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Endoplasmic reticulum stress regulates epithelial-mesenchymal transition in human lens epithelial cells
Epithelial-to-mesenchymal transition (EMT) of human lens epithelial cells (HLECs) serve an important role in cataract formation. The endoplasmic reticulum stress response (ER stress) has been demonstrated to regulate EMT in a number of tissues. The aim of the present study was to demonstrate the rol...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896292/ https://www.ncbi.nlm.nih.gov/pubmed/31746423 http://dx.doi.org/10.3892/mmr.2019.10814 |
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author | Zhou, Sheng Yang, Jing Wang, Mingwei Zheng, Danying Liu, Yizhi |
author_facet | Zhou, Sheng Yang, Jing Wang, Mingwei Zheng, Danying Liu, Yizhi |
author_sort | Zhou, Sheng |
collection | PubMed |
description | Epithelial-to-mesenchymal transition (EMT) of human lens epithelial cells (HLECs) serve an important role in cataract formation. The endoplasmic reticulum stress response (ER stress) has been demonstrated to regulate EMT in a number of tissues. The aim of the present study was to demonstrate the role of ER stress on EMT in HLECs. HLECs were treated with tunicamycin (TM) or thapsigargin (TG) to disturb ER homeostasis, and 4-phenylbutyric acid (PBA) or sodium tauroursodeoxycholate (TUDCA) to restore ER homeostasis. Cell morphology was evaluated after 24 h. The long axis and aspect ratio of the cells were analyzed using ImageJ software. The results demonstrated that HLECs adopted an elongated morphology following treatment with TG, and the cellular aspect ratio increased. However, this morphological change was not observed following combination treatment with TG and PBA. Western blot analysis and immunofluorescence staining were used to measure the protein expression levels. A wound-healing assay was performed to evaluate cell migration. Treatment with TM or TG increased the expression of the ER stress markers glucose-regulated protein 78, phosphorylated eukaryotic initiation factor 2α, activating transcription factor (ATF)6, ATF4 and inositol-requiring protein 1α and the EMT markers fibronectin, vimentin, α-smooth muscle actin and neural cadherin. Furthermore, treatment with TM or TG decreased the expression of the epithelial cell marker epithelial cadherin and enhanced cell migration, which effects were inhibited following treatment with PBA or TUDCA. These results indicates that enhanced ER stress induced EMT and subsequently increased cell migration in HLECs in vitro. |
format | Online Article Text |
id | pubmed-6896292 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-68962922019-12-09 Endoplasmic reticulum stress regulates epithelial-mesenchymal transition in human lens epithelial cells Zhou, Sheng Yang, Jing Wang, Mingwei Zheng, Danying Liu, Yizhi Mol Med Rep Articles Epithelial-to-mesenchymal transition (EMT) of human lens epithelial cells (HLECs) serve an important role in cataract formation. The endoplasmic reticulum stress response (ER stress) has been demonstrated to regulate EMT in a number of tissues. The aim of the present study was to demonstrate the role of ER stress on EMT in HLECs. HLECs were treated with tunicamycin (TM) or thapsigargin (TG) to disturb ER homeostasis, and 4-phenylbutyric acid (PBA) or sodium tauroursodeoxycholate (TUDCA) to restore ER homeostasis. Cell morphology was evaluated after 24 h. The long axis and aspect ratio of the cells were analyzed using ImageJ software. The results demonstrated that HLECs adopted an elongated morphology following treatment with TG, and the cellular aspect ratio increased. However, this morphological change was not observed following combination treatment with TG and PBA. Western blot analysis and immunofluorescence staining were used to measure the protein expression levels. A wound-healing assay was performed to evaluate cell migration. Treatment with TM or TG increased the expression of the ER stress markers glucose-regulated protein 78, phosphorylated eukaryotic initiation factor 2α, activating transcription factor (ATF)6, ATF4 and inositol-requiring protein 1α and the EMT markers fibronectin, vimentin, α-smooth muscle actin and neural cadherin. Furthermore, treatment with TM or TG decreased the expression of the epithelial cell marker epithelial cadherin and enhanced cell migration, which effects were inhibited following treatment with PBA or TUDCA. These results indicates that enhanced ER stress induced EMT and subsequently increased cell migration in HLECs in vitro. D.A. Spandidos 2020-01 2019-11-12 /pmc/articles/PMC6896292/ /pubmed/31746423 http://dx.doi.org/10.3892/mmr.2019.10814 Text en Copyright: © Zhou et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zhou, Sheng Yang, Jing Wang, Mingwei Zheng, Danying Liu, Yizhi Endoplasmic reticulum stress regulates epithelial-mesenchymal transition in human lens epithelial cells |
title | Endoplasmic reticulum stress regulates epithelial-mesenchymal transition in human lens epithelial cells |
title_full | Endoplasmic reticulum stress regulates epithelial-mesenchymal transition in human lens epithelial cells |
title_fullStr | Endoplasmic reticulum stress regulates epithelial-mesenchymal transition in human lens epithelial cells |
title_full_unstemmed | Endoplasmic reticulum stress regulates epithelial-mesenchymal transition in human lens epithelial cells |
title_short | Endoplasmic reticulum stress regulates epithelial-mesenchymal transition in human lens epithelial cells |
title_sort | endoplasmic reticulum stress regulates epithelial-mesenchymal transition in human lens epithelial cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896292/ https://www.ncbi.nlm.nih.gov/pubmed/31746423 http://dx.doi.org/10.3892/mmr.2019.10814 |
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