Cargando…
Real-time reconstruction and visualisation towards dynamic feedback control during time-resolved tomography experiments at TOMCAT
Tomographic X-ray microscopy beamlines at synchrotron light sources worldwide have pushed the achievable time-resolution for dynamic 3-dimensional structural investigations down to a fraction of a second, allowing the study of quickly evolving systems. The large data rates involved impose heavy dema...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895167/ https://www.ncbi.nlm.nih.gov/pubmed/31804524 http://dx.doi.org/10.1038/s41598-019-54647-4 |
_version_ | 1783476537884934144 |
---|---|
author | Buurlage, Jan-Willem Marone, Federica Pelt, Daniël M. Palenstijn, Willem Jan Stampanoni, Marco Batenburg, K. Joost Schlepütz, Christian M. |
author_facet | Buurlage, Jan-Willem Marone, Federica Pelt, Daniël M. Palenstijn, Willem Jan Stampanoni, Marco Batenburg, K. Joost Schlepütz, Christian M. |
author_sort | Buurlage, Jan-Willem |
collection | PubMed |
description | Tomographic X-ray microscopy beamlines at synchrotron light sources worldwide have pushed the achievable time-resolution for dynamic 3-dimensional structural investigations down to a fraction of a second, allowing the study of quickly evolving systems. The large data rates involved impose heavy demands on computational resources, making it difficult to readily process and interrogate the resulting volumes. The data acquisition is thus performed essentially blindly. Such a sequential process makes it hard to notice problems with the measurement protocol or sample conditions, potentially rendering the acquired data unusable, and it keeps the user from optimizing the experimental parameters of the imaging task at hand. We present an efficient approach to address this issue based on the real-time reconstruction, visualisation and on-the-fly analysis of a small number of arbitrarily oriented slices. This solution, requiring only a single additional computing workstation, has been implemented at the TOMCAT beamline of the Swiss Light Source. The system is able to process multiple sets of slices per second, thus pushing the reconstruction throughput on the same level as the data acquisition. This enables the monitoring of dynamic processes as they occur and represents the next crucial step towards adaptive feedback control of time-resolved in situ tomographic experiments. |
format | Online Article Text |
id | pubmed-6895167 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-68951672019-12-12 Real-time reconstruction and visualisation towards dynamic feedback control during time-resolved tomography experiments at TOMCAT Buurlage, Jan-Willem Marone, Federica Pelt, Daniël M. Palenstijn, Willem Jan Stampanoni, Marco Batenburg, K. Joost Schlepütz, Christian M. Sci Rep Article Tomographic X-ray microscopy beamlines at synchrotron light sources worldwide have pushed the achievable time-resolution for dynamic 3-dimensional structural investigations down to a fraction of a second, allowing the study of quickly evolving systems. The large data rates involved impose heavy demands on computational resources, making it difficult to readily process and interrogate the resulting volumes. The data acquisition is thus performed essentially blindly. Such a sequential process makes it hard to notice problems with the measurement protocol or sample conditions, potentially rendering the acquired data unusable, and it keeps the user from optimizing the experimental parameters of the imaging task at hand. We present an efficient approach to address this issue based on the real-time reconstruction, visualisation and on-the-fly analysis of a small number of arbitrarily oriented slices. This solution, requiring only a single additional computing workstation, has been implemented at the TOMCAT beamline of the Swiss Light Source. The system is able to process multiple sets of slices per second, thus pushing the reconstruction throughput on the same level as the data acquisition. This enables the monitoring of dynamic processes as they occur and represents the next crucial step towards adaptive feedback control of time-resolved in situ tomographic experiments. Nature Publishing Group UK 2019-12-05 /pmc/articles/PMC6895167/ /pubmed/31804524 http://dx.doi.org/10.1038/s41598-019-54647-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Buurlage, Jan-Willem Marone, Federica Pelt, Daniël M. Palenstijn, Willem Jan Stampanoni, Marco Batenburg, K. Joost Schlepütz, Christian M. Real-time reconstruction and visualisation towards dynamic feedback control during time-resolved tomography experiments at TOMCAT |
title | Real-time reconstruction and visualisation towards dynamic feedback control during time-resolved tomography experiments at TOMCAT |
title_full | Real-time reconstruction and visualisation towards dynamic feedback control during time-resolved tomography experiments at TOMCAT |
title_fullStr | Real-time reconstruction and visualisation towards dynamic feedback control during time-resolved tomography experiments at TOMCAT |
title_full_unstemmed | Real-time reconstruction and visualisation towards dynamic feedback control during time-resolved tomography experiments at TOMCAT |
title_short | Real-time reconstruction and visualisation towards dynamic feedback control during time-resolved tomography experiments at TOMCAT |
title_sort | real-time reconstruction and visualisation towards dynamic feedback control during time-resolved tomography experiments at tomcat |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895167/ https://www.ncbi.nlm.nih.gov/pubmed/31804524 http://dx.doi.org/10.1038/s41598-019-54647-4 |
work_keys_str_mv | AT buurlagejanwillem realtimereconstructionandvisualisationtowardsdynamicfeedbackcontrolduringtimeresolvedtomographyexperimentsattomcat AT maronefederica realtimereconstructionandvisualisationtowardsdynamicfeedbackcontrolduringtimeresolvedtomographyexperimentsattomcat AT peltdanielm realtimereconstructionandvisualisationtowardsdynamicfeedbackcontrolduringtimeresolvedtomographyexperimentsattomcat AT palenstijnwillemjan realtimereconstructionandvisualisationtowardsdynamicfeedbackcontrolduringtimeresolvedtomographyexperimentsattomcat AT stampanonimarco realtimereconstructionandvisualisationtowardsdynamicfeedbackcontrolduringtimeresolvedtomographyexperimentsattomcat AT batenburgkjoost realtimereconstructionandvisualisationtowardsdynamicfeedbackcontrolduringtimeresolvedtomographyexperimentsattomcat AT schleputzchristianm realtimereconstructionandvisualisationtowardsdynamicfeedbackcontrolduringtimeresolvedtomographyexperimentsattomcat |