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Genetic analysis of a white-to-red berry skin color reversion and its transcriptomic and metabolic consequences in grapevine (Vitis vinifera cv. ‘Moscatel Galego’)

BACKGROUND: Somatic mutations occurring within meristems of vegetative propagation material have had a major role in increasing the genetic diversity of the domesticated grapevine (Vitis vinifera subsp. vinifera). The most well studied somatic variation in this species is the one affecting fruit pig...

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Autores principales: Ferreira, Vanessa, Matus, José Tomás, Pinto-Carnide, Olinda, Carrasco, David, Arroyo-García, Rosa, Castro, Isaura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6902604/
https://www.ncbi.nlm.nih.gov/pubmed/31815637
http://dx.doi.org/10.1186/s12864-019-6237-5
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author Ferreira, Vanessa
Matus, José Tomás
Pinto-Carnide, Olinda
Carrasco, David
Arroyo-García, Rosa
Castro, Isaura
author_facet Ferreira, Vanessa
Matus, José Tomás
Pinto-Carnide, Olinda
Carrasco, David
Arroyo-García, Rosa
Castro, Isaura
author_sort Ferreira, Vanessa
collection PubMed
description BACKGROUND: Somatic mutations occurring within meristems of vegetative propagation material have had a major role in increasing the genetic diversity of the domesticated grapevine (Vitis vinifera subsp. vinifera). The most well studied somatic variation in this species is the one affecting fruit pigmentation, leading to a plethora of different berry skin colors. Color depletion and reversion are often observed in the field. In this study we analyzed the origin of a novel white-to-red skin color reversion and studied its possible metabolic and transcriptomic consequences on cv. ‘Muscat à Petits Grains Blancs’ (synonym cv. ‘Moscatel Galego Branco’), a member of the large family of Muscats. RESULTS: The mild red-skinned variant (cv. ‘Muscat à Petits Grains Rouge’, synonym cv. ‘Moscatel Galego Roxo’), characterized by a preferential accumulation of di-hydroxylated anthocyanins, showed in heterozygosis a partially-excised Gret1 retrotransposon in the promoter region of the MYBA1 anthocyanin regulator, while MYBA2 was still in homozygosis for its non-functional allele. Through metabolic (anthocyanin, resveratrol and piceid quantifications) and transcriptomic (RNA-Seq) analyses, we show that within a near-isogenic background, the transcriptomic consequences of color reversion are largely associated to diminished light/UV-B responses probably as a consequence of the augment of metabolic sunscreens (i.e. anthocyanins). CONCLUSIONS: We propose that the reduced activity of the flavonoid tri-hydroxylated sub-branch and decreased anthocyanin synthesis and modification (e.g. methylation and acylation) are the potential causes for the mild red-skinned coloration in the pigmented revertant. The observed positive relation between anthocyanins and stilbenes could be attributable to an increased influx of phenylpropanoid intermediaries due to the replenished activity of MYBA1, an effect yet to be demonstrated in other somatic variants.
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spelling pubmed-69026042019-12-11 Genetic analysis of a white-to-red berry skin color reversion and its transcriptomic and metabolic consequences in grapevine (Vitis vinifera cv. ‘Moscatel Galego’) Ferreira, Vanessa Matus, José Tomás Pinto-Carnide, Olinda Carrasco, David Arroyo-García, Rosa Castro, Isaura BMC Genomics Research Article BACKGROUND: Somatic mutations occurring within meristems of vegetative propagation material have had a major role in increasing the genetic diversity of the domesticated grapevine (Vitis vinifera subsp. vinifera). The most well studied somatic variation in this species is the one affecting fruit pigmentation, leading to a plethora of different berry skin colors. Color depletion and reversion are often observed in the field. In this study we analyzed the origin of a novel white-to-red skin color reversion and studied its possible metabolic and transcriptomic consequences on cv. ‘Muscat à Petits Grains Blancs’ (synonym cv. ‘Moscatel Galego Branco’), a member of the large family of Muscats. RESULTS: The mild red-skinned variant (cv. ‘Muscat à Petits Grains Rouge’, synonym cv. ‘Moscatel Galego Roxo’), characterized by a preferential accumulation of di-hydroxylated anthocyanins, showed in heterozygosis a partially-excised Gret1 retrotransposon in the promoter region of the MYBA1 anthocyanin regulator, while MYBA2 was still in homozygosis for its non-functional allele. Through metabolic (anthocyanin, resveratrol and piceid quantifications) and transcriptomic (RNA-Seq) analyses, we show that within a near-isogenic background, the transcriptomic consequences of color reversion are largely associated to diminished light/UV-B responses probably as a consequence of the augment of metabolic sunscreens (i.e. anthocyanins). CONCLUSIONS: We propose that the reduced activity of the flavonoid tri-hydroxylated sub-branch and decreased anthocyanin synthesis and modification (e.g. methylation and acylation) are the potential causes for the mild red-skinned coloration in the pigmented revertant. The observed positive relation between anthocyanins and stilbenes could be attributable to an increased influx of phenylpropanoid intermediaries due to the replenished activity of MYBA1, an effect yet to be demonstrated in other somatic variants. BioMed Central 2019-12-09 /pmc/articles/PMC6902604/ /pubmed/31815637 http://dx.doi.org/10.1186/s12864-019-6237-5 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Ferreira, Vanessa
Matus, José Tomás
Pinto-Carnide, Olinda
Carrasco, David
Arroyo-García, Rosa
Castro, Isaura
Genetic analysis of a white-to-red berry skin color reversion and its transcriptomic and metabolic consequences in grapevine (Vitis vinifera cv. ‘Moscatel Galego’)
title Genetic analysis of a white-to-red berry skin color reversion and its transcriptomic and metabolic consequences in grapevine (Vitis vinifera cv. ‘Moscatel Galego’)
title_full Genetic analysis of a white-to-red berry skin color reversion and its transcriptomic and metabolic consequences in grapevine (Vitis vinifera cv. ‘Moscatel Galego’)
title_fullStr Genetic analysis of a white-to-red berry skin color reversion and its transcriptomic and metabolic consequences in grapevine (Vitis vinifera cv. ‘Moscatel Galego’)
title_full_unstemmed Genetic analysis of a white-to-red berry skin color reversion and its transcriptomic and metabolic consequences in grapevine (Vitis vinifera cv. ‘Moscatel Galego’)
title_short Genetic analysis of a white-to-red berry skin color reversion and its transcriptomic and metabolic consequences in grapevine (Vitis vinifera cv. ‘Moscatel Galego’)
title_sort genetic analysis of a white-to-red berry skin color reversion and its transcriptomic and metabolic consequences in grapevine (vitis vinifera cv. ‘moscatel galego’)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6902604/
https://www.ncbi.nlm.nih.gov/pubmed/31815637
http://dx.doi.org/10.1186/s12864-019-6237-5
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