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Fusogenic Liposomes Increase the Antimicrobial Activity of Vancomycin Against Staphylococcus aureus Biofilm

Objective: The aim of the present study was to encapsulate vancomycin in different liposomal formulations and compare the in vitro antimicrobial activity against Staphylococcus aureus biofilms. Methods: Large unilamellar vesicles of conventional (LUV VAN), fusogenic (LUV(fuso) VAN), and cationic (LU...

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Autores principales: Scriboni, Andreia Borges, Couto, Verônica Muniz, Ribeiro, Lígia Nunes de Morais, Freires, Irlan Almeida, Groppo, Francisco Carlos, de Paula, Eneida, Franz-Montan, Michelle, Cogo-Müller, Karina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895244/
https://www.ncbi.nlm.nih.gov/pubmed/31849660
http://dx.doi.org/10.3389/fphar.2019.01401
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author Scriboni, Andreia Borges
Couto, Verônica Muniz
Ribeiro, Lígia Nunes de Morais
Freires, Irlan Almeida
Groppo, Francisco Carlos
de Paula, Eneida
Franz-Montan, Michelle
Cogo-Müller, Karina
author_facet Scriboni, Andreia Borges
Couto, Verônica Muniz
Ribeiro, Lígia Nunes de Morais
Freires, Irlan Almeida
Groppo, Francisco Carlos
de Paula, Eneida
Franz-Montan, Michelle
Cogo-Müller, Karina
author_sort Scriboni, Andreia Borges
collection PubMed
description Objective: The aim of the present study was to encapsulate vancomycin in different liposomal formulations and compare the in vitro antimicrobial activity against Staphylococcus aureus biofilms. Methods: Large unilamellar vesicles of conventional (LUV VAN), fusogenic (LUV(fuso) VAN), and cationic (LUV(cat) VAN) liposomes encapsulating VAN were characterized in terms of size, polydispersity index, zeta potential, morphology, encapsulation efficiency (%EE) and in vitro release kinetics. The formulations were tested for their Minimum Inhibitory Concentration (MIC) and inhibitory activity on biofilm formation and viability, using methicillin-susceptible S. aureus ATCC 29213 and methicillin-resistant S. aureus ATCC 43300 strains. Key Findings: LUV VAN showed better %EE (32.5%) and sustained release than LUV(fuso) VAN, LUV(cat) VAN, and free VAN. The formulations were stable over 180 days at 4°C, except for LUV VAN, which was stable up to 120 days. The MIC values for liposomal formulations and free VAN ranged from 0.78 to 1.56 µg/ml against both tested strains, with no difference in the inhibition of biofilm formation as compared to free VAN. However, when treating mature biofilm, encapsulated LUV(fuso) VAN increased the antimicrobial efficacy as compared to the other liposomal formulations and to free VAN, demonstrating a better ability to penetrate the biofilm. Conclusion: Vancomycin encapsulated in fusogenic liposomes demonstrated enhanced antimicrobial activity against mature S. aureus biofilms.
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spelling pubmed-68952442019-12-17 Fusogenic Liposomes Increase the Antimicrobial Activity of Vancomycin Against Staphylococcus aureus Biofilm Scriboni, Andreia Borges Couto, Verônica Muniz Ribeiro, Lígia Nunes de Morais Freires, Irlan Almeida Groppo, Francisco Carlos de Paula, Eneida Franz-Montan, Michelle Cogo-Müller, Karina Front Pharmacol Pharmacology Objective: The aim of the present study was to encapsulate vancomycin in different liposomal formulations and compare the in vitro antimicrobial activity against Staphylococcus aureus biofilms. Methods: Large unilamellar vesicles of conventional (LUV VAN), fusogenic (LUV(fuso) VAN), and cationic (LUV(cat) VAN) liposomes encapsulating VAN were characterized in terms of size, polydispersity index, zeta potential, morphology, encapsulation efficiency (%EE) and in vitro release kinetics. The formulations were tested for their Minimum Inhibitory Concentration (MIC) and inhibitory activity on biofilm formation and viability, using methicillin-susceptible S. aureus ATCC 29213 and methicillin-resistant S. aureus ATCC 43300 strains. Key Findings: LUV VAN showed better %EE (32.5%) and sustained release than LUV(fuso) VAN, LUV(cat) VAN, and free VAN. The formulations were stable over 180 days at 4°C, except for LUV VAN, which was stable up to 120 days. The MIC values for liposomal formulations and free VAN ranged from 0.78 to 1.56 µg/ml against both tested strains, with no difference in the inhibition of biofilm formation as compared to free VAN. However, when treating mature biofilm, encapsulated LUV(fuso) VAN increased the antimicrobial efficacy as compared to the other liposomal formulations and to free VAN, demonstrating a better ability to penetrate the biofilm. Conclusion: Vancomycin encapsulated in fusogenic liposomes demonstrated enhanced antimicrobial activity against mature S. aureus biofilms. Frontiers Media S.A. 2019-11-29 /pmc/articles/PMC6895244/ /pubmed/31849660 http://dx.doi.org/10.3389/fphar.2019.01401 Text en Copyright © 2019 Scriboni, Couto, Ribeiro, Freires, Groppo, de Paula, Franz-Montan and Cogo-Müller http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Scriboni, Andreia Borges
Couto, Verônica Muniz
Ribeiro, Lígia Nunes de Morais
Freires, Irlan Almeida
Groppo, Francisco Carlos
de Paula, Eneida
Franz-Montan, Michelle
Cogo-Müller, Karina
Fusogenic Liposomes Increase the Antimicrobial Activity of Vancomycin Against Staphylococcus aureus Biofilm
title Fusogenic Liposomes Increase the Antimicrobial Activity of Vancomycin Against Staphylococcus aureus Biofilm
title_full Fusogenic Liposomes Increase the Antimicrobial Activity of Vancomycin Against Staphylococcus aureus Biofilm
title_fullStr Fusogenic Liposomes Increase the Antimicrobial Activity of Vancomycin Against Staphylococcus aureus Biofilm
title_full_unstemmed Fusogenic Liposomes Increase the Antimicrobial Activity of Vancomycin Against Staphylococcus aureus Biofilm
title_short Fusogenic Liposomes Increase the Antimicrobial Activity of Vancomycin Against Staphylococcus aureus Biofilm
title_sort fusogenic liposomes increase the antimicrobial activity of vancomycin against staphylococcus aureus biofilm
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895244/
https://www.ncbi.nlm.nih.gov/pubmed/31849660
http://dx.doi.org/10.3389/fphar.2019.01401
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