Cargando…

Real-time reconstruction and visualisation towards dynamic feedback control during time-resolved tomography experiments at TOMCAT

Tomographic X-ray microscopy beamlines at synchrotron light sources worldwide have pushed the achievable time-resolution for dynamic 3-dimensional structural investigations down to a fraction of a second, allowing the study of quickly evolving systems. The large data rates involved impose heavy dema...

Descripción completa

Detalles Bibliográficos
Autores principales: Buurlage, Jan-Willem, Marone, Federica, Pelt, Daniël M., Palenstijn, Willem Jan, Stampanoni, Marco, Batenburg, K. Joost, Schlepütz, Christian M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895167/
https://www.ncbi.nlm.nih.gov/pubmed/31804524
http://dx.doi.org/10.1038/s41598-019-54647-4
_version_ 1783476537884934144
author Buurlage, Jan-Willem
Marone, Federica
Pelt, Daniël M.
Palenstijn, Willem Jan
Stampanoni, Marco
Batenburg, K. Joost
Schlepütz, Christian M.
author_facet Buurlage, Jan-Willem
Marone, Federica
Pelt, Daniël M.
Palenstijn, Willem Jan
Stampanoni, Marco
Batenburg, K. Joost
Schlepütz, Christian M.
author_sort Buurlage, Jan-Willem
collection PubMed
description Tomographic X-ray microscopy beamlines at synchrotron light sources worldwide have pushed the achievable time-resolution for dynamic 3-dimensional structural investigations down to a fraction of a second, allowing the study of quickly evolving systems. The large data rates involved impose heavy demands on computational resources, making it difficult to readily process and interrogate the resulting volumes. The data acquisition is thus performed essentially blindly. Such a sequential process makes it hard to notice problems with the measurement protocol or sample conditions, potentially rendering the acquired data unusable, and it keeps the user from optimizing the experimental parameters of the imaging task at hand. We present an efficient approach to address this issue based on the real-time reconstruction, visualisation and on-the-fly analysis of a small number of arbitrarily oriented slices. This solution, requiring only a single additional computing workstation, has been implemented at the TOMCAT beamline of the Swiss Light Source. The system is able to process multiple sets of slices per second, thus pushing the reconstruction throughput on the same level as the data acquisition. This enables the monitoring of dynamic processes as they occur and represents the next crucial step towards adaptive feedback control of time-resolved in situ tomographic experiments.
format Online
Article
Text
id pubmed-6895167
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-68951672019-12-12 Real-time reconstruction and visualisation towards dynamic feedback control during time-resolved tomography experiments at TOMCAT Buurlage, Jan-Willem Marone, Federica Pelt, Daniël M. Palenstijn, Willem Jan Stampanoni, Marco Batenburg, K. Joost Schlepütz, Christian M. Sci Rep Article Tomographic X-ray microscopy beamlines at synchrotron light sources worldwide have pushed the achievable time-resolution for dynamic 3-dimensional structural investigations down to a fraction of a second, allowing the study of quickly evolving systems. The large data rates involved impose heavy demands on computational resources, making it difficult to readily process and interrogate the resulting volumes. The data acquisition is thus performed essentially blindly. Such a sequential process makes it hard to notice problems with the measurement protocol or sample conditions, potentially rendering the acquired data unusable, and it keeps the user from optimizing the experimental parameters of the imaging task at hand. We present an efficient approach to address this issue based on the real-time reconstruction, visualisation and on-the-fly analysis of a small number of arbitrarily oriented slices. This solution, requiring only a single additional computing workstation, has been implemented at the TOMCAT beamline of the Swiss Light Source. The system is able to process multiple sets of slices per second, thus pushing the reconstruction throughput on the same level as the data acquisition. This enables the monitoring of dynamic processes as they occur and represents the next crucial step towards adaptive feedback control of time-resolved in situ tomographic experiments. Nature Publishing Group UK 2019-12-05 /pmc/articles/PMC6895167/ /pubmed/31804524 http://dx.doi.org/10.1038/s41598-019-54647-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Buurlage, Jan-Willem
Marone, Federica
Pelt, Daniël M.
Palenstijn, Willem Jan
Stampanoni, Marco
Batenburg, K. Joost
Schlepütz, Christian M.
Real-time reconstruction and visualisation towards dynamic feedback control during time-resolved tomography experiments at TOMCAT
title Real-time reconstruction and visualisation towards dynamic feedback control during time-resolved tomography experiments at TOMCAT
title_full Real-time reconstruction and visualisation towards dynamic feedback control during time-resolved tomography experiments at TOMCAT
title_fullStr Real-time reconstruction and visualisation towards dynamic feedback control during time-resolved tomography experiments at TOMCAT
title_full_unstemmed Real-time reconstruction and visualisation towards dynamic feedback control during time-resolved tomography experiments at TOMCAT
title_short Real-time reconstruction and visualisation towards dynamic feedback control during time-resolved tomography experiments at TOMCAT
title_sort real-time reconstruction and visualisation towards dynamic feedback control during time-resolved tomography experiments at tomcat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895167/
https://www.ncbi.nlm.nih.gov/pubmed/31804524
http://dx.doi.org/10.1038/s41598-019-54647-4
work_keys_str_mv AT buurlagejanwillem realtimereconstructionandvisualisationtowardsdynamicfeedbackcontrolduringtimeresolvedtomographyexperimentsattomcat
AT maronefederica realtimereconstructionandvisualisationtowardsdynamicfeedbackcontrolduringtimeresolvedtomographyexperimentsattomcat
AT peltdanielm realtimereconstructionandvisualisationtowardsdynamicfeedbackcontrolduringtimeresolvedtomographyexperimentsattomcat
AT palenstijnwillemjan realtimereconstructionandvisualisationtowardsdynamicfeedbackcontrolduringtimeresolvedtomographyexperimentsattomcat
AT stampanonimarco realtimereconstructionandvisualisationtowardsdynamicfeedbackcontrolduringtimeresolvedtomographyexperimentsattomcat
AT batenburgkjoost realtimereconstructionandvisualisationtowardsdynamicfeedbackcontrolduringtimeresolvedtomographyexperimentsattomcat
AT schleputzchristianm realtimereconstructionandvisualisationtowardsdynamicfeedbackcontrolduringtimeresolvedtomographyexperimentsattomcat