3D-printed biological cell phantom for testing 3D quantitative phase imaging systems

As the 3D quantitative phase imaging (QPI) methods mature, their further development calls for reliable tools and methods to characterize and compare their metrological parameters. We use refractive index engineering during two-photon laser photolithography to fabricate a life-scale phantom of a bio...

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Detalles Bibliográficos
Autores principales: Ziemczonok, Michał, Kuś, Arkadiusz, Wasylczyk, Piotr, Kujawińska, Małgorzata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906528/
https://www.ncbi.nlm.nih.gov/pubmed/31827171
http://dx.doi.org/10.1038/s41598-019-55330-4
Descripción
Sumario:As the 3D quantitative phase imaging (QPI) methods mature, their further development calls for reliable tools and methods to characterize and compare their metrological parameters. We use refractive index engineering during two-photon laser photolithography to fabricate a life-scale phantom of a biological cell with internal structures that mimic optical and structural properties of mammalian cells. After verification with a number of reference techniques, the phantom is used to characterize the performance of a limited-angle holographic tomography microscope.

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