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Exosomes From Astrocyte Processes: Signaling to Neurons

It is widely recognized that extracellular vesicles subserve non-classical signal transmission in the central nervous system. Here we assess if the astrocyte processes, that are recognized to play crucial roles in intercellular communication at the synapses and in neuron-astrocyte networks, could co...

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Autores principales: Venturini, Arianna, Passalacqua, Mario, Pelassa, Simone, Pastorino, Fabio, Tedesco, Mariateresa, Cortese, Katia, Gagliani, Maria Cristina, Leo, Giuseppina, Maura, Guido, Guidolin, Diego, Agnati, Luigi F., Marcoli, Manuela, Cervetto, Chiara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6901013/
https://www.ncbi.nlm.nih.gov/pubmed/31849688
http://dx.doi.org/10.3389/fphar.2019.01452
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author Venturini, Arianna
Passalacqua, Mario
Pelassa, Simone
Pastorino, Fabio
Tedesco, Mariateresa
Cortese, Katia
Gagliani, Maria Cristina
Leo, Giuseppina
Maura, Guido
Guidolin, Diego
Agnati, Luigi F.
Marcoli, Manuela
Cervetto, Chiara
author_facet Venturini, Arianna
Passalacqua, Mario
Pelassa, Simone
Pastorino, Fabio
Tedesco, Mariateresa
Cortese, Katia
Gagliani, Maria Cristina
Leo, Giuseppina
Maura, Guido
Guidolin, Diego
Agnati, Luigi F.
Marcoli, Manuela
Cervetto, Chiara
author_sort Venturini, Arianna
collection PubMed
description It is widely recognized that extracellular vesicles subserve non-classical signal transmission in the central nervous system. Here we assess if the astrocyte processes, that are recognized to play crucial roles in intercellular communication at the synapses and in neuron-astrocyte networks, could convey messages through extracellular vesicles. Our findings indicate, for the first time that freshly isolated astrocyte processes prepared from adult rat cerebral cortex, can indeed participate to signal transmission in central nervous system by releasing exosomes that by volume transmission might target near or long-distance sites. It is noteworthy that the exosomes released from the astrocyte processes proved ability to selectively target neurons. The astrocyte-derived exosomes were proven positive for neuroglobin, a protein functioning as neuroprotectant against cell insult; the possibility that exosomes might transfer neuroglobin to neurons would add a mechanism to the potential astrocytic neuroprotectant activity. Notably, the exosomes released from the processes of astrocytes maintained markers, which prove their parental astrocytic origin. This potentially allows the assessment of the cellular origin of exosomes that might be recovered from body fluids.
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spelling pubmed-69010132019-12-17 Exosomes From Astrocyte Processes: Signaling to Neurons Venturini, Arianna Passalacqua, Mario Pelassa, Simone Pastorino, Fabio Tedesco, Mariateresa Cortese, Katia Gagliani, Maria Cristina Leo, Giuseppina Maura, Guido Guidolin, Diego Agnati, Luigi F. Marcoli, Manuela Cervetto, Chiara Front Pharmacol Pharmacology It is widely recognized that extracellular vesicles subserve non-classical signal transmission in the central nervous system. Here we assess if the astrocyte processes, that are recognized to play crucial roles in intercellular communication at the synapses and in neuron-astrocyte networks, could convey messages through extracellular vesicles. Our findings indicate, for the first time that freshly isolated astrocyte processes prepared from adult rat cerebral cortex, can indeed participate to signal transmission in central nervous system by releasing exosomes that by volume transmission might target near or long-distance sites. It is noteworthy that the exosomes released from the astrocyte processes proved ability to selectively target neurons. The astrocyte-derived exosomes were proven positive for neuroglobin, a protein functioning as neuroprotectant against cell insult; the possibility that exosomes might transfer neuroglobin to neurons would add a mechanism to the potential astrocytic neuroprotectant activity. Notably, the exosomes released from the processes of astrocytes maintained markers, which prove their parental astrocytic origin. This potentially allows the assessment of the cellular origin of exosomes that might be recovered from body fluids. Frontiers Media S.A. 2019-12-02 /pmc/articles/PMC6901013/ /pubmed/31849688 http://dx.doi.org/10.3389/fphar.2019.01452 Text en Copyright © 2019 Venturini, Passalacqua, Pelassa, Pastorino, Tedesco, Cortese, Gagliani, Leo, Maura, Guidolin, Agnati, Marcoli and Cervetto http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Venturini, Arianna
Passalacqua, Mario
Pelassa, Simone
Pastorino, Fabio
Tedesco, Mariateresa
Cortese, Katia
Gagliani, Maria Cristina
Leo, Giuseppina
Maura, Guido
Guidolin, Diego
Agnati, Luigi F.
Marcoli, Manuela
Cervetto, Chiara
Exosomes From Astrocyte Processes: Signaling to Neurons
title Exosomes From Astrocyte Processes: Signaling to Neurons
title_full Exosomes From Astrocyte Processes: Signaling to Neurons
title_fullStr Exosomes From Astrocyte Processes: Signaling to Neurons
title_full_unstemmed Exosomes From Astrocyte Processes: Signaling to Neurons
title_short Exosomes From Astrocyte Processes: Signaling to Neurons
title_sort exosomes from astrocyte processes: signaling to neurons
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6901013/
https://www.ncbi.nlm.nih.gov/pubmed/31849688
http://dx.doi.org/10.3389/fphar.2019.01452
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