A Murine Monoclonal Antibody With Potent Neutralization Ability Against Human Adenovirus 7

B1-type human adenoviruses (HAdVs) HAdV-3, HAdV-7, and HAdV-55 have caused epidemics in North America, Asia, and Europe. However, to date, no adenovirus vaccines or antiviral drugs have been approved for general use. In the present work, a scFv-phage immune library was constructed and mouse monoclon...

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Autores principales: Wang, Rong, Lu, Jiansheng, Zhou, Quan, Chen, Lei, Huang, Ying, Yu, Yunzhou, Yang, Zhixin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Cellular and Infection Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904267/
https://www.ncbi.nlm.nih.gov/pubmed/31867291
http://dx.doi.org/10.3389/fcimb.2019.00417
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author Wang, Rong
Lu, Jiansheng
Zhou, Quan
Chen, Lei
Huang, Ying
Yu, Yunzhou
Yang, Zhixin
author_facet Wang, Rong
Lu, Jiansheng
Zhou, Quan
Chen, Lei
Huang, Ying
Yu, Yunzhou
Yang, Zhixin
author_sort Wang, Rong
collection PubMed
description B1-type human adenoviruses (HAdVs) HAdV-3, HAdV-7, and HAdV-55 have caused epidemics in North America, Asia, and Europe. However, to date, no adenovirus vaccines or antiviral drugs have been approved for general use. In the present work, a scFv-phage immune library was constructed and mouse monoclonal antibody (MMAb) 10G12 was obtained through selection. 10G12 is specific for HAdV-7 and binds the hexon loop1 and loop2 (LP12), resulting in strong neutralization activity against HAdV-7. Additionally, it is stable in serum and buffer at various pH values. The findings provide insight into adenovirus and antibody responses and may facilitate the design and development of adenovirus vaccines and antiviral drugs.
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spelling pubmed-69042672019-12-20 A Murine Monoclonal Antibody With Potent Neutralization Ability Against Human Adenovirus 7 Wang, Rong Lu, Jiansheng Zhou, Quan Chen, Lei Huang, Ying Yu, Yunzhou Yang, Zhixin Front Cell Infect Microbiol Cellular and Infection Microbiology B1-type human adenoviruses (HAdVs) HAdV-3, HAdV-7, and HAdV-55 have caused epidemics in North America, Asia, and Europe. However, to date, no adenovirus vaccines or antiviral drugs have been approved for general use. In the present work, a scFv-phage immune library was constructed and mouse monoclonal antibody (MMAb) 10G12 was obtained through selection. 10G12 is specific for HAdV-7 and binds the hexon loop1 and loop2 (LP12), resulting in strong neutralization activity against HAdV-7. Additionally, it is stable in serum and buffer at various pH values. The findings provide insight into adenovirus and antibody responses and may facilitate the design and development of adenovirus vaccines and antiviral drugs. Frontiers Media S.A. 2019-12-04 /pmc/articles/PMC6904267/ /pubmed/31867291 http://dx.doi.org/10.3389/fcimb.2019.00417 Text en Copyright © 2019 Wang, Lu, Zhou, Chen, Huang, Yu and Yang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Wang, Rong
Lu, Jiansheng
Zhou, Quan
Chen, Lei
Huang, Ying
Yu, Yunzhou
Yang, Zhixin
A Murine Monoclonal Antibody With Potent Neutralization Ability Against Human Adenovirus 7
title A Murine Monoclonal Antibody With Potent Neutralization Ability Against Human Adenovirus 7
title_full A Murine Monoclonal Antibody With Potent Neutralization Ability Against Human Adenovirus 7
title_fullStr A Murine Monoclonal Antibody With Potent Neutralization Ability Against Human Adenovirus 7
title_full_unstemmed A Murine Monoclonal Antibody With Potent Neutralization Ability Against Human Adenovirus 7
title_short A Murine Monoclonal Antibody With Potent Neutralization Ability Against Human Adenovirus 7
title_sort murine monoclonal antibody with potent neutralization ability against human adenovirus 7
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904267/
https://www.ncbi.nlm.nih.gov/pubmed/31867291
http://dx.doi.org/10.3389/fcimb.2019.00417
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