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Essential roles of autophagy in metabolic regulation in endosperm development during rice seed maturation

Autophagy plays crucial roles in the recycling of metabolites, and is involved in many developmental processes. Rice mutants defective in autophagy are male sterile due to immature pollens, indicating its critical role in pollen development. However, physiological roles of autophagy during seed matu...

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Autores principales: Sera, Yuri, Hanamata, Shigeru, Sakamoto, Shingo, Ono, Seijiro, Kaneko, Kentaro, Mitsui, Yuudai, Koyano, Tomoko, Fujita, Naoko, Sasou, Ai, Masumura, Takehiro, Saji, Hikaru, Nonomura, Ken-Ichi, Mitsuda, Nobutaka, Mitsui, Toshiaki, Kurusu, Takamitsu, Kuchitsu, Kazuyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6898296/
https://www.ncbi.nlm.nih.gov/pubmed/31811157
http://dx.doi.org/10.1038/s41598-019-54361-1
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author Sera, Yuri
Hanamata, Shigeru
Sakamoto, Shingo
Ono, Seijiro
Kaneko, Kentaro
Mitsui, Yuudai
Koyano, Tomoko
Fujita, Naoko
Sasou, Ai
Masumura, Takehiro
Saji, Hikaru
Nonomura, Ken-Ichi
Mitsuda, Nobutaka
Mitsui, Toshiaki
Kurusu, Takamitsu
Kuchitsu, Kazuyuki
author_facet Sera, Yuri
Hanamata, Shigeru
Sakamoto, Shingo
Ono, Seijiro
Kaneko, Kentaro
Mitsui, Yuudai
Koyano, Tomoko
Fujita, Naoko
Sasou, Ai
Masumura, Takehiro
Saji, Hikaru
Nonomura, Ken-Ichi
Mitsuda, Nobutaka
Mitsui, Toshiaki
Kurusu, Takamitsu
Kuchitsu, Kazuyuki
author_sort Sera, Yuri
collection PubMed
description Autophagy plays crucial roles in the recycling of metabolites, and is involved in many developmental processes. Rice mutants defective in autophagy are male sterile due to immature pollens, indicating its critical role in pollen development. However, physiological roles of autophagy during seed maturation had remained unknown. We here found that seeds of the rice autophagy-deficient mutant Osatg7-1, that produces seeds at a very low frequency in paddy fields, are smaller and show chalky appearance and lower starch content in the endosperm at the mature stage under normal growth condition. We comprehensively analyzed the effects of disruption of autophagy on biochemical properties, proteome and seed quality, and found an abnormal activation of starch degradation pathways including accumulation of α-amylases in the endosperm during seed maturation in Osatg7-1. These results indicate critical involvement of autophagy in metabolic regulation in the endosperm of rice, and provide insights into novel autophagy-mediated regulation of starch metabolism during seed maturation.
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spelling pubmed-68982962019-12-12 Essential roles of autophagy in metabolic regulation in endosperm development during rice seed maturation Sera, Yuri Hanamata, Shigeru Sakamoto, Shingo Ono, Seijiro Kaneko, Kentaro Mitsui, Yuudai Koyano, Tomoko Fujita, Naoko Sasou, Ai Masumura, Takehiro Saji, Hikaru Nonomura, Ken-Ichi Mitsuda, Nobutaka Mitsui, Toshiaki Kurusu, Takamitsu Kuchitsu, Kazuyuki Sci Rep Article Autophagy plays crucial roles in the recycling of metabolites, and is involved in many developmental processes. Rice mutants defective in autophagy are male sterile due to immature pollens, indicating its critical role in pollen development. However, physiological roles of autophagy during seed maturation had remained unknown. We here found that seeds of the rice autophagy-deficient mutant Osatg7-1, that produces seeds at a very low frequency in paddy fields, are smaller and show chalky appearance and lower starch content in the endosperm at the mature stage under normal growth condition. We comprehensively analyzed the effects of disruption of autophagy on biochemical properties, proteome and seed quality, and found an abnormal activation of starch degradation pathways including accumulation of α-amylases in the endosperm during seed maturation in Osatg7-1. These results indicate critical involvement of autophagy in metabolic regulation in the endosperm of rice, and provide insights into novel autophagy-mediated regulation of starch metabolism during seed maturation. Nature Publishing Group UK 2019-12-06 /pmc/articles/PMC6898296/ /pubmed/31811157 http://dx.doi.org/10.1038/s41598-019-54361-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sera, Yuri
Hanamata, Shigeru
Sakamoto, Shingo
Ono, Seijiro
Kaneko, Kentaro
Mitsui, Yuudai
Koyano, Tomoko
Fujita, Naoko
Sasou, Ai
Masumura, Takehiro
Saji, Hikaru
Nonomura, Ken-Ichi
Mitsuda, Nobutaka
Mitsui, Toshiaki
Kurusu, Takamitsu
Kuchitsu, Kazuyuki
Essential roles of autophagy in metabolic regulation in endosperm development during rice seed maturation
title Essential roles of autophagy in metabolic regulation in endosperm development during rice seed maturation
title_full Essential roles of autophagy in metabolic regulation in endosperm development during rice seed maturation
title_fullStr Essential roles of autophagy in metabolic regulation in endosperm development during rice seed maturation
title_full_unstemmed Essential roles of autophagy in metabolic regulation in endosperm development during rice seed maturation
title_short Essential roles of autophagy in metabolic regulation in endosperm development during rice seed maturation
title_sort essential roles of autophagy in metabolic regulation in endosperm development during rice seed maturation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6898296/
https://www.ncbi.nlm.nih.gov/pubmed/31811157
http://dx.doi.org/10.1038/s41598-019-54361-1
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