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Synergistic Antitumorigenic Activity of Calcitriol with Curcumin or Resveratrol is Mediated by Angiogenesis Inhibition in Triple Negative Breast Cancer Xenografts
Calcitriol is a multitarget anticancer hormone; however, its effects on angiogenesis remain contradictory. Herein, we tested whether the antiangiogenic phytochemicals curcumin or resveratrol improved calcitriol antitumorigenic effects in vivo. Triple-negative breast cancer tumoral cells (MBCDF-T) we...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896056/ https://www.ncbi.nlm.nih.gov/pubmed/31698751 http://dx.doi.org/10.3390/cancers11111739 |
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author | García-Quiroz, Janice García-Becerra, Rocío Santos-Cuevas, Clara Ramírez-Nava, Gerardo J. Morales-Guadarrama, Gabriela Cárdenas-Ochoa, Nohemí Segovia-Mendoza, Mariana Prado-Garcia, Heriberto Ordaz-Rosado, David Avila, Euclides Olmos-Ortiz, Andrea López-Cisneros, Sofía Larrea, Fernando Díaz, Lorenza |
author_facet | García-Quiroz, Janice García-Becerra, Rocío Santos-Cuevas, Clara Ramírez-Nava, Gerardo J. Morales-Guadarrama, Gabriela Cárdenas-Ochoa, Nohemí Segovia-Mendoza, Mariana Prado-Garcia, Heriberto Ordaz-Rosado, David Avila, Euclides Olmos-Ortiz, Andrea López-Cisneros, Sofía Larrea, Fernando Díaz, Lorenza |
author_sort | García-Quiroz, Janice |
collection | PubMed |
description | Calcitriol is a multitarget anticancer hormone; however, its effects on angiogenesis remain contradictory. Herein, we tested whether the antiangiogenic phytochemicals curcumin or resveratrol improved calcitriol antitumorigenic effects in vivo. Triple-negative breast cancer tumoral cells (MBCDF-T) were xenografted in nude mice, maintaining treatments for 3 weeks. Tumor onset, volume and microvessel density were significantly reduced in mice coadministered with calcitriol and curcumin (Cal+Cur). Vessel count was also reduced in mice simultaneously treated with calcitriol and resveratrol (Cal+Rsv). Cal+Cur and Cal+Rsv treatments resulted in less tumor activated endothelium, as demonstrated by decreased tumor uptake of integrin-targeted biosensors in vivo. The renal gene expression of Cyp24a1 and Cyp27b1 suggested increased calcitriol bioactivity in the combined regimens. In vitro, the phytochemicals inhibited both MBCDF-T and endothelial cells proliferation, while potentiated calcitriol’s ability to reduce MBCDF-T cell-growth and endothelial cells migration. Resveratrol induced endothelial cell death, as deduced by increased sub-G1 cells accumulation, explaining the reduced tumor vessel number in resveratrol-treated mice, which further diminished when combined with calcitriol. In conclusion, the concomitant administration of calcitriol with curcumin or resveratrol synergistically promoted anticancer effects in vitro and in vivo in human mammary tumor cells. Whereas the results suggest different mechanisms of action of the phytochemicals when coadministered with calcitriol, the converging biological effect was inhibition of tumor neoangiogenesis. |
format | Online Article Text |
id | pubmed-6896056 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68960562019-12-23 Synergistic Antitumorigenic Activity of Calcitriol with Curcumin or Resveratrol is Mediated by Angiogenesis Inhibition in Triple Negative Breast Cancer Xenografts García-Quiroz, Janice García-Becerra, Rocío Santos-Cuevas, Clara Ramírez-Nava, Gerardo J. Morales-Guadarrama, Gabriela Cárdenas-Ochoa, Nohemí Segovia-Mendoza, Mariana Prado-Garcia, Heriberto Ordaz-Rosado, David Avila, Euclides Olmos-Ortiz, Andrea López-Cisneros, Sofía Larrea, Fernando Díaz, Lorenza Cancers (Basel) Article Calcitriol is a multitarget anticancer hormone; however, its effects on angiogenesis remain contradictory. Herein, we tested whether the antiangiogenic phytochemicals curcumin or resveratrol improved calcitriol antitumorigenic effects in vivo. Triple-negative breast cancer tumoral cells (MBCDF-T) were xenografted in nude mice, maintaining treatments for 3 weeks. Tumor onset, volume and microvessel density were significantly reduced in mice coadministered with calcitriol and curcumin (Cal+Cur). Vessel count was also reduced in mice simultaneously treated with calcitriol and resveratrol (Cal+Rsv). Cal+Cur and Cal+Rsv treatments resulted in less tumor activated endothelium, as demonstrated by decreased tumor uptake of integrin-targeted biosensors in vivo. The renal gene expression of Cyp24a1 and Cyp27b1 suggested increased calcitriol bioactivity in the combined regimens. In vitro, the phytochemicals inhibited both MBCDF-T and endothelial cells proliferation, while potentiated calcitriol’s ability to reduce MBCDF-T cell-growth and endothelial cells migration. Resveratrol induced endothelial cell death, as deduced by increased sub-G1 cells accumulation, explaining the reduced tumor vessel number in resveratrol-treated mice, which further diminished when combined with calcitriol. In conclusion, the concomitant administration of calcitriol with curcumin or resveratrol synergistically promoted anticancer effects in vitro and in vivo in human mammary tumor cells. Whereas the results suggest different mechanisms of action of the phytochemicals when coadministered with calcitriol, the converging biological effect was inhibition of tumor neoangiogenesis. MDPI 2019-11-06 /pmc/articles/PMC6896056/ /pubmed/31698751 http://dx.doi.org/10.3390/cancers11111739 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article García-Quiroz, Janice García-Becerra, Rocío Santos-Cuevas, Clara Ramírez-Nava, Gerardo J. Morales-Guadarrama, Gabriela Cárdenas-Ochoa, Nohemí Segovia-Mendoza, Mariana Prado-Garcia, Heriberto Ordaz-Rosado, David Avila, Euclides Olmos-Ortiz, Andrea López-Cisneros, Sofía Larrea, Fernando Díaz, Lorenza Synergistic Antitumorigenic Activity of Calcitriol with Curcumin or Resveratrol is Mediated by Angiogenesis Inhibition in Triple Negative Breast Cancer Xenografts |
title | Synergistic Antitumorigenic Activity of Calcitriol with Curcumin or Resveratrol is Mediated by Angiogenesis Inhibition in Triple Negative Breast Cancer Xenografts |
title_full | Synergistic Antitumorigenic Activity of Calcitriol with Curcumin or Resveratrol is Mediated by Angiogenesis Inhibition in Triple Negative Breast Cancer Xenografts |
title_fullStr | Synergistic Antitumorigenic Activity of Calcitriol with Curcumin or Resveratrol is Mediated by Angiogenesis Inhibition in Triple Negative Breast Cancer Xenografts |
title_full_unstemmed | Synergistic Antitumorigenic Activity of Calcitriol with Curcumin or Resveratrol is Mediated by Angiogenesis Inhibition in Triple Negative Breast Cancer Xenografts |
title_short | Synergistic Antitumorigenic Activity of Calcitriol with Curcumin or Resveratrol is Mediated by Angiogenesis Inhibition in Triple Negative Breast Cancer Xenografts |
title_sort | synergistic antitumorigenic activity of calcitriol with curcumin or resveratrol is mediated by angiogenesis inhibition in triple negative breast cancer xenografts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896056/ https://www.ncbi.nlm.nih.gov/pubmed/31698751 http://dx.doi.org/10.3390/cancers11111739 |
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