3D-printed biological cell phantom for testing 3D quantitative phase imaging systems

As the 3D quantitative phase imaging (QPI) methods mature, their further development calls for reliable tools and methods to characterize and compare their metrological parameters. We use refractive index engineering during two-photon laser photolithography to fabricate a life-scale phantom of a bio...

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Autores principales: Ziemczonok, Michał, Kuś, Arkadiusz, Wasylczyk, Piotr, Kujawińska, Małgorzata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906528/
https://www.ncbi.nlm.nih.gov/pubmed/31827171
http://dx.doi.org/10.1038/s41598-019-55330-4
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author Ziemczonok, Michał
Kuś, Arkadiusz
Wasylczyk, Piotr
Kujawińska, Małgorzata
author_facet Ziemczonok, Michał
Kuś, Arkadiusz
Wasylczyk, Piotr
Kujawińska, Małgorzata
author_sort Ziemczonok, Michał
collection PubMed
description As the 3D quantitative phase imaging (QPI) methods mature, their further development calls for reliable tools and methods to characterize and compare their metrological parameters. We use refractive index engineering during two-photon laser photolithography to fabricate a life-scale phantom of a biological cell with internal structures that mimic optical and structural properties of mammalian cells. After verification with a number of reference techniques, the phantom is used to characterize the performance of a limited-angle holographic tomography microscope.
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spelling pubmed-69065282019-12-13 3D-printed biological cell phantom for testing 3D quantitative phase imaging systems Ziemczonok, Michał Kuś, Arkadiusz Wasylczyk, Piotr Kujawińska, Małgorzata Sci Rep Article As the 3D quantitative phase imaging (QPI) methods mature, their further development calls for reliable tools and methods to characterize and compare their metrological parameters. We use refractive index engineering during two-photon laser photolithography to fabricate a life-scale phantom of a biological cell with internal structures that mimic optical and structural properties of mammalian cells. After verification with a number of reference techniques, the phantom is used to characterize the performance of a limited-angle holographic tomography microscope. Nature Publishing Group UK 2019-12-11 /pmc/articles/PMC6906528/ /pubmed/31827171 http://dx.doi.org/10.1038/s41598-019-55330-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ziemczonok, Michał
Kuś, Arkadiusz
Wasylczyk, Piotr
Kujawińska, Małgorzata
3D-printed biological cell phantom for testing 3D quantitative phase imaging systems
title 3D-printed biological cell phantom for testing 3D quantitative phase imaging systems
title_full 3D-printed biological cell phantom for testing 3D quantitative phase imaging systems
title_fullStr 3D-printed biological cell phantom for testing 3D quantitative phase imaging systems
title_full_unstemmed 3D-printed biological cell phantom for testing 3D quantitative phase imaging systems
title_short 3D-printed biological cell phantom for testing 3D quantitative phase imaging systems
title_sort 3d-printed biological cell phantom for testing 3d quantitative phase imaging systems
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906528/
https://www.ncbi.nlm.nih.gov/pubmed/31827171
http://dx.doi.org/10.1038/s41598-019-55330-4
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