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An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function

Chloroplasts are important for photosynthesis and for plant immunity against microbial pathogens. Here we identify a haustorium-specific protein (Pst_12806) from the wheat stripe rust fungus, Puccinia striiformis f. sp. tritici (Pst), that is translocated into chloroplasts and affects chloroplast fu...

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Autores principales: Xu, Qiang, Tang, Chunlei, Wang, Xiaodong, Sun, Shutian, Zhao, Jinren, Kang, Zhensheng, Wang, Xiaojie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895047/
https://www.ncbi.nlm.nih.gov/pubmed/31804478
http://dx.doi.org/10.1038/s41467-019-13487-6
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author Xu, Qiang
Tang, Chunlei
Wang, Xiaodong
Sun, Shutian
Zhao, Jinren
Kang, Zhensheng
Wang, Xiaojie
author_facet Xu, Qiang
Tang, Chunlei
Wang, Xiaodong
Sun, Shutian
Zhao, Jinren
Kang, Zhensheng
Wang, Xiaojie
author_sort Xu, Qiang
collection PubMed
description Chloroplasts are important for photosynthesis and for plant immunity against microbial pathogens. Here we identify a haustorium-specific protein (Pst_12806) from the wheat stripe rust fungus, Puccinia striiformis f. sp. tritici (Pst), that is translocated into chloroplasts and affects chloroplast function. Transient expression of Pst_12806 inhibits BAX-induced cell death in tobacco plants and reduces Pseudomonas-induced hypersensitive response in wheat. It suppresses plant basal immunity by reducing callose deposition and the expression of defense-related genes. Pst_12806 is upregulated during infection, and its knockdown (by host-induced gene silencing) reduces Pst growth and development, likely due to increased ROS accumulation. Pst_12806 interacts with the C-terminal Rieske domain of the wheat TaISP protein (a putative component of the cytochrome b6-f complex). Expression of Pst_12806 in plants reduces electron transport rate, photosynthesis, and production of chloroplast-derived ROS. Silencing TaISP by virus-induced gene silencing in a susceptible wheat cultivar reduces fungal growth and uredinium development, suggesting an increase in resistance against Pst infection.
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spelling pubmed-68950472019-12-09 An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function Xu, Qiang Tang, Chunlei Wang, Xiaodong Sun, Shutian Zhao, Jinren Kang, Zhensheng Wang, Xiaojie Nat Commun Article Chloroplasts are important for photosynthesis and for plant immunity against microbial pathogens. Here we identify a haustorium-specific protein (Pst_12806) from the wheat stripe rust fungus, Puccinia striiformis f. sp. tritici (Pst), that is translocated into chloroplasts and affects chloroplast function. Transient expression of Pst_12806 inhibits BAX-induced cell death in tobacco plants and reduces Pseudomonas-induced hypersensitive response in wheat. It suppresses plant basal immunity by reducing callose deposition and the expression of defense-related genes. Pst_12806 is upregulated during infection, and its knockdown (by host-induced gene silencing) reduces Pst growth and development, likely due to increased ROS accumulation. Pst_12806 interacts with the C-terminal Rieske domain of the wheat TaISP protein (a putative component of the cytochrome b6-f complex). Expression of Pst_12806 in plants reduces electron transport rate, photosynthesis, and production of chloroplast-derived ROS. Silencing TaISP by virus-induced gene silencing in a susceptible wheat cultivar reduces fungal growth and uredinium development, suggesting an increase in resistance against Pst infection. Nature Publishing Group UK 2019-12-05 /pmc/articles/PMC6895047/ /pubmed/31804478 http://dx.doi.org/10.1038/s41467-019-13487-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Xu, Qiang
Tang, Chunlei
Wang, Xiaodong
Sun, Shutian
Zhao, Jinren
Kang, Zhensheng
Wang, Xiaojie
An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function
title An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function
title_full An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function
title_fullStr An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function
title_full_unstemmed An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function
title_short An effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function
title_sort effector protein of the wheat stripe rust fungus targets chloroplasts and suppresses chloroplast function
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895047/
https://www.ncbi.nlm.nih.gov/pubmed/31804478
http://dx.doi.org/10.1038/s41467-019-13487-6
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