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Genome-wide DNA methylation profile in the peripheral blood of cocaine and crack dependents
OBJECTIVE: Cocaine use disorders (CUDs) represent a major public health problem in many countries. To better understand the interaction between the environmental modulations and phenotype, the aim of the present study was to investigate the DNA methylation pattern of CUD patients, who had concomitan...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Associação Brasileira de Psiquiatria
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6899365/ https://www.ncbi.nlm.nih.gov/pubmed/31116258 http://dx.doi.org/10.1590/1516-4446-2018-0092 |
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author | Camilo, Caroline Maschietto, Mariana Vieira, Henrique C. Tahira, Ana C. Gouveia, Gisele R. Feio dos Santos, Ana C. Negrão, André B. Ribeiro, Marcelo Laranjeira, Ronaldo Vallada, Homero Brentani, Helena |
author_facet | Camilo, Caroline Maschietto, Mariana Vieira, Henrique C. Tahira, Ana C. Gouveia, Gisele R. Feio dos Santos, Ana C. Negrão, André B. Ribeiro, Marcelo Laranjeira, Ronaldo Vallada, Homero Brentani, Helena |
author_sort | Camilo, Caroline |
collection | PubMed |
description | OBJECTIVE: Cocaine use disorders (CUDs) represent a major public health problem in many countries. To better understand the interaction between the environmental modulations and phenotype, the aim of the present study was to investigate the DNA methylation pattern of CUD patients, who had concomitant cocaine and crack dependence, and healthy controls. METHODS: We studied DNA methylation profiles in the peripheral blood of 23 CUD patients and 24 healthy control subjects using the Illumina Infinium HumanMethylation450 BeadChip arrays. RESULTS: Comparison between CUD patients and controls revealed 186 differentially methylated positions (DMPs; adjusted p-value [adjP] < 10(-5)) related to 152 genes, with a subset of CpGs confirmed by pyrosequencing. DNA methylation patterns discriminated CUD patients and control groups. A gene network approach showed that the EHMT1, EHMT2, MAPK1, MAPK3, MAP2K1, and HDAC5 genes, which are involved in transcription and chromatin regulation cellular signaling pathways, were also associated with cocaine dependence. CONCLUSION: The investigation of DNA methylation patterns may contribute to a better understanding of the biological mechanisms involved in CUD. |
format | Online Article Text |
id | pubmed-6899365 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Associação Brasileira de Psiquiatria |
record_format | MEDLINE/PubMed |
spelling | pubmed-68993652019-12-30 Genome-wide DNA methylation profile in the peripheral blood of cocaine and crack dependents Camilo, Caroline Maschietto, Mariana Vieira, Henrique C. Tahira, Ana C. Gouveia, Gisele R. Feio dos Santos, Ana C. Negrão, André B. Ribeiro, Marcelo Laranjeira, Ronaldo Vallada, Homero Brentani, Helena Braz J Psychiatry Original Article OBJECTIVE: Cocaine use disorders (CUDs) represent a major public health problem in many countries. To better understand the interaction between the environmental modulations and phenotype, the aim of the present study was to investigate the DNA methylation pattern of CUD patients, who had concomitant cocaine and crack dependence, and healthy controls. METHODS: We studied DNA methylation profiles in the peripheral blood of 23 CUD patients and 24 healthy control subjects using the Illumina Infinium HumanMethylation450 BeadChip arrays. RESULTS: Comparison between CUD patients and controls revealed 186 differentially methylated positions (DMPs; adjusted p-value [adjP] < 10(-5)) related to 152 genes, with a subset of CpGs confirmed by pyrosequencing. DNA methylation patterns discriminated CUD patients and control groups. A gene network approach showed that the EHMT1, EHMT2, MAPK1, MAPK3, MAP2K1, and HDAC5 genes, which are involved in transcription and chromatin regulation cellular signaling pathways, were also associated with cocaine dependence. CONCLUSION: The investigation of DNA methylation patterns may contribute to a better understanding of the biological mechanisms involved in CUD. Associação Brasileira de Psiquiatria 2019-05-16 /pmc/articles/PMC6899365/ /pubmed/31116258 http://dx.doi.org/10.1590/1516-4446-2018-0092 Text en http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Camilo, Caroline Maschietto, Mariana Vieira, Henrique C. Tahira, Ana C. Gouveia, Gisele R. Feio dos Santos, Ana C. Negrão, André B. Ribeiro, Marcelo Laranjeira, Ronaldo Vallada, Homero Brentani, Helena Genome-wide DNA methylation profile in the peripheral blood of cocaine and crack dependents |
title | Genome-wide DNA methylation profile in the peripheral blood of cocaine and crack dependents |
title_full | Genome-wide DNA methylation profile in the peripheral blood of cocaine and crack dependents |
title_fullStr | Genome-wide DNA methylation profile in the peripheral blood of cocaine and crack dependents |
title_full_unstemmed | Genome-wide DNA methylation profile in the peripheral blood of cocaine and crack dependents |
title_short | Genome-wide DNA methylation profile in the peripheral blood of cocaine and crack dependents |
title_sort | genome-wide dna methylation profile in the peripheral blood of cocaine and crack dependents |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6899365/ https://www.ncbi.nlm.nih.gov/pubmed/31116258 http://dx.doi.org/10.1590/1516-4446-2018-0092 |
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