Cargando…

Transcriptome and microbiome of coconut rhinoceros beetle (Oryctes rhinoceros) larvae

BACKGROUND: The coconut rhinoceros beetle, Oryctes rhinoceros, is a major pest of palm crops in tropical Asia and the Pacific Islands. Little molecular data exists for this pest, impeding our ability to develop effective countermeasures and deal with the species’ growing resistance to viral biocontr...

Descripción completa

Detalles Bibliográficos
Autores principales: Shelomi, Matan, Lin, Shih-Shun, Liu, Li-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6902462/
https://www.ncbi.nlm.nih.gov/pubmed/31818246
http://dx.doi.org/10.1186/s12864-019-6352-3
_version_ 1783477672368668672
author Shelomi, Matan
Lin, Shih-Shun
Liu, Li-Yu
author_facet Shelomi, Matan
Lin, Shih-Shun
Liu, Li-Yu
author_sort Shelomi, Matan
collection PubMed
description BACKGROUND: The coconut rhinoceros beetle, Oryctes rhinoceros, is a major pest of palm crops in tropical Asia and the Pacific Islands. Little molecular data exists for this pest, impeding our ability to develop effective countermeasures and deal with the species’ growing resistance to viral biocontrols. We present the first molecular biology analyses of this species, including a metagenomic assay to understand the microbiome of different sections of its digestive tract, and a transcriptomics assay to complement the microbiome data and to shed light on genes of interest like plant cell wall degrading enzymes and immunity and xenobiotic resistance genes. RESULTS: The gut microbiota of Oryctes rhinoceros larvae is quite similar to that of the termite gut, as both species feed on decaying wood. We found the first evidence for endogenous beta-1,4-endoglucanase in the beetle, plus evidence for microbial cellobiase, suggesting the beetle can degrade cellulose together with its gut microfauna. A number of antimicrobial peptides are expressed, particularly by the fat body but also by the midgut and hindgut. CONCLUSIONS: This transcriptome provides a wealth of data about the species’ defense against chemical and biological threats, has uncovered several potentially new species of microbial symbionts, and significantly expands our knowledge about this pest.
format Online
Article
Text
id pubmed-6902462
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-69024622019-12-11 Transcriptome and microbiome of coconut rhinoceros beetle (Oryctes rhinoceros) larvae Shelomi, Matan Lin, Shih-Shun Liu, Li-Yu BMC Genomics Research Article BACKGROUND: The coconut rhinoceros beetle, Oryctes rhinoceros, is a major pest of palm crops in tropical Asia and the Pacific Islands. Little molecular data exists for this pest, impeding our ability to develop effective countermeasures and deal with the species’ growing resistance to viral biocontrols. We present the first molecular biology analyses of this species, including a metagenomic assay to understand the microbiome of different sections of its digestive tract, and a transcriptomics assay to complement the microbiome data and to shed light on genes of interest like plant cell wall degrading enzymes and immunity and xenobiotic resistance genes. RESULTS: The gut microbiota of Oryctes rhinoceros larvae is quite similar to that of the termite gut, as both species feed on decaying wood. We found the first evidence for endogenous beta-1,4-endoglucanase in the beetle, plus evidence for microbial cellobiase, suggesting the beetle can degrade cellulose together with its gut microfauna. A number of antimicrobial peptides are expressed, particularly by the fat body but also by the midgut and hindgut. CONCLUSIONS: This transcriptome provides a wealth of data about the species’ defense against chemical and biological threats, has uncovered several potentially new species of microbial symbionts, and significantly expands our knowledge about this pest. BioMed Central 2019-12-09 /pmc/articles/PMC6902462/ /pubmed/31818246 http://dx.doi.org/10.1186/s12864-019-6352-3 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Shelomi, Matan
Lin, Shih-Shun
Liu, Li-Yu
Transcriptome and microbiome of coconut rhinoceros beetle (Oryctes rhinoceros) larvae
title Transcriptome and microbiome of coconut rhinoceros beetle (Oryctes rhinoceros) larvae
title_full Transcriptome and microbiome of coconut rhinoceros beetle (Oryctes rhinoceros) larvae
title_fullStr Transcriptome and microbiome of coconut rhinoceros beetle (Oryctes rhinoceros) larvae
title_full_unstemmed Transcriptome and microbiome of coconut rhinoceros beetle (Oryctes rhinoceros) larvae
title_short Transcriptome and microbiome of coconut rhinoceros beetle (Oryctes rhinoceros) larvae
title_sort transcriptome and microbiome of coconut rhinoceros beetle (oryctes rhinoceros) larvae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6902462/
https://www.ncbi.nlm.nih.gov/pubmed/31818246
http://dx.doi.org/10.1186/s12864-019-6352-3
work_keys_str_mv AT shelomimatan transcriptomeandmicrobiomeofcoconutrhinocerosbeetleoryctesrhinoceroslarvae
AT linshihshun transcriptomeandmicrobiomeofcoconutrhinocerosbeetleoryctesrhinoceroslarvae
AT liuliyu transcriptomeandmicrobiomeofcoconutrhinocerosbeetleoryctesrhinoceroslarvae