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High-resolution label-free 3D mapping of extracellular pH of single living cells

Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H(+) in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level...

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Autores principales: Zhang, Yanjun, Takahashi, Yasufumi, Hong, Sung Pil, Liu, Fengjie, Bednarska, Joanna, Goff, Philip S., Novak, Pavel, Shevchuk, Andrew, Gopal, Sahana, Barozzi, Iros, Magnani, Luca, Sakai, Hideki, Suguru, Yoshimoto, Fujii, Takuto, Erofeev, Alexander, Gorelkin, Peter, Majouga, Alexander, Weiss, Dominik J., Edwards, Christopher, Ivanov, Aleksandar P., Klenerman, David, Sviderskaya, Elena V., Edel, Joshua B., Korchev, Yuri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6898398/
https://www.ncbi.nlm.nih.gov/pubmed/31811139
http://dx.doi.org/10.1038/s41467-019-13535-1
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author Zhang, Yanjun
Takahashi, Yasufumi
Hong, Sung Pil
Liu, Fengjie
Bednarska, Joanna
Goff, Philip S.
Novak, Pavel
Shevchuk, Andrew
Gopal, Sahana
Barozzi, Iros
Magnani, Luca
Sakai, Hideki
Suguru, Yoshimoto
Fujii, Takuto
Erofeev, Alexander
Gorelkin, Peter
Majouga, Alexander
Weiss, Dominik J.
Edwards, Christopher
Ivanov, Aleksandar P.
Klenerman, David
Sviderskaya, Elena V.
Edel, Joshua B.
Korchev, Yuri
author_facet Zhang, Yanjun
Takahashi, Yasufumi
Hong, Sung Pil
Liu, Fengjie
Bednarska, Joanna
Goff, Philip S.
Novak, Pavel
Shevchuk, Andrew
Gopal, Sahana
Barozzi, Iros
Magnani, Luca
Sakai, Hideki
Suguru, Yoshimoto
Fujii, Takuto
Erofeev, Alexander
Gorelkin, Peter
Majouga, Alexander
Weiss, Dominik J.
Edwards, Christopher
Ivanov, Aleksandar P.
Klenerman, David
Sviderskaya, Elena V.
Edel, Joshua B.
Korchev, Yuri
author_sort Zhang, Yanjun
collection PubMed
description Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H(+) in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells.
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spelling pubmed-68983982019-12-09 High-resolution label-free 3D mapping of extracellular pH of single living cells Zhang, Yanjun Takahashi, Yasufumi Hong, Sung Pil Liu, Fengjie Bednarska, Joanna Goff, Philip S. Novak, Pavel Shevchuk, Andrew Gopal, Sahana Barozzi, Iros Magnani, Luca Sakai, Hideki Suguru, Yoshimoto Fujii, Takuto Erofeev, Alexander Gorelkin, Peter Majouga, Alexander Weiss, Dominik J. Edwards, Christopher Ivanov, Aleksandar P. Klenerman, David Sviderskaya, Elena V. Edel, Joshua B. Korchev, Yuri Nat Commun Article Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H(+) in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells. Nature Publishing Group UK 2019-12-06 /pmc/articles/PMC6898398/ /pubmed/31811139 http://dx.doi.org/10.1038/s41467-019-13535-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhang, Yanjun
Takahashi, Yasufumi
Hong, Sung Pil
Liu, Fengjie
Bednarska, Joanna
Goff, Philip S.
Novak, Pavel
Shevchuk, Andrew
Gopal, Sahana
Barozzi, Iros
Magnani, Luca
Sakai, Hideki
Suguru, Yoshimoto
Fujii, Takuto
Erofeev, Alexander
Gorelkin, Peter
Majouga, Alexander
Weiss, Dominik J.
Edwards, Christopher
Ivanov, Aleksandar P.
Klenerman, David
Sviderskaya, Elena V.
Edel, Joshua B.
Korchev, Yuri
High-resolution label-free 3D mapping of extracellular pH of single living cells
title High-resolution label-free 3D mapping of extracellular pH of single living cells
title_full High-resolution label-free 3D mapping of extracellular pH of single living cells
title_fullStr High-resolution label-free 3D mapping of extracellular pH of single living cells
title_full_unstemmed High-resolution label-free 3D mapping of extracellular pH of single living cells
title_short High-resolution label-free 3D mapping of extracellular pH of single living cells
title_sort high-resolution label-free 3d mapping of extracellular ph of single living cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6898398/
https://www.ncbi.nlm.nih.gov/pubmed/31811139
http://dx.doi.org/10.1038/s41467-019-13535-1
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