Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation

Life on the molecular scale is based on a complex interplay of biomolecules under which the ability of binding is crucial. Fluorescence based two-color coincidence detection (TCCD) is commonly used to characterize molecular binding, but suffers from an underestimation of coincident events. Here, we...

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Autores principales: Höfig, Henning, Yukhnovets, Olessya, Remes, Cristina, Kempf, Noemie, Katranidis, Alexandros, Kempe, Daryan, Fitter, Jörg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6897966/
https://www.ncbi.nlm.nih.gov/pubmed/31840104
http://dx.doi.org/10.1038/s42003-019-0709-7
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author Höfig, Henning
Yukhnovets, Olessya
Remes, Cristina
Kempf, Noemie
Katranidis, Alexandros
Kempe, Daryan
Fitter, Jörg
author_facet Höfig, Henning
Yukhnovets, Olessya
Remes, Cristina
Kempf, Noemie
Katranidis, Alexandros
Kempe, Daryan
Fitter, Jörg
author_sort Höfig, Henning
collection PubMed
description Life on the molecular scale is based on a complex interplay of biomolecules under which the ability of binding is crucial. Fluorescence based two-color coincidence detection (TCCD) is commonly used to characterize molecular binding, but suffers from an underestimation of coincident events. Here, we introduce a brightness-gated TCCD which overcomes this limitation and benchmark our approach with two custom-made calibration samples. Applied to a cell-free protein synthesis assay, brightness-gated TCCD unraveled a previously disregarded mode of translation initiation in bacteria.
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spelling pubmed-68979662019-12-13 Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation Höfig, Henning Yukhnovets, Olessya Remes, Cristina Kempf, Noemie Katranidis, Alexandros Kempe, Daryan Fitter, Jörg Commun Biol Article Life on the molecular scale is based on a complex interplay of biomolecules under which the ability of binding is crucial. Fluorescence based two-color coincidence detection (TCCD) is commonly used to characterize molecular binding, but suffers from an underestimation of coincident events. Here, we introduce a brightness-gated TCCD which overcomes this limitation and benchmark our approach with two custom-made calibration samples. Applied to a cell-free protein synthesis assay, brightness-gated TCCD unraveled a previously disregarded mode of translation initiation in bacteria. Nature Publishing Group UK 2019-12-06 /pmc/articles/PMC6897966/ /pubmed/31840104 http://dx.doi.org/10.1038/s42003-019-0709-7 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Höfig, Henning
Yukhnovets, Olessya
Remes, Cristina
Kempf, Noemie
Katranidis, Alexandros
Kempe, Daryan
Fitter, Jörg
Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation
title Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation
title_full Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation
title_fullStr Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation
title_full_unstemmed Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation
title_short Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation
title_sort brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6897966/
https://www.ncbi.nlm.nih.gov/pubmed/31840104
http://dx.doi.org/10.1038/s42003-019-0709-7
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