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Influence of pure fluorides and stannous ions on the initial bacterial colonization in situ

The present clinical-experimental study aims to examine the effect of pure experimental fluoride solutions and stannous chloride on the initial oral bioadhesion under in situ conditions. After 1 min of pellicle formation on bovine enamel slabs, 12 subjects rinsed with 8 ml of the fluoride test solut...

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Autores principales: Jasmin, Kirsch, Matthias, Hannig, Pia, Winkel, Sabine, Basche, Birgit, Leis, Norbert, Pütz, Anna, Kensche, Christian, Hannig
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6898421/
https://www.ncbi.nlm.nih.gov/pubmed/31811248
http://dx.doi.org/10.1038/s41598-019-55083-0
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author Jasmin, Kirsch
Matthias, Hannig
Pia, Winkel
Sabine, Basche
Birgit, Leis
Norbert, Pütz
Anna, Kensche
Christian, Hannig
author_facet Jasmin, Kirsch
Matthias, Hannig
Pia, Winkel
Sabine, Basche
Birgit, Leis
Norbert, Pütz
Anna, Kensche
Christian, Hannig
author_sort Jasmin, Kirsch
collection PubMed
description The present clinical-experimental study aims to examine the effect of pure experimental fluoride solutions and stannous chloride on the initial oral bioadhesion under in situ conditions. After 1 min of pellicle formation on bovine enamel slabs, 12 subjects rinsed with 8 ml of the fluoride test solutions (NaF, Na(2)PO(3)F, AmF, SnF(2),) with 500 ppm fluoride concentration each for 1 min. Additionally, rinsing without a solution (control) and rinsing with 1563 ppm SnCl(2) solution took place for 1 min. Afterwards, fluorescence microscopy took place to visualize bacterial adhesion and glucan formation (8 h oral exposition) with DAPI and ConA and the BacLight method. TEM was performed to visualize the pellicle ultrastructure together with EDX to detect stannous ions. The rinsing solutions with pure SnF(2) and SnCl(2) reduced significantly the initial bacterial colonization (DAPI). While, NaF and Na(2)PO(3)F showed no significant effect compared to the control. There was no significant difference between AmF, SnF(2) and SnCl(2). All tested experimental solutions showed no reducing effect on the glucan formation. Considerable alterations of the pellicle ultrastructure resulted from rinsing with the Sn-containing solutions. SnF(2) appears to be the most effective type of fluoride to reduce initial bacterial colonization in situ. The observed effects primarily have to be attributed to the stannous ions’ content.
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spelling pubmed-68984212019-12-12 Influence of pure fluorides and stannous ions on the initial bacterial colonization in situ Jasmin, Kirsch Matthias, Hannig Pia, Winkel Sabine, Basche Birgit, Leis Norbert, Pütz Anna, Kensche Christian, Hannig Sci Rep Article The present clinical-experimental study aims to examine the effect of pure experimental fluoride solutions and stannous chloride on the initial oral bioadhesion under in situ conditions. After 1 min of pellicle formation on bovine enamel slabs, 12 subjects rinsed with 8 ml of the fluoride test solutions (NaF, Na(2)PO(3)F, AmF, SnF(2),) with 500 ppm fluoride concentration each for 1 min. Additionally, rinsing without a solution (control) and rinsing with 1563 ppm SnCl(2) solution took place for 1 min. Afterwards, fluorescence microscopy took place to visualize bacterial adhesion and glucan formation (8 h oral exposition) with DAPI and ConA and the BacLight method. TEM was performed to visualize the pellicle ultrastructure together with EDX to detect stannous ions. The rinsing solutions with pure SnF(2) and SnCl(2) reduced significantly the initial bacterial colonization (DAPI). While, NaF and Na(2)PO(3)F showed no significant effect compared to the control. There was no significant difference between AmF, SnF(2) and SnCl(2). All tested experimental solutions showed no reducing effect on the glucan formation. Considerable alterations of the pellicle ultrastructure resulted from rinsing with the Sn-containing solutions. SnF(2) appears to be the most effective type of fluoride to reduce initial bacterial colonization in situ. The observed effects primarily have to be attributed to the stannous ions’ content. Nature Publishing Group UK 2019-12-06 /pmc/articles/PMC6898421/ /pubmed/31811248 http://dx.doi.org/10.1038/s41598-019-55083-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Jasmin, Kirsch
Matthias, Hannig
Pia, Winkel
Sabine, Basche
Birgit, Leis
Norbert, Pütz
Anna, Kensche
Christian, Hannig
Influence of pure fluorides and stannous ions on the initial bacterial colonization in situ
title Influence of pure fluorides and stannous ions on the initial bacterial colonization in situ
title_full Influence of pure fluorides and stannous ions on the initial bacterial colonization in situ
title_fullStr Influence of pure fluorides and stannous ions on the initial bacterial colonization in situ
title_full_unstemmed Influence of pure fluorides and stannous ions on the initial bacterial colonization in situ
title_short Influence of pure fluorides and stannous ions on the initial bacterial colonization in situ
title_sort influence of pure fluorides and stannous ions on the initial bacterial colonization in situ
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6898421/
https://www.ncbi.nlm.nih.gov/pubmed/31811248
http://dx.doi.org/10.1038/s41598-019-55083-0
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