Cargando…
Hypertension Associated With Hyperlipidemia Induced Different MicroRNA Expression Profiles in Plasma, Platelets, and Platelet-Derived Microvesicles; Effects of Endothelial Progenitor Cell Therapy
Aim: The aim of this study was to analyze the expressed profiles of miRNAs in plasma, platelets, and platelet-derived microvesicles (PMVs) obtained from experimental induced atherosclerosis animal model and to investigate the effect of EPC transplantation on these profiles. Methods: Seventeen select...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6901790/ https://www.ncbi.nlm.nih.gov/pubmed/31850358 http://dx.doi.org/10.3389/fmed.2019.00280 |
_version_ | 1783477561874972672 |
---|---|
author | Alexandru, Nicoleta Constantin, Alina Nemecz, Miruna Comariţa, Ioana Karla Vîlcu, Alexandra Procopciuc, Anastasia Tanko, Gabriela Georgescu, Adriana |
author_facet | Alexandru, Nicoleta Constantin, Alina Nemecz, Miruna Comariţa, Ioana Karla Vîlcu, Alexandra Procopciuc, Anastasia Tanko, Gabriela Georgescu, Adriana |
author_sort | Alexandru, Nicoleta |
collection | PubMed |
description | Aim: The aim of this study was to analyze the expressed profiles of miRNAs in plasma, platelets, and platelet-derived microvesicles (PMVs) obtained from experimental induced atherosclerosis animal model and to investigate the effect of EPC transplantation on these profiles. Methods: Seventeen selected circulating miRNAs (miR-19a,-21,-126,-146a,-223,-26b,-92a,-222,-210,-221,-143,-10a,-145,-155,-34a,-204, and miR-214) were individually analyzed in plasma, platelets, and PMVs isolated from peripheral blood of hypertensive-hyperlipidemic hamsters treated or not with endothelial progenitor cells (EPCs), and of healthy hamsters taken as control group. Results: Comparative with control group, in hypertension associated with hyperlipidemia the investigated miRNA expression profiles were different: (i) in plasma, the levels of all investigated miRNAs were significantly increased, the highest enhances being noticed for miR-21,-146a,-221,-143,-34a, and miR-204; (ii) in platelets, the expressions of almost all miRNAs were significantly elevated, remarkable for miR-126,-146a,-223,-222, and miR-214, while levels of miR-143, miR-10a, and miR-145 were significantly reduced; (iii) in PMVs, numerous miRNAs were found to have significantly increased levels, especially miR-222,-221,-210, and miR-34a, whereas expressions of various miRNAs as miR-223,-214,-146a,-143,-10a, and miR-145 were significantly decreased. The treatment with EPCs had the following reverse effects: (i) in plasma, significantly reduced the expression of miR-26b,-143,-34a,-204, and miR-214; (ii) in platelets, significantly decreased the levels of almost investigated miRNAs, with remarkably diminishing for miR-126 and miR-221; and (iii) in PMVs, significantly lowered the expression of some miRNAs, with considerably reductions for miR-222,-221,-210, and miR-19a, while the level of miR-214 was found elevated. Conclusions: The present study revealed that miRNAs have differential expression profiles in plasma, platelets, and PMVs under hypertension associated with hyperlipidemia conditions. The different miRNA profile in PMVs compared with platelets indicated an active mechanism of selective packing of miRNAs into PMVs from maternal cells; various miRNAs such as miR-19a,-21,-126,-26b,-92a,-155,-204,-210,-221,-222, and−34a delivered by PMVs may contribute to enrichment of circulating plasma miRNA expression. In addition, our study showed that the EPC-based therapy can regulate the expressions of investigated miRNAs into the three sources. These results provide novel information that could help in finding potential targets for the development of new therapeutic strategies in the cardiovascular disease. |
format | Online Article Text |
id | pubmed-6901790 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69017902019-12-17 Hypertension Associated With Hyperlipidemia Induced Different MicroRNA Expression Profiles in Plasma, Platelets, and Platelet-Derived Microvesicles; Effects of Endothelial Progenitor Cell Therapy Alexandru, Nicoleta Constantin, Alina Nemecz, Miruna Comariţa, Ioana Karla Vîlcu, Alexandra Procopciuc, Anastasia Tanko, Gabriela Georgescu, Adriana Front Med (Lausanne) Medicine Aim: The aim of this study was to analyze the expressed profiles of miRNAs in plasma, platelets, and platelet-derived microvesicles (PMVs) obtained from experimental induced atherosclerosis animal model and to investigate the effect of EPC transplantation on these profiles. Methods: Seventeen selected circulating miRNAs (miR-19a,-21,-126,-146a,-223,-26b,-92a,-222,-210,-221,-143,-10a,-145,-155,-34a,-204, and miR-214) were individually analyzed in plasma, platelets, and PMVs isolated from peripheral blood of hypertensive-hyperlipidemic hamsters treated or not with endothelial progenitor cells (EPCs), and of healthy hamsters taken as control group. Results: Comparative with control group, in hypertension associated with hyperlipidemia the investigated miRNA expression profiles were different: (i) in plasma, the levels of all investigated miRNAs were significantly increased, the highest enhances being noticed for miR-21,-146a,-221,-143,-34a, and miR-204; (ii) in platelets, the expressions of almost