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High-resolution label-free 3D mapping of extracellular pH of single living cells
Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H(+) in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6898398/ https://www.ncbi.nlm.nih.gov/pubmed/31811139 http://dx.doi.org/10.1038/s41467-019-13535-1 |
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author | Zhang, Yanjun Takahashi, Yasufumi Hong, Sung Pil Liu, Fengjie Bednarska, Joanna Goff, Philip S. Novak, Pavel Shevchuk, Andrew Gopal, Sahana Barozzi, Iros Magnani, Luca Sakai, Hideki Suguru, Yoshimoto Fujii, Takuto Erofeev, Alexander Gorelkin, Peter Majouga, Alexander Weiss, Dominik J. Edwards, Christopher Ivanov, Aleksandar P. Klenerman, David Sviderskaya, Elena V. Edel, Joshua B. Korchev, Yuri |
author_facet | Zhang, Yanjun Takahashi, Yasufumi Hong, Sung Pil Liu, Fengjie Bednarska, Joanna Goff, Philip S. Novak, Pavel Shevchuk, Andrew Gopal, Sahana Barozzi, Iros Magnani, Luca Sakai, Hideki Suguru, Yoshimoto Fujii, Takuto Erofeev, Alexander Gorelkin, Peter Majouga, Alexander Weiss, Dominik J. Edwards, Christopher Ivanov, Aleksandar P. Klenerman, David Sviderskaya, Elena V. Edel, Joshua B. Korchev, Yuri |
author_sort | Zhang, Yanjun |
collection | PubMed |
description | Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H(+) in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells. |
format | Online Article Text |
id | pubmed-6898398 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-68983982019-12-09 High-resolution label-free 3D mapping of extracellular pH of single living cells Zhang, Yanjun Takahashi, Yasufumi Hong, Sung Pil Liu, Fengjie Bednarska, Joanna Goff, Philip S. Novak, Pavel Shevchuk, Andrew Gopal, Sahana Barozzi, Iros Magnani, Luca Sakai, Hideki Suguru, Yoshimoto Fujii, Takuto Erofeev, Alexander Gorelkin, Peter Majouga, Alexander Weiss, Dominik J. Edwards, Christopher Ivanov, Aleksandar P. Klenerman, David Sviderskaya, Elena V. Edel, Joshua B. Korchev, Yuri Nat Commun Article Dynamic mapping of extracellular pH (pHe) at the single-cell level is critical for understanding the role of H(+) in cellular and subcellular processes, with particular importance in cancer. While several pHe sensing techniques have been developed, accessing this information at the single-cell level requires improvement in sensitivity, spatial and temporal resolution. We report on a zwitterionic label-free pH nanoprobe that addresses these long-standing challenges. The probe has a sensitivity > 0.01 units, 2 ms response time, and 50 nm spatial resolution. The platform was integrated into a double-barrel nanoprobe combining pH sensing with feedback-controlled distance dependance via Scanning Ion Conductance Microscopy. This allows for the simultaneous 3D topographical imaging and pHe monitoring of living cancer cells. These classes of nanoprobes were used for real-time high spatiotemporal resolution pHe mapping at the subcellular level and revealed tumour heterogeneity of the peri-cellular environments of melanoma and breast cancer cells. Nature Publishing Group UK 2019-12-06 /pmc/articles/PMC6898398/ /pubmed/31811139 http://dx.doi.org/10.1038/s41467-019-13535-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Zhang, Yanjun Takahashi, Yasufumi Hong, Sung Pil Liu, Fengjie Bednarska, Joanna Goff, Philip S. Novak, Pavel Shevchuk, Andrew Gopal, Sahana Barozzi, Iros Magnani, Luca Sakai, Hideki Suguru, Yoshimoto Fujii, Takuto Erofeev, Alexander Gorelkin, Peter Majouga, Alexander Weiss, Dominik J. Edwards, Christopher Ivanov, Aleksandar P. Klenerman, David Sviderskaya, Elena V. Edel, Joshua B. Korchev, Yuri High-resolution label-free 3D mapping of extracellular pH of single living cells |
title | High-resolution label-free 3D mapping of extracellular pH of single living cells |
title_full | High-resolution label-free 3D mapping of extracellular pH of single living cells |
title_fullStr | High-resolution label-free 3D mapping of extracellular pH of single living cells |
title_full_unstemmed | High-resolution label-free 3D mapping of extracellular pH of single living cells |
title_short | High-resolution label-free 3D mapping of extracellular pH of single living cells |
title_sort | high-resolution label-free 3d mapping of extracellular ph of single living cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6898398/ https://www.ncbi.nlm.nih.gov/pubmed/31811139 http://dx.doi.org/10.1038/s41467-019-13535-1 |
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