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Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules

Females across many internally fertilizing taxa store sperm, often in specialized storage organs in their reproductive tracts. In birds, several hundred sperm storage tubules exist in the utero-vaginal junction of the oviduct, and there is growing evidence that sperm storage in these tubules is sele...

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Detalles Bibliográficos
Autores principales: Mendonca, Tania, Cadby, Ashley J., Hemmings, Nicola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895713/
https://www.ncbi.nlm.nih.gov/pubmed/31733725
http://dx.doi.org/10.1016/j.bpj.2019.10.038
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author Mendonca, Tania
Cadby, Ashley J.
Hemmings, Nicola
author_facet Mendonca, Tania
Cadby, Ashley J.
Hemmings, Nicola
author_sort Mendonca, Tania
collection PubMed
description Females across many internally fertilizing taxa store sperm, often in specialized storage organs in their reproductive tracts. In birds, several hundred sperm storage tubules exist in the utero-vaginal junction of the oviduct, and there is growing evidence that sperm storage in these tubules is selective. The mechanisms underlying female sperm storage in birds remain unknown because of our limited ability to make three-dimensional, live observations inside the large, muscular avian oviduct. Here, we describe a new application of fluorescence selective plane illumination microscopy to optically section oviduct tissue from zebra finch Taeniopygia guttata females label free by harnessing tissue autofluorescence. Our data provide the first description of the three-dimensional structure of sperm storage organs in any vertebrate to the best of our knowledge and reveal the presence of gate-like constricted openings that may play a role in sperm selection.
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spelling pubmed-68957132020-10-10 Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules Mendonca, Tania Cadby, Ashley J. Hemmings, Nicola Biophys J Articles Females across many internally fertilizing taxa store sperm, often in specialized storage organs in their reproductive tracts. In birds, several hundred sperm storage tubules exist in the utero-vaginal junction of the oviduct, and there is growing evidence that sperm storage in these tubules is selective. The mechanisms underlying female sperm storage in birds remain unknown because of our limited ability to make three-dimensional, live observations inside the large, muscular avian oviduct. Here, we describe a new application of fluorescence selective plane illumination microscopy to optically section oviduct tissue from zebra finch Taeniopygia guttata females label free by harnessing tissue autofluorescence. Our data provide the first description of the three-dimensional structure of sperm storage organs in any vertebrate to the best of our knowledge and reveal the presence of gate-like constricted openings that may play a role in sperm selection. The Biophysical Society 2019-12-03 2019-11-05 /pmc/articles/PMC6895713/ /pubmed/31733725 http://dx.doi.org/10.1016/j.bpj.2019.10.038 Text en © 2019 Biophysical Society. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Articles
Mendonca, Tania
Cadby, Ashley J.
Hemmings, Nicola
Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules
title Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules
title_full Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules
title_fullStr Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules
title_full_unstemmed Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules
title_short Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules
title_sort sperm gatekeeping: 3d imaging reveals a constricted entrance to zebra finch sperm storage tubules
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895713/
https://www.ncbi.nlm.nih.gov/pubmed/31733725
http://dx.doi.org/10.1016/j.bpj.2019.10.038
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