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MicroRNA-1258 Inhibits the Proliferation and Migration of Human Colorectal Cancer Cells through Suppressing CKS1B Expression

Increasing evidence has demonstrated that increased expression of cyclin-dependent kinase regulatory subunit 1B (CKS1B) is associated with the pathogenesis of many human cancers, including colorectal cancer (CRC). However, the regulatory mechanisms underlying the expression of CKS1B in CRC are not c...

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Autores principales: Hwang, Jin-Seong, Jeong, Eun-Jeong, Choi, Jinhyeon, Lee, Yeo-Jin, Jung, Eunsun, Kim, Seon-Kyu, Min, Jeong-Ki, Han, Tae-Su, Kim, Jang-Seong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896137/
https://www.ncbi.nlm.nih.gov/pubmed/31717435
http://dx.doi.org/10.3390/genes10110912
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author Hwang, Jin-Seong
Jeong, Eun-Jeong
Choi, Jinhyeon
Lee, Yeo-Jin
Jung, Eunsun
Kim, Seon-Kyu
Min, Jeong-Ki
Han, Tae-Su
Kim, Jang-Seong
author_facet Hwang, Jin-Seong
Jeong, Eun-Jeong
Choi, Jinhyeon
Lee, Yeo-Jin
Jung, Eunsun
Kim, Seon-Kyu
Min, Jeong-Ki
Han, Tae-Su
Kim, Jang-Seong
author_sort Hwang, Jin-Seong
collection PubMed
description Increasing evidence has demonstrated that increased expression of cyclin-dependent kinase regulatory subunit 1B (CKS1B) is associated with the pathogenesis of many human cancers, including colorectal cancer (CRC). However, the regulatory mechanisms underlying the expression of CKS1B in CRC are not completely understood. Here, we investigate the role played by microRNAs in the expression of CKS1B and carcinogenesis in CRC. Among the six microRNAs predicted to target CKS1B gene expression, only miR-1258 was revealed to downregulate CKS1B expression through binding to its 3’-UTR region, as ectopic miR-1258 expression suppressed CKS1B expression and vice versa. In CRC, miR-1258 expression also decreased cell proliferation and migration in vitro and tumor growth in vivo, similar to cells with silenced CKS1B expression. Considering the highly increased levels of CKS1B and decreased expression of miR-1258 in tumors from CRC patients, these findings suggest that miR-1258 may play tumor-suppressive roles by targeting CKS1B expression in CRC. However, the therapeutic significance of these findings should be evaluated in clinical settings.
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spelling pubmed-68961372019-12-23 MicroRNA-1258 Inhibits the Proliferation and Migration of Human Colorectal Cancer Cells through Suppressing CKS1B Expression Hwang, Jin-Seong Jeong, Eun-Jeong Choi, Jinhyeon Lee, Yeo-Jin Jung, Eunsun Kim, Seon-Kyu Min, Jeong-Ki Han, Tae-Su Kim, Jang-Seong Genes (Basel) Article Increasing evidence has demonstrated that increased expression of cyclin-dependent kinase regulatory subunit 1B (CKS1B) is associated with the pathogenesis of many human cancers, including colorectal cancer (CRC). However, the regulatory mechanisms underlying the expression of CKS1B in CRC are not completely understood. Here, we investigate the role played by microRNAs in the expression of CKS1B and carcinogenesis in CRC. Among the six microRNAs predicted to target CKS1B gene expression, only miR-1258 was revealed to downregulate CKS1B expression through binding to its 3’-UTR region, as ectopic miR-1258 expression suppressed CKS1B expression and vice versa. In CRC, miR-1258 expression also decreased cell proliferation and migration in vitro and tumor growth in vivo, similar to cells with silenced CKS1B expression. Considering the highly increased levels of CKS1B and decreased expression of miR-1258 in tumors from CRC patients, these findings suggest that miR-1258 may play tumor-suppressive roles by targeting CKS1B expression in CRC. However, the therapeutic significance of these findings should be evaluated in clinical settings. MDPI 2019-11-08 /pmc/articles/PMC6896137/ /pubmed/31717435 http://dx.doi.org/10.3390/genes10110912 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hwang, Jin-Seong
Jeong, Eun-Jeong
Choi, Jinhyeon
Lee, Yeo-Jin
Jung, Eunsun
Kim, Seon-Kyu
Min, Jeong-Ki
Han, Tae-Su
Kim, Jang-Seong
MicroRNA-1258 Inhibits the Proliferation and Migration of Human Colorectal Cancer Cells through Suppressing CKS1B Expression
title MicroRNA-1258 Inhibits the Proliferation and Migration of Human Colorectal Cancer Cells through Suppressing CKS1B Expression
title_full MicroRNA-1258 Inhibits the Proliferation and Migration of Human Colorectal Cancer Cells through Suppressing CKS1B Expression
title_fullStr MicroRNA-1258 Inhibits the Proliferation and Migration of Human Colorectal Cancer Cells through Suppressing CKS1B Expression
title_full_unstemmed MicroRNA-1258 Inhibits the Proliferation and Migration of Human Colorectal Cancer Cells through Suppressing CKS1B Expression
title_short MicroRNA-1258 Inhibits the Proliferation and Migration of Human Colorectal Cancer Cells through Suppressing CKS1B Expression
title_sort microrna-1258 inhibits the proliferation and migration of human colorectal cancer cells through suppressing cks1b expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896137/
https://www.ncbi.nlm.nih.gov/pubmed/31717435
http://dx.doi.org/10.3390/genes10110912
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