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A reference library for Canadian invertebrates with 1.5 million barcodes, voucher specimens, and DNA samples

The reliable taxonomic identification of organisms through DNA sequence data requires a well parameterized library of curated reference sequences. However, it is estimated that just 15% of described animal species are represented in public sequence repositories. To begin to address this deficiency,...

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Autores principales: deWaard, Jeremy R., Ratnasingham, Sujeevan, Zakharov, Evgeny V., Borisenko, Alex V., Steinke, Dirk, Telfer, Angela C., Perez, Kate H. J., Sones, Jayme E., Young, Monica R., Levesque-Beaudin, Valerie, Sobel, Crystal N., Abrahamyan, Arusyak, Bessonov, Kyrylo, Blagoev, Gergin, deWaard, Stephanie L., Ho, Chris, Ivanova, Natalia V., Layton, Kara K. S., Lu, Liuqiong, Manjunath, Ramya, McKeown, Jaclyn T. A., Milton, Megan A., Miskie, Renee, Monkhouse, Norm, Naik, Suresh, Nikolova, Nadya, Pentinsaari, Mikko, Prosser, Sean W. J., Radulovici, Adriana E., Steinke, Claudia, Warne, Connor P., Hebert, Paul D. N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6897906/
https://www.ncbi.nlm.nih.gov/pubmed/31811161
http://dx.doi.org/10.1038/s41597-019-0320-2
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author deWaard, Jeremy R.
Ratnasingham, Sujeevan
Zakharov, Evgeny V.
Borisenko, Alex V.
Steinke, Dirk
Telfer, Angela C.
Perez, Kate H. J.
Sones, Jayme E.
Young, Monica R.
Levesque-Beaudin, Valerie
Sobel, Crystal N.
Abrahamyan, Arusyak
Bessonov, Kyrylo
Blagoev, Gergin
deWaard, Stephanie L.
Ho, Chris
Ivanova, Natalia V.
Layton, Kara K. S.
Lu, Liuqiong
Manjunath, Ramya
McKeown, Jaclyn T. A.
Milton, Megan A.
Miskie, Renee
Monkhouse, Norm
Naik, Suresh
Nikolova, Nadya
Pentinsaari, Mikko
Prosser, Sean W. J.
Radulovici, Adriana E.
Steinke, Claudia
Warne, Connor P.
Hebert, Paul D. N.
author_facet deWaard, Jeremy R.
Ratnasingham, Sujeevan
Zakharov, Evgeny V.
Borisenko, Alex V.
Steinke, Dirk
Telfer, Angela C.
Perez, Kate H. J.
Sones, Jayme E.
Young, Monica R.
Levesque-Beaudin, Valerie
Sobel, Crystal N.
Abrahamyan, Arusyak
Bessonov, Kyrylo
Blagoev, Gergin
deWaard, Stephanie L.
Ho, Chris
Ivanova, Natalia V.
Layton, Kara K. S.
Lu, Liuqiong
Manjunath, Ramya
McKeown, Jaclyn T. A.
Milton, Megan A.
Miskie, Renee
Monkhouse, Norm
Naik, Suresh
Nikolova, Nadya
Pentinsaari, Mikko
Prosser, Sean W. J.
Radulovici, Adriana E.
Steinke, Claudia
Warne, Connor P.
Hebert, Paul D. N.
author_sort deWaard, Jeremy R.
collection PubMed
description The reliable taxonomic identification of organisms through DNA sequence data requires a well parameterized library of curated reference sequences. However, it is estimated that just 15% of described animal species are represented in public sequence repositories. To begin to address this deficiency, we provide DNA barcodes for 1,500,003 animal specimens collected from 23 terrestrial and aquatic ecozones at sites across Canada, a nation that comprises 7% of the planet’s land surface. In total, 14 phyla, 43 classes, 163 orders, 1123 families, 6186 genera, and 64,264 Barcode Index Numbers (BINs; a proxy for species) are represented. Species-level taxonomy was available for 38% of the specimens, but higher proportions were assigned to a genus (69.5%) and a family (99.9%). Voucher specimens and DNA extracts are archived at the Centre for Biodiversity Genomics where they are available for further research. The corresponding sequence and taxonomic data can be accessed through the Barcode of Life Data System, GenBank, the Global Biodiversity Information Facility, and the Global Genome Biodiversity Network Data Portal.