all miRNAs were significantly elevated, remarkable for miR-126,-146a,-223,-222, and miR-214, while levels of miR-143, miR-10a, and miR-145 were significantly reduced; (iii) in PMVs, numerous miRNAs were found to have significantly increased levels, especially miR-222,-221,-210, and miR-34a, whereas expressions of various miRNAs as miR-223,-214,-146a,-143,-10a, and miR-145 were significantly decreased. The treatment with EPCs had the following reverse effects: (i) in plasma, significantly reduced the expression of miR-26b,-143,-34a,-204, and miR-214; (ii) in platelets, significantly decreased the levels of almost investigated miRNAs, with remarkably diminishing for miR-126 and miR-221; and (iii) in PMVs, significantly lowered the expression of some miRNAs, with considerably reductions for miR-222,-221,-210, and miR-19a, while the level of miR-214 was found elevated. Conclusions: The present study revealed that miRNAs have differential expression profiles in plasma, platelets, and PMVs under hypertension associated with hyperlipidemia conditions. The different miRNA profile in PMVs compared with platelets indicated an active mechanism of selective packing of miRNAs into PMVs from maternal cells; various miRNAs such as miR-19a,-21,-126,-26b,-92a,-155,-204,-210,-221,-222, and−34a delivered by PMVs may contribute to enrichment of circulating plasma miRNA expression. In addition, our study showed that the EPC-based therapy can regulate the expressions of investigated miRNAs into the three sources. These results provide novel information that could help in finding potential targets for the development of new therapeutic strategies in the cardiovascular disease. Frontiers Media S.A. 2019-12-03 /pmc/articles/PMC6901790/ /pubmed/31850358 http://dx.doi.org/10.3389/fmed.2019.00280 Text en Copyright © 2019 Alexandru, Constantin, Nemecz, Comariţa, Vîlcu, Procopciuc, Tanko and Georgescu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Medicine Alexandru, Nicoleta Constantin, Alina Nemecz, Miruna Comariţa, Ioana Karla Vîlcu, Alexandra Procopciuc, Anastasia Tanko, Gabriela Georgescu, Adriana Hypertension Associated With Hyperlipidemia Induced Different MicroRNA Expression Profiles in Plasma, Platelets, and Platelet-Derived Microvesicles; Effects of Endothelial Progenitor Cell Therapy |
title | Hypertension Associated With Hyperlipidemia Induced Different MicroRNA Expression Profiles in Plasma, Platelets, and Platelet-Derived Microvesicles; Effects of Endothelial Progenitor Cell Therapy |
title_full | Hypertension Associated With Hyperlipidemia Induced Different MicroRNA Expression Profiles in Plasma, Platelets, and Platelet-Derived Microvesicles; Effects of Endothelial Progenitor Cell Therapy |
title_fullStr | Hypertension Associated With Hyperlipidemia Induced Different MicroRNA Expression Profiles in Plasma, Platelets, and Platelet-Derived Microvesicles; Effects of Endothelial Progenitor Cell Therapy |
title_full_unstemmed | Hypertension Associated With Hyperlipidemia Induced Different MicroRNA Expression Profiles in Plasma, Platelets, and Platelet-Derived Microvesicles; Effects of Endothelial Progenitor Cell Therapy |
title_short | Hypertension Associated With Hyperlipidemia Induced Different MicroRNA Expression Profiles in Plasma, Platelets, and Platelet-Derived Microvesicles; Effects of Endothelial Progenitor Cell Therapy |
title_sort | hypertension associated with hyperlipidemia induced different microrna expression profiles in plasma, platelets, and platelet-derived microvesicles; effects of endothelial progenitor cell therapy |
topic | Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6901790/ https://www.ncbi.nlm.nih.gov/pubmed/31850358 http://dx.doi.org/10.3389/fmed.2019.00280 |
work_keys_str_mv | AT alexandrunicoleta hypertensionassociatedwithhyperlipidemiainduceddifferentmicrornaexpressionprofilesinplasmaplateletsandplateletderivedmicrovesicleseffectsofendothelialprogenitorcelltherapy AT constantinalina hypertensionassociatedwithhyperlipidemiainduceddifferentmicrornaexpressionprofilesinplasmaplateletsandplateletderivedmicrovesicleseffectsofendothelialprogenitorcelltherapy AT nemeczmiruna hypertensionassociatedwithhyperlipidemiainduceddifferentmicrornaexpressionprofilesinplasmaplateletsandplateletderivedmicrovesicleseffectsofendothelialprogenitorcelltherapy AT comaritaioanakarla hypertensionassociatedwithhyperlipidemiainduceddifferentmicrornaexpressionprofilesinplasmaplateletsandplateletderivedmicrovesicleseffectsofendothelialprogenitorcelltherapy AT vilcualexandra hypertensionassociatedwithhyperlipidemiainduceddifferentmicrornaexpressionprofilesinplasmaplateletsandplateletderivedmicrovesicleseffectsofendothelialprogenitorcelltherapy AT procopciucanastasia hypertensionassociatedwithhyperlipidemiainduceddifferentmicrornaexpressionprofilesinplasmaplateletsandplateletderivedmicrovesicleseffectsofendothelialprogenitorcelltherapy AT tankogabriela hypertensionassociatedwithhyperlipidemiainduceddifferentmicrornaexpressionprofilesinplasmaplateletsandplateletderivedmicrovesicleseffectsofendothelialprogenitorcelltherapy AT georgescuadriana hypertensionassociatedwithhyperlipidemiainduceddifferentmicrornaexpressionprofilesinplasmaplateletsandplateletderivedmicrovesicleseffectsofendothelialprogenitorcelltherapy |