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Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules
Females across many internally fertilizing taxa store sperm, often in specialized storage organs in their reproductive tracts. In birds, several hundred sperm storage tubules exist in the utero-vaginal junction of the oviduct, and there is growing evidence that sperm storage in these tubules is sele...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Biophysical Society
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895713/ https://www.ncbi.nlm.nih.gov/pubmed/31733725 http://dx.doi.org/10.1016/j.bpj.2019.10.038 |
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author | Mendonca, Tania Cadby, Ashley J. Hemmings, Nicola |
author_facet | Mendonca, Tania Cadby, Ashley J. Hemmings, Nicola |
author_sort | Mendonca, Tania |
collection | PubMed |
description | Females across many internally fertilizing taxa store sperm, often in specialized storage organs in their reproductive tracts. In birds, several hundred sperm storage tubules exist in the utero-vaginal junction of the oviduct, and there is growing evidence that sperm storage in these tubules is selective. The mechanisms underlying female sperm storage in birds remain unknown because of our limited ability to make three-dimensional, live observations inside the large, muscular avian oviduct. Here, we describe a new application of fluorescence selective plane illumination microscopy to optically section oviduct tissue from zebra finch Taeniopygia guttata females label free by harnessing tissue autofluorescence. Our data provide the first description of the three-dimensional structure of sperm storage organs in any vertebrate to the best of our knowledge and reveal the presence of gate-like constricted openings that may play a role in sperm selection. |
format | Online Article Text |
id | pubmed-6895713 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Biophysical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-68957132020-10-10 Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules Mendonca, Tania Cadby, Ashley J. Hemmings, Nicola Biophys J Articles Females across many internally fertilizing taxa store sperm, often in specialized storage organs in their reproductive tracts. In birds, several hundred sperm storage tubules exist in the utero-vaginal junction of the oviduct, and there is growing evidence that sperm storage in these tubules is selective. The mechanisms underlying female sperm storage in birds remain unknown because of our limited ability to make three-dimensional, live observations inside the large, muscular avian oviduct. Here, we describe a new application of fluorescence selective plane illumination microscopy to optically section oviduct tissue from zebra finch Taeniopygia guttata females label free by harnessing tissue autofluorescence. Our data provide the first description of the three-dimensional structure of sperm storage organs in any vertebrate to the best of our knowledge and reveal the presence of gate-like constricted openings that may play a role in sperm selection. The Biophysical Society 2019-12-03 2019-11-05 /pmc/articles/PMC6895713/ /pubmed/31733725 http://dx.doi.org/10.1016/j.bpj.2019.10.038 Text en © 2019 Biophysical Society. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Articles Mendonca, Tania Cadby, Ashley J. Hemmings, Nicola Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules |
title | Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules |
title_full | Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules |
title_fullStr | Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules |
title_full_unstemmed | Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules |
title_short | Sperm Gatekeeping: 3D Imaging Reveals a Constricted Entrance to Zebra Finch Sperm Storage Tubules |
title_sort | sperm gatekeeping: 3d imaging reveals a constricted entrance to zebra finch sperm storage tubules |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6895713/ https://www.ncbi.nlm.nih.gov/pubmed/31733725 http://dx.doi.org/10.1016/j.bpj.2019.10.038 |
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