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Coupling chemical mutagenesis to next generation sequencing for the identification of drug resistance mutations in Leishmania

Current genome-wide screens allow system-wide study of drug resistance but detecting small nucleotide variants (SNVs) is challenging. Here, we use chemical mutagenesis, drug selection and next generation sequencing to characterize miltefosine and paromomycin resistant clones of the parasite Leishman...

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Detalles Bibliográficos
Autores principales:	Bhattacharya, Arijit, Leprohon, Philippe, Bigot, Sophia, Padmanabhan, Prasad Kottayil, Mukherjee, Angana, Roy, Gaétan, Gingras, Hélène, Mestdagh, Anais, Papadopoulou, Barbara, Ouellette, Marc
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6901541/ https://www.ncbi.nlm.nih.gov/pubmed/31819054 http://dx.doi.org/10.1038/s41467-019-13344-6

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