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Inhibition of breast cancer growth via miR‐7 suppressing ALDH1A3 activity concomitant with decreasing breast cancer stem cell subpopulation

Breast cancer patients with high expression of aldehyde dehydrogenases (ALDHs) cell population have higher tolerability to chemotherapy since the cells posses a characteristic of breast cancer stem cells (BCSCs) that are resistant to conventional chemotherapy. In this study, we found that the ALDH‐p...

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Autores principales: Pan, Meng, Li, Miao, You, Chengzhong, Zhao, Fengshu, Guo, Mei, Xu, Hui, Li, Luoyang, Wang, Ling, Dou, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6899543/
https://www.ncbi.nlm.nih.gov/pubmed/31347176
http://dx.doi.org/10.1002/jcp.29059
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author Pan, Meng
Li, Miao
You, Chengzhong
Zhao, Fengshu
Guo, Mei
Xu, Hui
Li, Luoyang
Wang, Ling
Dou, Jun
author_facet Pan, Meng
Li, Miao
You, Chengzhong
Zhao, Fengshu
Guo, Mei
Xu, Hui
Li, Luoyang
Wang, Ling
Dou, Jun
author_sort Pan, Meng
collection PubMed
description Breast cancer patients with high expression of aldehyde dehydrogenases (ALDHs) cell population have higher tolerability to chemotherapy since the cells posses a characteristic of breast cancer stem cells (BCSCs) that are resistant to conventional chemotherapy. In this study, we found that the ALDH‐positive cells were higher in CD44(+)CD24(−) and CD44(+)CD24(−)ESA(+)BCSCs than that in both BT549 and MDA‐MB‐231 cell lines but microRNA‐7 (miR‐7) level was lower in CD44(+)CD24(−) and CD44(+)CD24(−)ESA(+)BCSCs than that in MDA‐MB‐231 cells. Moreover, miR‐7 overexpression in MDA‐MB‐231 cells decreased ALDH1A3 activity by miR‐7 directly binding to the 3′‐untranslated region of ALDH1A3; while the ALDH1A3 expression was downregulated in MDA‐MB‐231 cells, the expressions of CD44 and Epithelium Specific Antigen (ESA) were reduced along with decreasing the BCSC subpopulation. Significantly, enforced expression of miR‐7 in CD44(+)CD24(−)ESA(+)BCSC markedly inhibited the BCSC‐driven xenograft growth in mice by decreasing an expression of ALDH1A3. Collectively, the findings demonstrate the miR‐7 inhibits breast cancer growth via suppressing ALDH1A3 activity concomitant with decreasing BCSC subpopulation. This approach may be considered for an investigation on clinical treatment of breast cancers.
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spelling pubmed-68995432019-12-19 Inhibition of breast cancer growth via miR‐7 suppressing ALDH1A3 activity concomitant with decreasing breast cancer stem cell subpopulation Pan, Meng Li, Miao You, Chengzhong Zhao, Fengshu Guo, Mei Xu, Hui Li, Luoyang Wang, Ling Dou, Jun J Cell Physiol Original Research Articles Breast cancer patients with high expression of aldehyde dehydrogenases (ALDHs) cell population have higher tolerability to chemotherapy since the cells posses a characteristic of breast cancer stem cells (BCSCs) that are resistant to conventional chemotherapy. In this study, we found that the ALDH‐positive cells were higher in CD44(+)CD24(−) and CD44(+)CD24(−)ESA(+)BCSCs than that in both BT549 and MDA‐MB‐231 cell lines but microRNA‐7 (miR‐7) level was lower in CD44(+)CD24(−) and CD44(+)CD24(−)ESA(+)BCSCs than that in MDA‐MB‐231 cells. Moreover, miR‐7 overexpression in MDA‐MB‐231 cells decreased ALDH1A3 activity by miR‐7 directly binding to the 3′‐untranslated region of ALDH1A3; while the ALDH1A3 expression was downregulated in MDA‐MB‐231 cells, the expressions of CD44 and Epithelium Specific Antigen (ESA) were reduced along with decreasing the BCSC subpopulation. Significantly, enforced expression of miR‐7 in CD44(+)CD24(−)ESA(+)BCSC markedly inhibited the BCSC‐driven xenograft growth in mice by decreasing an expression of ALDH1A3. Collectively, the findings demonstrate the miR‐7 inhibits breast cancer growth via suppressing ALDH1A3 activity concomitant with decreasing BCSC subpopulation. This approach may be considered for an investigation on clinical treatment of breast cancers. John Wiley and Sons Inc. 2019-07-25 2020-02 /pmc/articles/PMC6899543/ /pubmed/31347176 http://dx.doi.org/10.1002/jcp.29059 Text en © 2019 The Authors Journal of Cellular Physiology Published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Research Articles
Pan, Meng
Li, Miao
You, Chengzhong
Zhao, Fengshu
Guo, Mei
Xu, Hui
Li, Luoyang
Wang, Ling
Dou, Jun
Inhibition of breast cancer growth via miR‐7 suppressing ALDH1A3 activity concomitant with decreasing breast cancer stem cell subpopulation
title Inhibition of breast cancer growth via miR‐7 suppressing ALDH1A3 activity concomitant with decreasing breast cancer stem cell subpopulation
title_full Inhibition of breast cancer growth via miR‐7 suppressing ALDH1A3 activity concomitant with decreasing breast cancer stem cell subpopulation
title_fullStr Inhibition of breast cancer growth via miR‐7 suppressing ALDH1A3 activity concomitant with decreasing breast cancer stem cell subpopulation
title_full_unstemmed Inhibition of breast cancer growth via miR‐7 suppressing ALDH1A3 activity concomitant with decreasing breast cancer stem cell subpopulation
title_short Inhibition of breast cancer growth via miR‐7 suppressing ALDH1A3 activity concomitant with decreasing breast cancer stem cell subpopulation
title_sort inhibition of breast cancer growth via mir‐7 suppressing aldh1a3 activity concomitant with decreasing breast cancer stem cell subpopulation
topic Original Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6899543/
https://www.ncbi.nlm.nih.gov/pubmed/31347176
http://dx.doi.org/10.1002/jcp.29059
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