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Impaired training-induced angiogenesis process with loss of pericyte-endothelium interactions is associated with an abnormal capillary remodelling in the skeletal muscle of COPD patients

ABSTRACT: Chronic obstructive pulmonary disease (COPD) is associated with exercise intolerance and limits the functional gains in response to exercise training in patients compared to sedentary healthy subjects (SHS). The blunted skeletal muscle angiogenesis previously observed in COPD patients has...

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Autores principales: Blervaque, Léo, Passerieux, Emilie, Pomiès, Pascal, Catteau, Matthias, Héraud, Nelly, Blaquière, Marine, Bughin, François, Ayoub, Bronia, Molinari, Nicolas, Cristol, Jean-Paul, Perez-Martin, Antonia, Mercier, Jacques, Hayot, Maurice, Gouzi, Fares
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896673/
https://www.ncbi.nlm.nih.gov/pubmed/31806021
http://dx.doi.org/10.1186/s12931-019-1240-6
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author Blervaque, Léo
Passerieux, Emilie
Pomiès, Pascal
Catteau, Matthias
Héraud, Nelly
Blaquière, Marine
Bughin, François
Ayoub, Bronia
Molinari, Nicolas
Cristol, Jean-Paul
Perez-Martin, Antonia
Mercier, Jacques
Hayot, Maurice
Gouzi, Fares
author_facet Blervaque, Léo
Passerieux, Emilie
Pomiès, Pascal
Catteau, Matthias
Héraud, Nelly
Blaquière, Marine
Bughin, François
Ayoub, Bronia
Molinari, Nicolas
Cristol, Jean-Paul
Perez-Martin, Antonia
Mercier, Jacques
Hayot, Maurice
Gouzi, Fares
author_sort Blervaque, Léo
collection PubMed
description ABSTRACT: Chronic obstructive pulmonary disease (COPD) is associated with exercise intolerance and limits the functional gains in response to exercise training in patients compared to sedentary healthy subjects (SHS). The blunted skeletal muscle angiogenesis previously observed in COPD patients has been linked to these limited functional improvements, but its underlying mechanisms, as well as the potential role of oxidative stress, remain poorly understood. Therefore, we compared ultrastructural indexes of angiogenic process and capillary remodelling by transmission electron microscopy in 9 COPD patients and 7 SHS after 6 weeks of individualized moderate-intensity endurance training. We also assessed oxidative stress by plasma-free and esterified isoprostane (F(2)-IsoP) levels in both groups. We observed a capillary basement membrane thickening in COPD patients only (p = 0.008) and abnormal variations of endothelial nucleus density in response to exercise training in these patients when compared to SHS (p = 0.042). COPD patients had significantly fewer occurrences of pericyte/endothelium interdigitations, a morphologic marker of capillary maturation, than SHS (p = 0.014), and significantly higher levels of F(2)-IsoP (p = 0.048). Last, the changes in pericyte/endothelium interdigitations and F(2)-IsoP levels in response to exercise training were negatively correlated (r = − 0.62, p = 0.025). This study is the first to show abnormal capillary remodelling and to reveal impairments during the whole process of angiogenesis (capillary creation and maturation) in COPD patients. TRIAL REGISTRATION: NCT01183039 & NCT01183052, both registered 7 August 2010 (retrospectively registered).
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spelling pubmed-68966732019-12-11 Impaired training-induced angiogenesis process with loss of pericyte-endothelium interactions is associated with an abnormal capillary remodelling in the skeletal muscle of COPD patients Blervaque, Léo Passerieux, Emilie Pomiès, Pascal Catteau, Matthias Héraud, Nelly Blaquière, Marine Bughin, François Ayoub, Bronia Molinari, Nicolas Cristol, Jean-Paul Perez-Martin, Antonia Mercier, Jacques Hayot, Maurice Gouzi, Fares Respir Res Research ABSTRACT: Chronic obstructive pulmonary disease (COPD) is associated with exercise intolerance and limits the functional gains in response to exercise training in patients compared to sedentary healthy subjects (SHS). The blunted skeletal muscle angiogenesis previously observed in COPD patients has been linked to these limited functional improvements, but its underlying mechanisms, as well as the potential role of oxidative stress, remain poorly understood. Therefore, we compared ultrastructural indexes of angiogenic process and capillary remodelling by transmission electron microscopy in 9 COPD patients and 7 SHS after 6 weeks of individualized moderate-intensity endurance training. We also assessed oxidative stress by plasma-free and esterified isoprostane (F(2)-IsoP) levels in both groups. We observed a capillary basement membrane thickening in COPD patients only (p = 0.008) and abnormal variations of endothelial nucleus density in response to exercise training in these patients when compared to SHS (p = 0.042). COPD patients had significantly fewer occurrences of pericyte/endothelium interdigitations, a morphologic marker of capillary maturation, than SHS (p = 0.014), and significantly higher levels of F(2)-IsoP (p = 0.048). Last, the changes in pericyte/endothelium interdigitations and F(2)-IsoP levels in response to exercise training were negatively correlated (r = − 0.62, p = 0.025). This study is the first to show abnormal capillary remodelling and to reveal impairments during the whole process of angiogenesis (capillary creation and maturation) in COPD patients. TRIAL REGISTRATION: NCT01183039 & NCT01183052, both registered 7 August 2010 (retrospectively registered). BioMed Central 2019-12-05 2019 /pmc/articles/PMC6896673/ /pubmed/31806021 http://dx.doi.org/10.1186/s12931-019-1240-6 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Blervaque, Léo
Passerieux, Emilie
Pomiès, Pascal
Catteau, Matthias
Héraud, Nelly
Blaquière, Marine
Bughin, François
Ayoub, Bronia
Molinari, Nicolas
Cristol, Jean-Paul
Perez-Martin, Antonia
Mercier, Jacques
Hayot, Maurice
Gouzi, Fares
Impaired training-induced angiogenesis process with loss of pericyte-endothelium interactions is associated with an abnormal capillary remodelling in the skeletal muscle of COPD patients
title Impaired training-induced angiogenesis process with loss of pericyte-endothelium interactions is associated with an abnormal capillary remodelling in the skeletal muscle of COPD patients
title_full Impaired training-induced angiogenesis process with loss of pericyte-endothelium interactions is associated with an abnormal capillary remodelling in the skeletal muscle of COPD patients
title_fullStr Impaired training-induced angiogenesis process with loss of pericyte-endothelium interactions is associated with an abnormal capillary remodelling in the skeletal muscle of COPD patients
title_full_unstemmed Impaired training-induced angiogenesis process with loss of pericyte-endothelium interactions is associated with an abnormal capillary remodelling in the skeletal muscle of COPD patients
title_short Impaired training-induced angiogenesis process with loss of pericyte-endothelium interactions is associated with an abnormal capillary remodelling in the skeletal muscle of COPD patients
title_sort impaired training-induced angiogenesis process with loss of pericyte-endothelium interactions is associated with an abnormal capillary remodelling in the skeletal muscle of copd patients
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896673/
https://www.ncbi.nlm.nih.gov/pubmed/31806021
http://dx.doi.org/10.1186/s12931-019-1240-6
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