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Intermittent hypoxia, brain glyoxalase-1 and glutathione reductase-1, and anxiety-like behavior in mice

OBJECTIVE: Sleep apnea has been associated with anxiety, but the mechanisms of the sleep apnea-anxiety relationship are unresolved. Sleep apnea causes oxidative stress, which might enhance anxiety-like behavior in rodents. To clarify the apnea-anxiety connection, we tested the effect of intermittent...

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Autores principales: Carissimi, Alicia, Martinez, Denis, Kim, Lenise J., Fiori, Cintia Z., Vieira, Luciana R., Rosa, Darlan P., Pires, Gabriel N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Associação Brasileira de Psiquiatria 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6899376/
https://www.ncbi.nlm.nih.gov/pubmed/30110090
http://dx.doi.org/10.1590/1516-4446-2017-2310
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author Carissimi, Alicia
Martinez, Denis
Kim, Lenise J.
Fiori, Cintia Z.
Vieira, Luciana R.
Rosa, Darlan P.
Pires, Gabriel N.
author_facet Carissimi, Alicia
Martinez, Denis
Kim, Lenise J.
Fiori, Cintia Z.
Vieira, Luciana R.
Rosa, Darlan P.
Pires, Gabriel N.
author_sort Carissimi, Alicia
collection PubMed
description OBJECTIVE: Sleep apnea has been associated with anxiety, but the mechanisms of the sleep apnea-anxiety relationship are unresolved. Sleep apnea causes oxidative stress, which might enhance anxiety-like behavior in rodents. To clarify the apnea-anxiety connection, we tested the effect of intermittent hypoxia, a model of sleep apnea, on the anxiety behavior of mice. METHODS: The rodents were exposed daily to 480 one-minute cycles of intermittent hypoxia to a nadir of 7±1% inspiratory oxygen fraction or to a sham procedure with room air. After 7 days, the mice from both groups were placed in an elevated plus maze and were video recorded for 10 min to allow analysis of latency, frequency, and duration in open and closed arms. Glyoxalase-1 (Glo1) and glutathione reductase-1 (GR1) were measured in the cerebral cortex, hippocampus, and striatum by Western blotting. RESULTS: Compared to controls, the intermittent hypoxia group displayed less anxiety-like behavior, perceived by a statistically significant increase in the number of entries and total time spent in open arms. A higher expression of GR1 in the cortex was also observed. CONCLUSION: The lack of a clear anxiety response as an outcome of intermittent hypoxia exposure suggests the existence of additional layers in the anxiety mechanism in sleep apnea, possibly represented by sleepiness and irreversible neuronal damage.
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spelling pubmed-68993762019-12-30 Intermittent hypoxia, brain glyoxalase-1 and glutathione reductase-1, and anxiety-like behavior in mice Carissimi, Alicia Martinez, Denis Kim, Lenise J. Fiori, Cintia Z. Vieira, Luciana R. Rosa, Darlan P. Pires, Gabriel N. Braz J Psychiatry Original Article OBJECTIVE: Sleep apnea has been associated with anxiety, but the mechanisms of the sleep apnea-anxiety relationship are unresolved. Sleep apnea causes oxidative stress, which might enhance anxiety-like behavior in rodents. To clarify the apnea-anxiety connection, we tested the effect of intermittent hypoxia, a model of sleep apnea, on the anxiety behavior of mice. METHODS: The rodents were exposed daily to 480 one-minute cycles of intermittent hypoxia to a nadir of 7±1% inspiratory oxygen fraction or to a sham procedure with room air. After 7 days, the mice from both groups were placed in an elevated plus maze and were video recorded for 10 min to allow analysis of latency, frequency, and duration in open and closed arms. Glyoxalase-1 (Glo1) and glutathione reductase-1 (GR1) were measured in the cerebral cortex, hippocampus, and striatum by Western blotting. RESULTS: Compared to controls, the intermittent hypoxia group displayed less anxiety-like behavior, perceived by a statistically significant increase in the number of entries and total time spent in open arms. A higher expression of GR1 in the cortex was also observed. CONCLUSION: The lack of a clear anxiety response as an outcome of intermittent hypoxia exposure suggests the existence of additional layers in the anxiety mechanism in sleep apnea, possibly represented by sleepiness and irreversible neuronal damage. Associação Brasileira de Psiquiatria 2018-08-09 /pmc/articles/PMC6899376/ /pubmed/30110090 http://dx.doi.org/10.1590/1516-4446-2017-2310 Text en http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Carissimi, Alicia
Martinez, Denis
Kim, Lenise J.
Fiori, Cintia Z.
Vieira, Luciana R.
Rosa, Darlan P.
Pires, Gabriel N.
Intermittent hypoxia, brain glyoxalase-1 and glutathione reductase-1, and anxiety-like behavior in mice
title Intermittent hypoxia, brain glyoxalase-1 and glutathione reductase-1, and anxiety-like behavior in mice
title_full Intermittent hypoxia, brain glyoxalase-1 and glutathione reductase-1, and anxiety-like behavior in mice
title_fullStr Intermittent hypoxia, brain glyoxalase-1 and glutathione reductase-1, and anxiety-like behavior in mice
title_full_unstemmed Intermittent hypoxia, brain glyoxalase-1 and glutathione reductase-1, and anxiety-like behavior in mice
title_short Intermittent hypoxia, brain glyoxalase-1 and glutathione reductase-1, and anxiety-like behavior in mice
title_sort intermittent hypoxia, brain glyoxalase-1 and glutathione reductase-1, and anxiety-like behavior in mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6899376/
https://www.ncbi.nlm.nih.gov/pubmed/30110090
http://dx.doi.org/10.1590/1516-4446-2017-2310
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