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Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation

Infection is the leading cause of non-relapse-related mortality after allogeneic haematopoietic stem cell transplantation (HSCT). Altered functions of immune cells in nasal secretions may influence post HSCT susceptibility to viral respiratory infections. In this prospective study, we determined T a...

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Autores principales: Vela, Maria, del Rosal, Teresa, Pérez-Martínez, Antonio, Valentín, Jaime, Casas, Inmaculada, Pozo, Francisco, Reinoso-Barbero, Francisco, Bueno, David, Corral, Dolores, Méndez-Echevarría, Ana, Mozo, Yasmina, Calvo, Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906525/
https://www.ncbi.nlm.nih.gov/pubmed/31827202
http://dx.doi.org/10.1038/s41598-019-55398-y
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author Vela, Maria
del Rosal, Teresa
Pérez-Martínez, Antonio
Valentín, Jaime
Casas, Inmaculada
Pozo, Francisco
Reinoso-Barbero, Francisco
Bueno, David
Corral, Dolores
Méndez-Echevarría, Ana
Mozo, Yasmina
Calvo, Cristina
author_facet Vela, Maria
del Rosal, Teresa
Pérez-Martínez, Antonio
Valentín, Jaime
Casas, Inmaculada
Pozo, Francisco
Reinoso-Barbero, Francisco
Bueno, David
Corral, Dolores
Méndez-Echevarría, Ana
Mozo, Yasmina
Calvo, Cristina
author_sort Vela, Maria
collection PubMed
description Infection is the leading cause of non-relapse-related mortality after allogeneic haematopoietic stem cell transplantation (HSCT). Altered functions of immune cells in nasal secretions may influence post HSCT susceptibility to viral respiratory infections. In this prospective study, we determined T and NK cell numbers together with NK activation status in nasopharyngeal aspirates (NPA) in HSCT recipients and healthy controls using multiparametric flow cytometry. We also determined by polymerase chain reaction (PCR) the presence of 16 respiratory viruses. Samples were collected pre-HSCT, at day 0, +10, +20 and +30 after HSCT. Peripheral blood (PB) was also analyzed to determine T and NK cell numbers. A total of 27 pediatric HSCT recipients were enrolled and 16 of them had at least one viral detection (60%). Rhinovirus was the most frequent pathogen (84% of positive NPAs). NPAs of patients contained fewer T and NK cells compared to healthy controls (p = 0.0132 and p = 0.120, respectively). Viral PCR + patients showed higher NK cell number in their NPAs. The activating receptors repertoire expressed by NK cells was also higher in NPA samples, especially NKp44 and NKp46. Our study supports NK cells relevance for the immune defense against respiratory viruses in HSCT recipients.
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spelling pubmed-69065252019-12-13 Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation Vela, Maria del Rosal, Teresa Pérez-Martínez, Antonio Valentín, Jaime Casas, Inmaculada Pozo, Francisco Reinoso-Barbero, Francisco Bueno, David Corral, Dolores Méndez-Echevarría, Ana Mozo, Yasmina Calvo, Cristina Sci Rep Article Infection is the leading cause of non-relapse-related mortality after allogeneic haematopoietic stem cell transplantation (HSCT). Altered functions of immune cells in nasal secretions may influence post HSCT susceptibility to viral respiratory infections. In this prospective study, we determined T and NK cell numbers together with NK activation status in nasopharyngeal aspirates (NPA) in HSCT recipients and healthy controls using multiparametric flow cytometry. We also determined by polymerase chain reaction (PCR) the presence of 16 respiratory viruses. Samples were collected pre-HSCT, at day 0, +10, +20 and +30 after HSCT. Peripheral blood (PB) was also analyzed to determine T and NK cell numbers. A total of 27 pediatric HSCT recipients were enrolled and 16 of them had at least one viral detection (60%). Rhinovirus was the most frequent pathogen (84% of positive NPAs). NPAs of patients contained fewer T and NK cells compared to healthy controls (p = 0.0132 and p = 0.120, respectively). Viral PCR + patients showed higher NK cell number in their NPAs. The activating receptors repertoire expressed by NK cells was also higher in NPA samples, especially NKp44 and NKp46. Our study supports NK cells relevance for the immune defense against respiratory viruses in HSCT recipients. Nature Publishing Group UK 2019-12-11 /pmc/articles/PMC6906525/ /pubmed/31827202 http://dx.doi.org/10.1038/s41598-019-55398-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Vela, Maria
del Rosal, Teresa
Pérez-Martínez, Antonio
Valentín, Jaime
Casas, Inmaculada
Pozo, Francisco
Reinoso-Barbero, Francisco
Bueno, David
Corral, Dolores
Méndez-Echevarría, Ana
Mozo, Yasmina
Calvo, Cristina
Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation
title Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation
title_full Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation
title_fullStr Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation
title_full_unstemmed Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation
title_short Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation
title_sort possible role of highly activated mucosal nk cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906525/
https://www.ncbi.nlm.nih.gov/pubmed/31827202
http://dx.doi.org/10.1038/s41598-019-55398-y
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