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Sucrose Starvation Induces Microautophagy in Plant Root Cells
Autophagy is an essential system for degrading and recycling cellular components for survival during starvation conditions. Under sucrose starvation, application of a papain protease inhibitor E-64d to the Arabidopsis root and tobacco BY-2 cells induced the accumulation of vesicles, labeled with a f...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6901504/ https://www.ncbi.nlm.nih.gov/pubmed/31850051 http://dx.doi.org/10.3389/fpls.2019.01604 |
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author | Goto-Yamada, Shino Oikawa, Kazusato Bizan, Jakub Shigenobu, Shuji Yamaguchi, Katsushi Mano, Shoji Hayashi, Makoto Ueda, Haruko Hara-Nishimura, Ikuko Nishimura, Mikio Yamada, Kenji |
author_facet | Goto-Yamada, Shino Oikawa, Kazusato Bizan, Jakub Shigenobu, Shuji Yamaguchi, Katsushi Mano, Shoji Hayashi, Makoto Ueda, Haruko Hara-Nishimura, Ikuko Nishimura, Mikio Yamada, Kenji |
author_sort | Goto-Yamada, Shino |
collection | PubMed |
description | Autophagy is an essential system for degrading and recycling cellular components for survival during starvation conditions. Under sucrose starvation, application of a papain protease inhibitor E-64d to the Arabidopsis root and tobacco BY-2 cells induced the accumulation of vesicles, labeled with a fluorescent membrane marker FM4-64. The E-64d–induced vesicle accumulation was reduced in the mutant defective in autophagy-related genes ATG2, ATG5, and ATG7, suggesting autophagy is involved in the formation of these vesicles. To clarify the formation of these vesicles in detail, we monitored time-dependent changes of tonoplast, and vesicle accumulation in sucrose-starved cells. We found that these vesicles were derived from the tonoplast and produced by microautophagic process. The tonoplast proteins were excluded from the vesicles, suggesting that the vesicles are generated from specific membrane domains. Concanamycin A treatment in GFP-ATG8a transgenic plants showed that not all FM4-64–labeled vesicles, which were derived from the tonoplast, contained the ATG8a-containing structure. These results suggest that ATG8a may not always be necessary for microautophagy. |
format | Online Article Text |
id | pubmed-6901504 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69015042019-12-17 Sucrose Starvation Induces Microautophagy in Plant Root Cells Goto-Yamada, Shino Oikawa, Kazusato Bizan, Jakub Shigenobu, Shuji Yamaguchi, Katsushi Mano, Shoji Hayashi, Makoto Ueda, Haruko Hara-Nishimura, Ikuko Nishimura, Mikio Yamada, Kenji Front Plant Sci Plant Science Autophagy is an essential system for degrading and recycling cellular components for survival during starvation conditions. Under sucrose starvation, application of a papain protease inhibitor E-64d to the Arabidopsis root and tobacco BY-2 cells induced the accumulation of vesicles, labeled with a fluorescent membrane marker FM4-64. The E-64d–induced vesicle accumulation was reduced in the mutant defective in autophagy-related genes ATG2, ATG5, and ATG7, suggesting autophagy is involved in the formation of these vesicles. To clarify the formation of these vesicles in detail, we monitored time-dependent changes of tonoplast, and vesicle accumulation in sucrose-starved cells. We found that these vesicles were derived from the tonoplast and produced by microautophagic process. The tonoplast proteins were excluded from the vesicles, suggesting that the vesicles are generated from specific membrane domains. Concanamycin A treatment in GFP-ATG8a transgenic plants showed that not all FM4-64–labeled vesicles, which were derived from the tonoplast, contained the ATG8a-containing structure. These results suggest that ATG8a may not always be necessary for microautophagy. Frontiers Media S.A. 2019-12-03 /pmc/articles/PMC6901504/ /pubmed/31850051 http://dx.doi.org/10.3389/fpls.2019.01604 Text en Copyright © 2019 Goto-Yamada, Oikawa, Bizan, Shigenobu, Yamaguchi, Mano, Hayashi, Ueda, Hara-Nishimura, Nishimura and Yamada http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Goto-Yamada, Shino Oikawa, Kazusato Bizan, Jakub Shigenobu, Shuji Yamaguchi, Katsushi Mano, Shoji Hayashi, Makoto Ueda, Haruko Hara-Nishimura, Ikuko Nishimura, Mikio Yamada, Kenji Sucrose Starvation Induces Microautophagy in Plant Root Cells |
title | Sucrose Starvation Induces Microautophagy in Plant Root Cells |
title_full | Sucrose Starvation Induces Microautophagy in Plant Root Cells |
title_fullStr | Sucrose Starvation Induces Microautophagy in Plant Root Cells |
title_full_unstemmed | Sucrose Starvation Induces Microautophagy in Plant Root Cells |
title_short | Sucrose Starvation Induces Microautophagy in Plant Root Cells |
title_sort | sucrose starvation induces microautophagy in plant root cells |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6901504/ https://www.ncbi.nlm.nih.gov/pubmed/31850051 http://dx.doi.org/10.3389/fpls.2019.01604 |
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