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Isolation and identification of cholestane and dihydropyrene from Calophyllum inophyllum

The highest population of Calophyllum inophyllum is in Indonesia. One of the bioactive compound contained in C. inophyllum leave is cholestane. The extraction method was employed to obtain crude extract from these leaves. It was followed by identification of the bioactive compounds. The purpose of t...

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Autores principales: Susanto, David Febrilliant, Aparamarta, Hakun Wirawasista, Widjaja, Arief, Jadid, Nurul, Gunawan, Setiyo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895651/
https://www.ncbi.nlm.nih.gov/pubmed/31844759
http://dx.doi.org/10.1016/j.heliyon.2019.e02893
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author Susanto, David Febrilliant
Aparamarta, Hakun Wirawasista
Widjaja, Arief
Jadid, Nurul
Gunawan, Setiyo
author_facet Susanto, David Febrilliant
Aparamarta, Hakun Wirawasista
Widjaja, Arief
Jadid, Nurul
Gunawan, Setiyo
author_sort Susanto, David Febrilliant
collection PubMed
description The highest population of Calophyllum inophyllum is in Indonesia. One of the bioactive compound contained in C. inophyllum leave is cholestane. The extraction method was employed to obtain crude extract from these leaves. It was followed by identification of the bioactive compounds. The purpose of this research was to develop a process for isolating and identifying cholestane and dihydropyrene from methanolic extract of C. inophyllum leaves in high yield and purity. The effect of crude extract to non-polar solvent mass ratio and non-polar solvent types on the separation of the bioactive compounds were also systematically investigated. New compounds (trans-2-[2-(trifluoromethyl)phenyl]-10b,10c-dimethyl-10b, 10c-dihydropyrene and anti-4-aza-B-homo-5.alpha-cholestane-3-one) were also identified in C. inophyllum leaves. The successful separation was obtained by employing CS(2) as the solvent and crude extract to CS(2) mass ratio of 1/10 (g/g).
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spelling pubmed-68956512019-12-16 Isolation and identification of cholestane and dihydropyrene from Calophyllum inophyllum Susanto, David Febrilliant Aparamarta, Hakun Wirawasista Widjaja, Arief Jadid, Nurul Gunawan, Setiyo Heliyon Article The highest population of Calophyllum inophyllum is in Indonesia. One of the bioactive compound contained in C. inophyllum leave is cholestane. The extraction method was employed to obtain crude extract from these leaves. It was followed by identification of the bioactive compounds. The purpose of this research was to develop a process for isolating and identifying cholestane and dihydropyrene from methanolic extract of C. inophyllum leaves in high yield and purity. The effect of crude extract to non-polar solvent mass ratio and non-polar solvent types on the separation of the bioactive compounds were also systematically investigated. New compounds (trans-2-[2-(trifluoromethyl)phenyl]-10b,10c-dimethyl-10b, 10c-dihydropyrene and anti-4-aza-B-homo-5.alpha-cholestane-3-one) were also identified in C. inophyllum leaves. The successful separation was obtained by employing CS(2) as the solvent and crude extract to CS(2) mass ratio of 1/10 (g/g). Elsevier 2019-11-21 /pmc/articles/PMC6895651/ /pubmed/31844759 http://dx.doi.org/10.1016/j.heliyon.2019.e02893 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Susanto, David Febrilliant
Aparamarta, Hakun Wirawasista
Widjaja, Arief
Jadid, Nurul
Gunawan, Setiyo
Isolation and identification of cholestane and dihydropyrene from Calophyllum inophyllum
title Isolation and identification of cholestane and dihydropyrene from Calophyllum inophyllum
title_full Isolation and identification of cholestane and dihydropyrene from Calophyllum inophyllum
title_fullStr Isolation and identification of cholestane and dihydropyrene from Calophyllum inophyllum
title_full_unstemmed Isolation and identification of cholestane and dihydropyrene from Calophyllum inophyllum
title_short Isolation and identification of cholestane and dihydropyrene from Calophyllum inophyllum
title_sort isolation and identification of cholestane and dihydropyrene from calophyllum inophyllum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895651/
https://www.ncbi.nlm.nih.gov/pubmed/31844759
http://dx.doi.org/10.1016/j.heliyon.2019.e02893
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