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Fission of Lipid-Vesicles by Membrane Phase Transitions in Thermal Convection

Unilamellar lipid vesicles can serve as model for protocells. We present a vesicle fission mechanism in a thermal gradient under flow in a convection chamber, where vesicles cycle cold and hot regions periodically. Crucial to obtain fission of the vesicles in this scenario is a temperature-induced m...

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Autores principales: Kudella, Patrick W., Preißinger, Katharina, Morasch, Matthias, Dirscherl, Christina F., Braun, Dieter, Wixforth, Achim, Westerhausen, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906453/
https://www.ncbi.nlm.nih.gov/pubmed/31827164
http://dx.doi.org/10.1038/s41598-019-55110-0
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author Kudella, Patrick W.
Preißinger, Katharina
Morasch, Matthias
Dirscherl, Christina F.
Braun, Dieter
Wixforth, Achim
Westerhausen, Christoph
author_facet Kudella, Patrick W.
Preißinger, Katharina
Morasch, Matthias
Dirscherl, Christina F.
Braun, Dieter
Wixforth, Achim
Westerhausen, Christoph
author_sort Kudella, Patrick W.
collection PubMed
description Unilamellar lipid vesicles can serve as model for protocells. We present a vesicle fission mechanism in a thermal gradient under flow in a convection chamber, where vesicles cycle cold and hot regions periodically. Crucial to obtain fission of the vesicles in this scenario is a temperature-induced membrane phase transition that vesicles experience multiple times. We model the temperature gradient of the chamber with a capillary to study single vesicles on their way through the temperature gradient in an external field of shear forces. Starting in the gel-like phase the spherical vesicles are heated above their main melting temperature resulting in a dumbbell-deformation. Further downstream a temperature drop below the transition temperature induces splitting of the vesicles without further physical or chemical intervention. This mechanism also holds for less cooperative systems, as shown here for a lipid alloy with a broad transition temperature width of 8 K. We find a critical tether length that can be understood from the transition width and the locally applied temperature gradient. This combination of a temperature-induced membrane phase transition and realistic flow scenarios as given e.g. in a white smoker enable a fission mechanism that can contribute to the understanding of more advanced protocell cycles.
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spelling pubmed-69064532019-12-13 Fission of Lipid-Vesicles by Membrane Phase Transitions in Thermal Convection Kudella, Patrick W. Preißinger, Katharina Morasch, Matthias Dirscherl, Christina F. Braun, Dieter Wixforth, Achim Westerhausen, Christoph Sci Rep Article Unilamellar lipid vesicles can serve as model for protocells. We present a vesicle fission mechanism in a thermal gradient under flow in a convection chamber, where vesicles cycle cold and hot regions periodically. Crucial to obtain fission of the vesicles in this scenario is a temperature-induced membrane phase transition that vesicles experience multiple times. We model the temperature gradient of the chamber with a capillary to study single vesicles on their way through the temperature gradient in an external field of shear forces. Starting in the gel-like phase the spherical vesicles are heated above their main melting temperature resulting in a dumbbell-deformation. Further downstream a temperature drop below the transition temperature induces splitting of the vesicles without further physical or chemical intervention. This mechanism also holds for less cooperative systems, as shown here for a lipid alloy with a broad transition temperature width of 8 K. We find a critical tether length that can be understood from the transition width and the locally applied temperature gradient. This combination of a temperature-induced membrane phase transition and realistic flow scenarios as given e.g. in a white smoker enable a fission mechanism that can contribute to the understanding of more advanced protocell cycles. Nature Publishing Group UK 2019-12-11 /pmc/articles/PMC6906453/ /pubmed/31827164 http://dx.doi.org/10.1038/s41598-019-55110-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kudella, Patrick W.
Preißinger, Katharina
Morasch, Matthias
Dirscherl, Christina F.
Braun, Dieter
Wixforth, Achim
Westerhausen, Christoph
Fission of Lipid-Vesicles by Membrane Phase Transitions in Thermal Convection
title Fission of Lipid-Vesicles by Membrane Phase Transitions in Thermal Convection
title_full Fission of Lipid-Vesicles by Membrane Phase Transitions in Thermal Convection
title_fullStr Fission of Lipid-Vesicles by Membrane Phase Transitions in Thermal Convection
title_full_unstemmed Fission of Lipid-Vesicles by Membrane Phase Transitions in Thermal Convection
title_short Fission of Lipid-Vesicles by Membrane Phase Transitions in Thermal Convection
title_sort fission of lipid-vesicles by membrane phase transitions in thermal convection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906453/
https://www.ncbi.nlm.nih.gov/pubmed/31827164
http://dx.doi.org/10.1038/s41598-019-55110-0
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