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Multidrug-Resistant and Clinically Relevant Gram-Negative Bacteria Are Present in German Surface Waters

Water is considered to play a role in the dissemination of antibiotic-resistant Gram-negative bacteria including those encoding Extended-spectrum beta-lactamases (ESBL) and carbapenemases. To investigate the role of water for their spread in more detail, we characterized ESBL/Carbapenemase-producing...

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Autores principales: Falgenhauer, Linda, Schwengers, Oliver, Schmiedel, Judith, Baars, Christian, Lambrecht, Oda, Heß, Stefanie, Berendonk, Thomas U., Falgenhauer, Jane, Chakraborty, Trinad, Imirzalioglu, Can
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896662/
https://www.ncbi.nlm.nih.gov/pubmed/31849911
http://dx.doi.org/10.3389/fmicb.2019.02779
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author Falgenhauer, Linda
Schwengers, Oliver
Schmiedel, Judith
Baars, Christian
Lambrecht, Oda
Heß, Stefanie
Berendonk, Thomas U.
Falgenhauer, Jane
Chakraborty, Trinad
Imirzalioglu, Can
author_facet Falgenhauer, Linda
Schwengers, Oliver
Schmiedel, Judith
Baars, Christian
Lambrecht, Oda
Heß, Stefanie
Berendonk, Thomas U.
Falgenhauer, Jane
Chakraborty, Trinad
Imirzalioglu, Can
author_sort Falgenhauer, Linda
collection PubMed
description Water is considered to play a role in the dissemination of antibiotic-resistant Gram-negative bacteria including those encoding Extended-spectrum beta-lactamases (ESBL) and carbapenemases. To investigate the role of water for their spread in more detail, we characterized ESBL/Carbapenemase-producing bacteria from surface water and sediment samples using phenotypic and genotypic approaches. ESBL/Carbapenemase-producing isolates were obtained from water/sediment samples. Species and antibiotic resistance were determined. A subset of these isolates (n = 33) was whole-genome-sequenced and analyzed for the presence of antibiotic resistance genes and virulence determinants. Their relatedness to isolates associated with human infections was investigated using multilocus sequence type and cgMLST-based analysis. Eighty-nine percent of the isolates comprised of clinically relevant species. Fifty-eight percent exhibited a multidrug-resistance phenotype. Two isolates harbored the mobile colistin resistance gene mcr-1. One carbapenemase-producing isolate identified as Enterobacter kobei harbored bla(VIM–)(1). Two Escherichia coli isolates had sequence types (ST) associated with human infections (ST131 and ST1485) and a Klebsiella pneumoniae isolate was classified as hypervirulent. A multidrug-resistant (MDR) Pseudomonas aeruginosa isolate encoding known virulence genes associated with severe lung infections in cystic fibrosis patients was also detected. The presence of MDR and clinically relevant isolates in recreational and surface water underlines the role of aquatic environments as both reservoirs and hot spots for MDR bacteria. Future assessment of water quality should include the examination of the multidrug resistance of clinically relevant bacterial species and thus provide an important link regarding the spread of MDR bacteria in a One Health context.
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spelling pubmed-68966622019-12-17 Multidrug-Resistant and Clinically Relevant Gram-Negative Bacteria Are Present in German Surface Waters Falgenhauer, Linda Schwengers, Oliver Schmiedel, Judith Baars, Christian Lambrecht, Oda Heß, Stefanie Berendonk, Thomas U. Falgenhauer, Jane Chakraborty, Trinad Imirzalioglu, Can Front Microbiol Microbiology Water is considered to play a role in the dissemination of antibiotic-resistant Gram-negative bacteria including those encoding Extended-spectrum beta-lactamases (ESBL) and carbapenemases. To investigate the role of water for their spread in more detail, we characterized ESBL/Carbapenemase-producing bacteria from surface water and sediment samples using phenotypic and genotypic approaches. ESBL/Carbapenemase-producing isolates were obtained from water/sediment samples. Species and antibiotic resistance were determined. A subset of these isolates (n = 33) was whole-genome-sequenced and analyzed for the presence of antibiotic resistance genes and virulence determinants. Their relatedness to isolates associated with human infections was investigated using multilocus sequence type and cgMLST-based analysis. Eighty-nine percent of the isolates comprised of clinically relevant species. Fifty-eight percent exhibited a multidrug-resistance phenotype. Two isolates harbored the mobile colistin resistance gene mcr-1. One carbapenemase-producing isolate identified as Enterobacter kobei harbored bla(VIM–)(1). Two Escherichia coli isolates had sequence types (ST) associated with human infections (ST131 and ST1485) and a Klebsiella pneumoniae isolate was classified as hypervirulent. A multidrug-resistant (MDR) Pseudomonas aeruginosa isolate encoding known virulence genes associated with severe lung infections in cystic fibrosis patients was also detected. The presence of MDR and clinically relevant isolates in recreational and surface water underlines the role of aquatic environments as both reservoirs and hot spots for MDR bacteria. Future assessment of water quality should include the examination of the multidrug resistance of clinically relevant bacterial species and thus provide an important link regarding the spread of MDR bacteria in a One Health context. Frontiers Media S.A. 2019-11-29 /pmc/articles/PMC6896662/ /pubmed/31849911 http://dx.doi.org/10.3389/fmicb.2019.02779 Text en Copyright © 2019 Falgenhauer, Schwengers, Schmiedel, Baars, Lambrecht, Heβ, Berendonk, Falgenhauer, Chakraborty and Imirzalioglu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Falgenhauer, Linda
Schwengers, Oliver
Schmiedel, Judith
Baars, Christian
Lambrecht, Oda
Heß, Stefanie
Berendonk, Thomas U.
Falgenhauer, Jane
Chakraborty, Trinad
Imirzalioglu, Can
Multidrug-Resistant and Clinically Relevant Gram-Negative Bacteria Are Present in German Surface Waters
title Multidrug-Resistant and Clinically Relevant Gram-Negative Bacteria Are Present in German Surface Waters
title_full Multidrug-Resistant and Clinically Relevant Gram-Negative Bacteria Are Present in German Surface Waters
title_fullStr Multidrug-Resistant and Clinically Relevant Gram-Negative Bacteria Are Present in German Surface Waters
title_full_unstemmed Multidrug-Resistant and Clinically Relevant Gram-Negative Bacteria Are Present in German Surface Waters
title_short Multidrug-Resistant and Clinically Relevant Gram-Negative Bacteria Are Present in German Surface Waters
title_sort multidrug-resistant and clinically relevant gram-negative bacteria are present in german surface waters
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896662/
https://www.ncbi.nlm.nih.gov/pubmed/31849911
http://dx.doi.org/10.3389/fmicb.2019.02779
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