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Genome-Wide Analysis of Members of the WRKY Gene Family and Their Cold Stress Response in Prunus mume

Prunus mume, which is a rosaceous arbor with very high ornamental, edible and medical values, has a distribution that is mainly restricted by low temperature. WRKY transcription factor genes play crucial roles in the growth, development, and stress responses of plants. However, the WRKY gene family...

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Autores principales: Bao, Fei, Ding, Anqi, Cheng, Tangren, Wang, Jia, Zhang, Qixiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896039/
https://www.ncbi.nlm.nih.gov/pubmed/31717396
http://dx.doi.org/10.3390/genes10110911
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author Bao, Fei
Ding, Anqi
Cheng, Tangren
Wang, Jia
Zhang, Qixiang
author_facet Bao, Fei
Ding, Anqi
Cheng, Tangren
Wang, Jia
Zhang, Qixiang
author_sort Bao, Fei
collection PubMed
description Prunus mume, which is a rosaceous arbor with very high ornamental, edible and medical values, has a distribution that is mainly restricted by low temperature. WRKY transcription factor genes play crucial roles in the growth, development, and stress responses of plants. However, the WRKY gene family has not been characterised in P. mume. There were 58 PmWRKYs identified from genome of P. mume. They were anchored onto eight link groups and categorised into three broad groups. The gene structure and motif composition were reasonably conservative in each group. Investigation of gene duplication indicated that nine and seven PmWRKYs were arranged in tandem and segmental duplications, respectively. PmWRKYs were discriminately expressed in different tissues (i.e., roots, stems, leaves, flowers and fruits) in P. mume. The 17 cold-related candidate genes were selected based on RNA-seq data. Further, to investigate the function of PmWRKYs in low temperatures, the expression patterns under artificial cold treatments were analysed. The results showed that the expression levels of the 12 PmWRKYs genes significantly and 5 genes slightly changed in stems. In particular, the expression level of PmWRKY18 was up-regulated after ABA treatment. In addition, the spatiotemporal expression patterns of 17 PmWRKYs were analysed in winter. These results indicated that 17 PmWRKYs were potential transcription factors regulating cold resistance in P. mume.
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spelling pubmed-68960392019-12-23 Genome-Wide Analysis of Members of the WRKY Gene Family and Their Cold Stress Response in Prunus mume Bao, Fei Ding, Anqi Cheng, Tangren Wang, Jia Zhang, Qixiang Genes (Basel) Article Prunus mume, which is a rosaceous arbor with very high ornamental, edible and medical values, has a distribution that is mainly restricted by low temperature. WRKY transcription factor genes play crucial roles in the growth, development, and stress responses of plants. However, the WRKY gene family has not been characterised in P. mume. There were 58 PmWRKYs identified from genome of P. mume. They were anchored onto eight link groups and categorised into three broad groups. The gene structure and motif composition were reasonably conservative in each group. Investigation of gene duplication indicated that nine and seven PmWRKYs were arranged in tandem and segmental duplications, respectively. PmWRKYs were discriminately expressed in different tissues (i.e., roots, stems, leaves, flowers and fruits) in P. mume. The 17 cold-related candidate genes were selected based on RNA-seq data. Further, to investigate the function of PmWRKYs in low temperatures, the expression patterns under artificial cold treatments were analysed. The results showed that the expression levels of the 12 PmWRKYs genes significantly and 5 genes slightly changed in stems. In particular, the expression level of PmWRKY18 was up-regulated after ABA treatment. In addition, the spatiotemporal expression patterns of 17 PmWRKYs were analysed in winter. These results indicated that 17 PmWRKYs were potential transcription factors regulating cold resistance in P. mume. MDPI 2019-11-08 /pmc/articles/PMC6896039/ /pubmed/31717396 http://dx.doi.org/10.3390/genes10110911 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bao, Fei
Ding, Anqi
Cheng, Tangren
Wang, Jia
Zhang, Qixiang
Genome-Wide Analysis of Members of the WRKY Gene Family and Their Cold Stress Response in Prunus mume
title Genome-Wide Analysis of Members of the WRKY Gene Family and Their Cold Stress Response in Prunus mume
title_full Genome-Wide Analysis of Members of the WRKY Gene Family and Their Cold Stress Response in Prunus mume
title_fullStr Genome-Wide Analysis of Members of the WRKY Gene Family and Their Cold Stress Response in Prunus mume
title_full_unstemmed Genome-Wide Analysis of Members of the WRKY Gene Family and Their Cold Stress Response in Prunus mume
title_short Genome-Wide Analysis of Members of the WRKY Gene Family and Their Cold Stress Response in Prunus mume
title_sort genome-wide analysis of members of the wrky gene family and their cold stress response in prunus mume
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896039/
https://www.ncbi.nlm.nih.gov/pubmed/31717396
http://dx.doi.org/10.3390/genes10110911
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