The inability of a dengue NS1 ELISA to detect Zika infections

The presence of dengue virus (DENV), Zika virus (ZIKV) and Chikungunya virus (CHIKV) in Brazil, may result in a difficult diagnosis due to the signs and symptoms shared by those. Moreover, as DENV and ZIKV belong to the same family, serological assays may show a high rate of cross-reactivity. Here,...

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Detalles Bibliográficos
Autores principales: Lima, Monique da Rocha Queiroz, Chouin-Carneiro, Thais, Azeredo, Elzinandes, Barbosa, Luciana Santos, Souza, Thiara Manuele Alves, Silva, Jéssica Badolato Corrêa da, Nunes, Priscila Conrado Guerra, Dal Fabbro, Márcia, Facco, Izilyanne H. Romanholi, Venâncio-da-Cunha, Rivaldo, dos Santos, Flavia Barreto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6901494/
https://www.ncbi.nlm.nih.gov/pubmed/31819129
http://dx.doi.org/10.1038/s41598-019-55160-4
Descripción
Sumario:The presence of dengue virus (DENV), Zika virus (ZIKV) and Chikungunya virus (CHIKV) in Brazil, may result in a difficult diagnosis due to the signs and symptoms shared by those. Moreover, as DENV and ZIKV belong to the same family, serological assays may show a high rate of cross-reactivity. Here, we evaluated a Dengue NS1 capture assay for early and differential diagnosis of dengue during the Zika epidemic occurred in Brazil in 2016. Samples (n = 227) from 218 patients included sera, plasma and urine from previously confirmed acute cases of Zika, dengue and Zika/dengue co-infections. Nine of those patients presented two specimens. The Dengue NS1 test was very specific for dengue diagnosis (99.32%), even in the co-circulation with ZIKV, and exhibited a high accuracy in not detecting acute Zika infections (92.43%). Our findings showed that the dengue NS1 capture test analyzed here was not able to recognize the ZIKV NS1 and its potential for cross-reaction.

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