ESR as a monitoring method of the interactions between TEMPO-functionalized magnetic nanoparticles and yeast cells

Potential application of magnetic nanoparticles as drug carriers in medical treatment requires prior determination of their effects on cells. In this work different spin labels and magnetic nanoparticles functionalized with spin labels as well as their interaction with yeast cells were investigated...

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Autores principales: Krzyminiewski, Ryszard, Dobosz, Bernadeta, Schroeder, Grzegorz, Kurczewska, Joanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904674/
https://www.ncbi.nlm.nih.gov/pubmed/31822759
http://dx.doi.org/10.1038/s41598-019-55335-z
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author Krzyminiewski, Ryszard
Dobosz, Bernadeta
Schroeder, Grzegorz
Kurczewska, Joanna
author_facet Krzyminiewski, Ryszard
Dobosz, Bernadeta
Schroeder, Grzegorz
Kurczewska, Joanna
author_sort Krzyminiewski, Ryszard
collection PubMed
description Potential application of magnetic nanoparticles as drug carriers in medical treatment requires prior determination of their effects on cells. In this work different spin labels and magnetic nanoparticles functionalized with spin labels as well as their interaction with yeast cells were investigated using electron spin resonance (ESR) method. ESR was demonstrated to be a suitable method for monitoring of magnetic core and attached spin labels. Particular emphasis was placed on characterization of endocytosis and redox processes running inside the cell, resulting in recombination of spin labels. Such data could only be obtained at reduced temperature of ESR measurements.
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spelling pubmed-69046742019-12-13 ESR as a monitoring method of the interactions between TEMPO-functionalized magnetic nanoparticles and yeast cells Krzyminiewski, Ryszard Dobosz, Bernadeta Schroeder, Grzegorz Kurczewska, Joanna Sci Rep Article Potential application of magnetic nanoparticles as drug carriers in medical treatment requires prior determination of their effects on cells. In this work different spin labels and magnetic nanoparticles functionalized with spin labels as well as their interaction with yeast cells were investigated using electron spin resonance (ESR) method. ESR was demonstrated to be a suitable method for monitoring of magnetic core and attached spin labels. Particular emphasis was placed on characterization of endocytosis and redox processes running inside the cell, resulting in recombination of spin labels. Such data could only be obtained at reduced temperature of ESR measurements. Nature Publishing Group UK 2019-12-10 /pmc/articles/PMC6904674/ /pubmed/31822759 http://dx.doi.org/10.1038/s41598-019-55335-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Krzyminiewski, Ryszard
Dobosz, Bernadeta
Schroeder, Grzegorz
Kurczewska, Joanna
ESR as a monitoring method of the interactions between TEMPO-functionalized magnetic nanoparticles and yeast cells
title ESR as a monitoring method of the interactions between TEMPO-functionalized magnetic nanoparticles and yeast cells
title_full ESR as a monitoring method of the interactions between TEMPO-functionalized magnetic nanoparticles and yeast cells
title_fullStr ESR as a monitoring method of the interactions between TEMPO-functionalized magnetic nanoparticles and yeast cells
title_full_unstemmed ESR as a monitoring method of the interactions between TEMPO-functionalized magnetic nanoparticles and yeast cells
title_short ESR as a monitoring method of the interactions between TEMPO-functionalized magnetic nanoparticles and yeast cells
title_sort esr as a monitoring method of the interactions between tempo-functionalized magnetic nanoparticles and yeast cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904674/
https://www.ncbi.nlm.nih.gov/pubmed/31822759
http://dx.doi.org/10.1038/s41598-019-55335-z
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