Cargando…

Dogs as New Hosts for the Emerging Zoonotic Pathogen Anaplasma capra in China

Anaplasma capra is an emerging zoonotic tick-borne pathogen with a broad host range, including many mammals. Dogs have close physical interactions with humans and regular contact with the external environment. Moreover, they have been previously reported to be hosts of Anaplasma phagocytophilum, A....

Descripción completa

Detalles Bibliográficos
Autores principales: Shi, Ke, Li, Junqiang, Yan, Yaqun, Chen, Qian, Wang, Kunlun, Zhou, Yongchun, Li, Dongfang, Chen, Yuancai, Yu, Fuchang, Peng, Yongshuai, Zhang, Longxian, Ning, Changshen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6901931/
https://www.ncbi.nlm.nih.gov/pubmed/31850236
http://dx.doi.org/10.3389/fcimb.2019.00394
_version_ 1783477586148458496
author Shi, Ke
Li, Junqiang
Yan, Yaqun
Chen, Qian
Wang, Kunlun
Zhou, Yongchun
Li, Dongfang
Chen, Yuancai
Yu, Fuchang
Peng, Yongshuai
Zhang, Longxian
Ning, Changshen
author_facet Shi, Ke
Li, Junqiang
Yan, Yaqun
Chen, Qian
Wang, Kunlun
Zhou, Yongchun
Li, Dongfang
Chen, Yuancai
Yu, Fuchang
Peng, Yongshuai
Zhang, Longxian
Ning, Changshen
author_sort Shi, Ke
collection PubMed
description Anaplasma capra is an emerging zoonotic tick-borne pathogen with a broad host range, including many mammals. Dogs have close physical interactions with humans and regular contact with the external environment. Moreover, they have been previously reported to be hosts of Anaplasma phagocytophilum, A. platys, A. ovis, and A. bovis. To confirm whether dogs are also hosts of A. capra, pathogen DNA was extracted from blood samples of 521 dogs, followed by PCR amplification of the citrate synthase (gltA) gene, heat shock protein (groEL) gene, and major surface protein 4 (msp4) gene of the A. capra. A total of 12.1% (63/521) of blood samples were shown to be A. capra-positive by PCR screening. No significant differences were observed between genders (P = 0.578) or types (P = 0.154) of dogs with A. capra infections. However, significantly higher A. capra infections occurred in dogs with regular contact with vegetation (P = 0.002), those aged over 10 years (P = 0.040), and during the summer season (P = 0.006). Phylogenetic analysis based on gltA, groEL, and msp4 sequences demonstrated that the isolates obtained in this study were clustered within the A. capra clade, and were distinct from other Anaplasma species. In conclusion, dogs were shown to be a host of the human pathogenic A. capra. Considering the affinity between dogs and humans and the zoonotic tick-borne nature of A. capra, dogs should be carefully monitored for the presence of A. capra.
format Online
Article
Text
id pubmed-6901931
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-69019312019-12-17 Dogs as New Hosts for the Emerging Zoonotic Pathogen Anaplasma capra in China Shi, Ke Li, Junqiang Yan, Yaqun Chen, Qian Wang, Kunlun Zhou, Yongchun Li, Dongfang Chen, Yuancai Yu, Fuchang Peng, Yongshuai Zhang, Longxian Ning, Changshen Front Cell Infect Microbiol Cellular and Infection Microbiology Anaplasma capra is an emerging zoonotic tick-borne pathogen with a broad host range, including many mammals. Dogs have close physical interactions with humans and regular contact with the external environment. Moreover, they have been previously reported to be hosts of Anaplasma phagocytophilum, A. platys, A. ovis, and A. bovis. To confirm whether dogs are also hosts of A. capra, pathogen DNA was extracted from blood samples of 521 dogs, followed by PCR amplification of the citrate synthase (gltA) gene, heat shock protein (groEL) gene, and major surface protein 4 (msp4) gene of the A. capra. A total of 12.1% (63/521) of blood samples were shown to be A. capra-positive by PCR screening. No significant differences were observed between genders (P = 0.578) or types (P = 0.154) of dogs with A. capra infections. However, significantly higher A. capra infections occurred in dogs with regular contact with vegetation (P = 0.002), those aged over 10 years (P = 0.040), and during the summer season (P = 0.006). Phylogenetic analysis based on gltA, groEL, and msp4 sequences demonstrated that the isolates obtained in this study were clustered within the A. capra clade, and were distinct from other Anaplasma species. In conclusion, dogs were shown to be a host of the human pathogenic A. capra. Considering the affinity between dogs and humans and the zoonotic tick-borne nature of A. capra, dogs should be carefully monitored for the presence of A. capra. Frontiers Media S.A. 2019-11-26 /pmc/articles/PMC6901931/ /pubmed/31850236 http://dx.doi.org/10.3389/fcimb.2019.00394 Text en Copyright © 2019 Shi, Li, Yan, Chen, Wang, Zhou, Li, Chen, Yu, Peng, Zhang and Ning. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Shi, Ke
Li, Junqiang
Yan, Yaqun
Chen, Qian
Wang, Kunlun
Zhou, Yongchun
Li, Dongfang
Chen, Yuancai
Yu, Fuchang
Peng, Yongshuai
Zhang, Longxian
Ning, Changshen
Dogs as New Hosts for the Emerging Zoonotic Pathogen Anaplasma capra in China
title Dogs as New Hosts for the Emerging Zoonotic Pathogen Anaplasma capra in China
title_full Dogs as New Hosts for the Emerging Zoonotic Pathogen Anaplasma capra in China
title_fullStr Dogs as New Hosts for the Emerging Zoonotic Pathogen Anaplasma capra in China
title_full_unstemmed Dogs as New Hosts for the Emerging Zoonotic Pathogen Anaplasma capra in China
title_short Dogs as New Hosts for the Emerging Zoonotic Pathogen Anaplasma capra in China
title_sort dogs as new hosts for the emerging zoonotic pathogen anaplasma capra in china
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6901931/
https://www.ncbi.nlm.nih.gov/pubmed/31850236
http://dx.doi.org/10.3389/fcimb.2019.00394
work_keys_str_mv AT shike dogsasnewhostsfortheemergingzoonoticpathogenanaplasmacaprainchina
AT lijunqiang dogsasnewhostsfortheemergingzoonoticpathogenanaplasmacaprainchina
AT yanyaqun dogsasnewhostsfortheemergingzoonoticpathogenanaplasmacaprainchina
AT chenqian dogsasnewhostsfortheemergingzoonoticpathogenanaplasmacaprainchina
AT wangkunlun dogsasnewhostsfortheemergingzoonoticpathogenanaplasmacaprainchina
AT zhouyongchun dogsasnewhostsfortheemergingzoonoticpathogenanaplasmacaprainchina
AT lidongfang dogsasnewhostsfortheemergingzoonoticpathogenanaplasmacaprainchina
AT chenyuancai dogsasnewhostsfortheemergingzoonoticpathogenanaplasmacaprainchina
AT yufuchang dogsasnewhostsfortheemergingzoonoticpathogenanaplasmacaprainchina
AT pengyongshuai dogsasnewhostsfortheemergingzoonoticpathogenanaplasmacaprainchina
AT zhanglongxian dogsasnewhostsfortheemergingzoonoticpathogenanaplasmacaprainchina
AT ningchangshen dogsasnewhostsfortheemergingzoonoticpathogenanaplasmacaprainchina