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Biosafety and biosecurity requirements for Orientia spp. diagnosis and research: recommendations for risk-based biocontainment, work practices and the case for reclassification to risk group 2
Scrub typhus is an important arthropod-borne disease causing significant acute febrile illness by infection with Orientia spp. Using a risk-based approach, this review examines current practice, the evidence base and regulatory requirements regarding matters of biosafety and biosecurity, and present...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6902544/ https://www.ncbi.nlm.nih.gov/pubmed/31822267 http://dx.doi.org/10.1186/s12879-019-4653-4 |
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author | Blacksell, Stuart D. Robinson, Matthew T. Newton, Paul N. Ruanchaimun, Soiratchaneekorn Salje, Jeanne Wangrangsimakul, Tri Wegner, Matthew D. Abdad, Mohammad Yazid Bennett, Allan M. Richards, Allen L. Stenos, John Day, Nicholas P. J. |
author_facet | Blacksell, Stuart D. Robinson, Matthew T. Newton, Paul N. Ruanchaimun, Soiratchaneekorn Salje, Jeanne Wangrangsimakul, Tri Wegner, Matthew D. Abdad, Mohammad Yazid Bennett, Allan M. Richards, Allen L. Stenos, John Day, Nicholas P. J. |
author_sort | Blacksell, Stuart D. |
collection | PubMed |
description | Scrub typhus is an important arthropod-borne disease causing significant acute febrile illness by infection with Orientia spp. Using a risk-based approach, this review examines current practice, the evidence base and regulatory requirements regarding matters of biosafety and biosecurity, and presents the case for reclassification from Risk Group 3 to Risk Group 2 along with recommendations for safe working practices of risk-based activities during the manipulation of Orientia spp. in the laboratory. We recommend to reclassify Orientia spp. to Risk Group 2 based on the classification for RG2 pathogens as being moderate individual risk, low community risk. We recommend that low risk activities, can be performed within a biological safety cabinet located in a Biosafety Level (BSL) 2 core laboratory using standard personal protective equipment. But when the risk assessment indicates, such as high concentration and volume, or aerosol generation, then a higher biocontainment level is warranted. For, the majority of animal activities involving Orientia spp., Animal BSL 2 (ABSL2) is recommended however where high risk activities are performed including necropsies, Animal BSL (ABSL3) is recommended. |
format | Online Article Text |
id | pubmed-6902544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-69025442019-12-11 Biosafety and biosecurity requirements for Orientia spp. diagnosis and research: recommendations for risk-based biocontainment, work practices and the case for reclassification to risk group 2 Blacksell, Stuart D. Robinson, Matthew T. Newton, Paul N. Ruanchaimun, Soiratchaneekorn Salje, Jeanne Wangrangsimakul, Tri Wegner, Matthew D. Abdad, Mohammad Yazid Bennett, Allan M. Richards, Allen L. Stenos, John Day, Nicholas P. J. BMC Infect Dis Debate Scrub typhus is an important arthropod-borne disease causing significant acute febrile illness by infection with Orientia spp. Using a risk-based approach, this review examines current practice, the evidence base and regulatory requirements regarding matters of biosafety and biosecurity, and presents the case for reclassification from Risk Group 3 to Risk Group 2 along with recommendations for safe working practices of risk-based activities during the manipulation of Orientia spp. in the laboratory. We recommend to reclassify Orientia spp. to Risk Group 2 based on the classification for RG2 pathogens as being moderate individual risk, low community risk. We recommend that low risk activities, can be performed within a biological safety cabinet located in a Biosafety Level (BSL) 2 core laboratory using standard personal protective equipment. But when the risk assessment indicates, such as high concentration and volume, or aerosol generation, then a higher biocontainment level is warranted. For, the majority of animal activities involving Orientia spp., Animal BSL 2 (ABSL2) is recommended however where high risk activities are performed including necropsies, Animal BSL (ABSL3) is recommended. BioMed Central 2019-12-10 /pmc/articles/PMC6902544/ /pubmed/31822267 http://dx.doi.org/10.1186/s12879-019-4653-4 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Debate Blacksell, Stuart D. Robinson, Matthew T. Newton, Paul N. Ruanchaimun, Soiratchaneekorn Salje, Jeanne Wangrangsimakul, Tri Wegner, Matthew D. Abdad, Mohammad Yazid Bennett, Allan M. Richards, Allen L. Stenos, John Day, Nicholas P. J. Biosafety and biosecurity requirements for Orientia spp. diagnosis and research: recommendations for risk-based biocontainment, work practices and the case for reclassification to risk group 2 |
title | Biosafety and biosecurity requirements for Orientia spp. diagnosis and research: recommendations for risk-based biocontainment, work practices and the case for reclassification to risk group 2 |
title_full | Biosafety and biosecurity requirements for Orientia spp. diagnosis and research: recommendations for risk-based biocontainment, work practices and the case for reclassification to risk group 2 |
title_fullStr | Biosafety and biosecurity requirements for Orientia spp. diagnosis and research: recommendations for risk-based biocontainment, work practices and the case for reclassification to risk group 2 |
title_full_unstemmed | Biosafety and biosecurity requirements for Orientia spp. diagnosis and research: recommendations for risk-based biocontainment, work practices and the case for reclassification to risk group 2 |
title_short | Biosafety and biosecurity requirements for Orientia spp. diagnosis and research: recommendations for risk-based biocontainment, work practices and the case for reclassification to risk group 2 |
title_sort | biosafety and biosecurity requirements for orientia spp. diagnosis and research: recommendations for risk-based biocontainment, work practices and the case for reclassification to risk group 2 |
topic | Debate |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6902544/ https://www.ncbi.nlm.nih.gov/pubmed/31822267 http://dx.doi.org/10.1186/s12879-019-4653-4 |
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