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Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave
In fission yeast, MBF-dependent transcription is inactivated at the end of S phase through a negative feedback loop that involves the co-repressors, Yox1 and Nrm1. Although this repression system is well known, the molecular mechanisms involved in MBF activation remain largely unknown. Compacted chr...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895280/ https://www.ncbi.nlm.nih.gov/pubmed/31260531 http://dx.doi.org/10.1093/nar/gkz561 |
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author | González-Medina, Alberto Hidalgo, Elena Ayté, José |
author_facet | González-Medina, Alberto Hidalgo, Elena Ayté, José |
author_sort | González-Medina, Alberto |
collection | PubMed |
description | In fission yeast, MBF-dependent transcription is inactivated at the end of S phase through a negative feedback loop that involves the co-repressors, Yox1 and Nrm1. Although this repression system is well known, the molecular mechanisms involved in MBF activation remain largely unknown. Compacted chromatin constitutes a barrier to activators accessing promoters. Here, we show that chromatin regulation plays a key role in activating MBF-dependent transcription. Gcn5, a part of the SAGA complex, binds to MBF-regulated promoters through the MBF co-activator Rep2 in a cell cycle-dependent manner and in a reverse correlation to the binding of the MBF co-repressors, Nrm1 or Yox1. We propose that the co-repressors function as physical barriers to SAGA recruitment onto MBF promoters. We also show that Gcn5 acetylates specific lysine residues on histone H3 in a cell cycle-regulated manner. Furthermore, either in a gcn5 mutant or in a strain in which histone H3 is kept in an unacetylated form, MBF-dependent transcription is downregulated. In summary, Gcn5 is required for the full activation and correct timing of MBF-regulated gene transcription. |
format | Online Article Text |
id | pubmed-6895280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-68952802019-12-11 Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave González-Medina, Alberto Hidalgo, Elena Ayté, José Nucleic Acids Res Gene regulation, Chromatin and Epigenetics In fission yeast, MBF-dependent transcription is inactivated at the end of S phase through a negative feedback loop that involves the co-repressors, Yox1 and Nrm1. Although this repression system is well known, the molecular mechanisms involved in MBF activation remain largely unknown. Compacted chromatin constitutes a barrier to activators accessing promoters. Here, we show that chromatin regulation plays a key role in activating MBF-dependent transcription. Gcn5, a part of the SAGA complex, binds to MBF-regulated promoters through the MBF co-activator Rep2 in a cell cycle-dependent manner and in a reverse correlation to the binding of the MBF co-repressors, Nrm1 or Yox1. We propose that the co-repressors function as physical barriers to SAGA recruitment onto MBF promoters. We also show that Gcn5 acetylates specific lysine residues on histone H3 in a cell cycle-regulated manner. Furthermore, either in a gcn5 mutant or in a strain in which histone H3 is kept in an unacetylated form, MBF-dependent transcription is downregulated. In summary, Gcn5 is required for the full activation and correct timing of MBF-regulated gene transcription. Oxford University Press 2019-09-19 2019-07-01 /pmc/articles/PMC6895280/ /pubmed/31260531 http://dx.doi.org/10.1093/nar/gkz561 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Gene regulation, Chromatin and Epigenetics González-Medina, Alberto Hidalgo, Elena Ayté, José Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave |
title | Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave |
title_full | Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave |
title_fullStr | Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave |
title_full_unstemmed | Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave |
title_short | Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave |
title_sort | gcn5-mediated acetylation at mbf-regulated promoters induces the g1/s transcriptional wave |
topic | Gene regulation, Chromatin and Epigenetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895280/ https://www.ncbi.nlm.nih.gov/pubmed/31260531 http://dx.doi.org/10.1093/nar/gkz561 |
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