Cargando…
RNF144B inhibits LPS‐induced inflammatory responses via binding TBK1
Innate immune responses need to be precisely controlled to avoid prolonged inflammation and prevent unwanted damage to the host. Here, we report that RNF144B responded dynamically to LPS stimulation and negatively regulated LPS‐induced inflammation. We found that RNF144B interacted with the scaffold...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6899866/ https://www.ncbi.nlm.nih.gov/pubmed/31509299 http://dx.doi.org/10.1002/JLB.2A0819-055R |
_version_ | 1783477226574970880 |
---|---|
author | Zhang, Zhen Zhang, Luoyan Wang, Bin Zhu, Xiaoxiao Zhao, Lin Chu, Chu Guo, Qiang Wei, Ran Yin, Xunqiang Zhang, Yunhong Li, Xia |
author_facet | Zhang, Zhen Zhang, Luoyan Wang, Bin Zhu, Xiaoxiao Zhao, Lin Chu, Chu Guo, Qiang Wei, Ran Yin, Xunqiang Zhang, Yunhong Li, Xia |
author_sort | Zhang, Zhen |
collection | PubMed |
description | Innate immune responses need to be precisely controlled to avoid prolonged inflammation and prevent unwanted damage to the host. Here, we report that RNF144B responded dynamically to LPS stimulation and negatively regulated LPS‐induced inflammation. We found that RNF144B interacted with the scaffold/dimerization domain (SDD) of TANK binding kinase 1 (TBK1) through the in between RING (IBR) domain to inhibit its phosphorylation and K63‐linked polyubiquitination, which led to TBK1 inactivation, IRF3 dephosphorylation, and IFN‐β reduction. RNF144B knockdown with siRNA increased IRF3 activation and IFN‐β production in response to LPS stimulation. Our study identifies that RNF144B interaction with TBK1 is sufficient to inactivate TBK1 and delineates a previously unrecognized role for RNF144B in innate immune responses. |
format | Online Article Text |
id | pubmed-6899866 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-68998662019-12-19 RNF144B inhibits LPS‐induced inflammatory responses via binding TBK1 Zhang, Zhen Zhang, Luoyan Wang, Bin Zhu, Xiaoxiao Zhao, Lin Chu, Chu Guo, Qiang Wei, Ran Yin, Xunqiang Zhang, Yunhong Li, Xia J Leukoc Biol Receptors, Signal Transduction, & Genes Innate immune responses need to be precisely controlled to avoid prolonged inflammation and prevent unwanted damage to the host. Here, we report that RNF144B responded dynamically to LPS stimulation and negatively regulated LPS‐induced inflammation. We found that RNF144B interacted with the scaffold/dimerization domain (SDD) of TANK binding kinase 1 (TBK1) through the in between RING (IBR) domain to inhibit its phosphorylation and K63‐linked polyubiquitination, which led to TBK1 inactivation, IRF3 dephosphorylation, and IFN‐β reduction. RNF144B knockdown with siRNA increased IRF3 activation and IFN‐β production in response to LPS stimulation. Our study identifies that RNF144B interaction with TBK1 is sufficient to inactivate TBK1 and delineates a previously unrecognized role for RNF144B in innate immune responses. John Wiley and Sons Inc. 2019-09-11 2019-12 /pmc/articles/PMC6899866/ /pubmed/31509299 http://dx.doi.org/10.1002/JLB.2A0819-055R Text en © 2019 The Authors. Journal of Leukocyte Biology published by Wiley Periodicals, Inc. on behalf of Society for Leukocyte Biology This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Receptors, Signal Transduction, & Genes Zhang, Zhen Zhang, Luoyan Wang, Bin Zhu, Xiaoxiao Zhao, Lin Chu, Chu Guo, Qiang Wei, Ran Yin, Xunqiang Zhang, Yunhong Li, Xia RNF144B inhibits LPS‐induced inflammatory responses via binding TBK1 |
title | RNF144B inhibits LPS‐induced inflammatory responses via binding TBK1 |
title_full | RNF144B inhibits LPS‐induced inflammatory responses via binding TBK1 |
title_fullStr | RNF144B inhibits LPS‐induced inflammatory responses via binding TBK1 |
title_full_unstemmed | RNF144B inhibits LPS‐induced inflammatory responses via binding TBK1 |
title_short | RNF144B inhibits LPS‐induced inflammatory responses via binding TBK1 |
title_sort | rnf144b inhibits lps‐induced inflammatory responses via binding tbk1 |
topic | Receptors, Signal Transduction, & Genes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6899866/ https://www.ncbi.nlm.nih.gov/pubmed/31509299 http://dx.doi.org/10.1002/JLB.2A0819-055R |
work_keys_str_mv | AT zhangzhen rnf144binhibitslpsinducedinflammatoryresponsesviabindingtbk1 AT zhangluoyan rnf144binhibitslpsinducedinflammatoryresponsesviabindingtbk1 AT wangbin rnf144binhibitslpsinducedinflammatoryresponsesviabindingtbk1 AT zhuxiaoxiao rnf144binhibitslpsinducedinflammatoryresponsesviabindingtbk1 AT zhaolin rnf144binhibitslpsinducedinflammatoryresponsesviabindingtbk1 AT chuchu rnf144binhibitslpsinducedinflammatoryresponsesviabindingtbk1 AT guoqiang rnf144binhibitslpsinducedinflammatoryresponsesviabindingtbk1 AT weiran rnf144binhibitslpsinducedinflammatoryresponsesviabindingtbk1 AT yinxunqiang rnf144binhibitslpsinducedinflammatoryresponsesviabindingtbk1 AT zhangyunhong rnf144binhibitslpsinducedinflammatoryresponsesviabindingtbk1 AT lixia rnf144binhibitslpsinducedinflammatoryresponsesviabindingtbk1 |