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spelling pubmed-68979062019-12-13 A reference library for Canadian invertebrates with 1.5 million barcodes, voucher specimens, and DNA samples deWaard, Jeremy R. Ratnasingham, Sujeevan Zakharov, Evgeny V. Borisenko, Alex V. Steinke, Dirk Telfer, Angela C. Perez, Kate H. J. Sones, Jayme E. Young, Monica R. Levesque-Beaudin, Valerie Sobel, Crystal N. Abrahamyan, Arusyak Bessonov, Kyrylo Blagoev, Gergin deWaard, Stephanie L. Ho, Chris Ivanova, Natalia V. Layton, Kara K. S. Lu, Liuqiong Manjunath, Ramya McKeown, Jaclyn T. A. Milton, Megan A. Miskie, Renee Monkhouse, Norm Naik, Suresh Nikolova, Nadya Pentinsaari, Mikko Prosser, Sean W. J. Radulovici, Adriana E. Steinke, Claudia Warne, Connor P. Hebert, Paul D. N. Sci Data Data Descriptor The reliable taxonomic identification of organisms through DNA sequence data requires a well parameterized library of curated reference sequences. However, it is estimated that just 15% of described animal species are represented in public sequence repositories. To begin to address this deficiency, we provide DNA barcodes for 1,500,003 animal specimens collected from 23 terrestrial and aquatic ecozones at sites across Canada, a nation that comprises 7% of the planet’s land surface. In total, 14 phyla, 43 classes, 163 orders, 1123 families, 6186 genera, and 64,264 Barcode Index Numbers (BINs; a proxy for species) are represented. Species-level taxonomy was available for 38% of the specimens, but higher proportions were assigned to a genus (69.5%) and a family (99.9%). Voucher specimens and DNA extracts are archived at the Centre for Biodiversity Genomics where they are available for further research. The corresponding sequence and taxonomic data can be accessed through the Barcode of Life Data System, GenBank, the Global Biodiversity Information Facility, and the Global Genome Biodiversity Network Data Portal. Nature Publishing Group UK 2019-12-06 /pmc/articles/PMC6897906/ /pubmed/31811161 http://dx.doi.org/10.1038/s41597-019-0320-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the metadata files associated with this article.
spellingShingle Data Descriptor
deWaard, Jeremy R.
Ratnasingham, Sujeevan
Zakharov, Evgeny V.
Borisenko, Alex V.
Steinke, Dirk
Telfer, Angela C.
Perez, Kate H. J.
Sones, Jayme E.
Young, Monica R.
Levesque-Beaudin, Valerie
Sobel, Crystal N.
Abrahamyan, Arusyak
Bessonov, Kyrylo
Blagoev, Gergin
deWaard, Stephanie L.
Ho, Chris
Ivanova, Natalia V.
Layton, Kara K. S.
Lu, Liuqiong
Manjunath, Ramya
McKeown, Jaclyn T. A.
Milton, Megan A.
Miskie, Renee
Monkhouse, Norm
Naik, Suresh
Nikolova, Nadya
Pentinsaari, Mikko
Prosser, Sean W. J.
Radulovici, Adriana E.
Steinke, Claudia
Warne, Connor P.
Hebert, Paul D. N.
A reference library for Canadian invertebrates with 1.5 million barcodes, voucher specimens, and DNA samples
title A reference library for Canadian invertebrates with 1.5 million barcodes, voucher specimens, and DNA samples
title_full A reference library for Canadian invertebrates with 1.5 million barcodes, voucher specimens, and DNA samples
title_fullStr A reference library for Canadian invertebrates with 1.5 million barcodes, voucher specimens, and DNA samples
title_full_unstemmed A reference library for Canadian invertebrates with 1.5 million barcodes, voucher specimens, and DNA samples
title_short A reference library for Canadian invertebrates with 1.5 million barcodes, voucher specimens, and DNA samples
title_sort reference library for canadian invertebrates with 1.5 million barcodes, voucher specimens, and dna samples
topic Data Descriptor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6897906/
https://www.ncbi.nlm.nih.gov/pubmed/31811161
http://dx.doi.org/10.1038/s41597-019-0320-2
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