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Endoplasmic reticulum stress regulates epithelial-mesenchymal transition in human lens epithelial cells

Epithelial-to-mesenchymal transition (EMT) of human lens epithelial cells (HLECs) serve an important role in cataract formation. The endoplasmic reticulum stress response (ER stress) has been demonstrated to regulate EMT in a number of tissues. The aim of the present study was to demonstrate the rol...

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Autores principales: Zhou, Sheng, Yang, Jing, Wang, Mingwei, Zheng, Danying, Liu, Yizhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896292/
https://www.ncbi.nlm.nih.gov/pubmed/31746423
http://dx.doi.org/10.3892/mmr.2019.10814
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author Zhou, Sheng
Yang, Jing
Wang, Mingwei
Zheng, Danying
Liu, Yizhi
author_facet Zhou, Sheng
Yang, Jing
Wang, Mingwei
Zheng, Danying
Liu, Yizhi
author_sort Zhou, Sheng
collection PubMed
description Epithelial-to-mesenchymal transition (EMT) of human lens epithelial cells (HLECs) serve an important role in cataract formation. The endoplasmic reticulum stress response (ER stress) has been demonstrated to regulate EMT in a number of tissues. The aim of the present study was to demonstrate the role of ER stress on EMT in HLECs. HLECs were treated with tunicamycin (TM) or thapsigargin (TG) to disturb ER homeostasis, and 4-phenylbutyric acid (PBA) or sodium tauroursodeoxycholate (TUDCA) to restore ER homeostasis. Cell morphology was evaluated after 24 h. The long axis and aspect ratio of the cells were analyzed using ImageJ software. The results demonstrated that HLECs adopted an elongated morphology following treatment with TG, and the cellular aspect ratio increased. However, this morphological change was not observed following combination treatment with TG and PBA. Western blot analysis and immunofluorescence staining were used to measure the protein expression levels. A wound-healing assay was performed to evaluate cell migration. Treatment with TM or TG increased the expression of the ER stress markers glucose-regulated protein 78, phosphorylated eukaryotic initiation factor 2α, activating transcription factor (ATF)6, ATF4 and inositol-requiring protein 1α and the EMT markers fibronectin, vimentin, α-smooth muscle actin and neural cadherin. Furthermore, treatment with TM or TG decreased the expression of the epithelial cell marker epithelial cadherin and enhanced cell migration, which effects were inhibited following treatment with PBA or TUDCA. These results indicates that enhanced ER stress induced EMT and subsequently increased cell migration in HLECs in vitro.
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spelling pubmed-68962922019-12-09 Endoplasmic reticulum stress regulates epithelial-mesenchymal transition in human lens epithelial cells Zhou, Sheng Yang, Jing Wang, Mingwei Zheng, Danying Liu, Yizhi Mol Med Rep Articles Epithelial-to-mesenchymal transition (EMT) of human lens epithelial cells (HLECs) serve an important role in cataract formation. The endoplasmic reticulum stress response (ER stress) has been demonstrated to regulate EMT in a number of tissues. The aim of the present study was to demonstrate the role of ER stress on EMT in HLECs. HLECs were treated with tunicamycin (TM) or thapsigargin (TG) to disturb ER homeostasis, and 4-phenylbutyric acid (PBA) or sodium tauroursodeoxycholate (TUDCA) to restore ER homeostasis. Cell morphology was evaluated after 24 h. The long axis and aspect ratio of the cells were analyzed using ImageJ software. The results demonstrated that HLECs adopted an elongated morphology following treatment with TG, and the cellular aspect ratio increased. However, this morphological change was not observed following combination treatment with TG and PBA. Western blot analysis and immunofluorescence staining were used to measure the protein expression levels. A wound-healing assay was performed to evaluate cell migration. Treatment with TM or TG increased the expression of the ER stress markers glucose-regulated protein 78, phosphorylated eukaryotic initiation factor 2α, activating transcription factor (ATF)6, ATF4 and inositol-requiring protein 1α and the EMT markers fibronectin, vimentin, α-smooth muscle actin and neural cadherin. Furthermore, treatment with TM or TG decreased the expression of the epithelial cell marker epithelial cadherin and enhanced cell migration, which effects were inhibited following treatment with PBA or TUDCA. These results indicates that enhanced ER stress induced EMT and subsequently increased cell migration in HLECs in vitro. D.A. Spandidos 2020-01 2019-11-12 /pmc/articles/PMC6896292/ /pubmed/31746423 http://dx.doi.org/10.3892/mmr.2019.10814 Text en Copyright: © Zhou et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhou, Sheng
Yang, Jing
Wang, Mingwei
Zheng, Danying
Liu, Yizhi
Endoplasmic reticulum stress regulates epithelial-mesenchymal transition in human lens epithelial cells
title Endoplasmic reticulum stress regulates epithelial-mesenchymal transition in human lens epithelial cells
title_full Endoplasmic reticulum stress regulates epithelial-mesenchymal transition in human lens epithelial cells
title_fullStr Endoplasmic reticulum stress regulates epithelial-mesenchymal transition in human lens epithelial cells
title_full_unstemmed Endoplasmic reticulum stress regulates epithelial-mesenchymal transition in human lens epithelial cells
title_short Endoplasmic reticulum stress regulates epithelial-mesenchymal transition in human lens epithelial cells
title_sort endoplasmic reticulum stress regulates epithelial-mesenchymal transition in human lens epithelial cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896292/
https://www.ncbi.nlm.nih.gov/pubmed/31746423
http://dx.doi.org/10.3892/mmr.2019.10814
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