tinospora cordifolia Miers: an in vitro
perspective |
title_full_unstemmed | Anti-brain cancer activity of chloroform and hexane extracts of
Tinospora cordifolia Miers: an in vitro
perspective |
title_short | Anti-brain cancer activity of chloroform and hexane extracts of
Tinospora cordifolia Miers: an in vitro
perspective |
title_sort | anti-brain cancer activity of chloroform and hexane extracts of
tinospora cordifolia miers: an in vitro
perspective |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6894632/ https://www.ncbi.nlm.nih.gov/pubmed/31975767 http://dx.doi.org/10.5214/ans.0972.7531.260104 |
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