CRISPR-Cas12a/Cpf1-assisted precise, efficient and multiplexed genome-editing in Yarrowia lipolytica

CRISPR-Cas9 has been widely adopted as the basic toolkit for precise genome-editing and engineering in various organisms. Alternative to Cas9, Cas12 or Cpf1 uses a simple crRNA as a guide and expands the protospacer adjacent motif (PAM) sequence to TTTN. This unique PAM sequence of Cpf1 may signific...

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Detalles Bibliográficos
Autores principales: Yang, Zhiliang, Edwards, Harley, Xu, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906711/
https://www.ncbi.nlm.nih.gov/pubmed/31867213
http://dx.doi.org/10.1016/j.mec.2019.e00112

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906711/
https://www.ncbi.nlm.nih.gov/pubmed/31867213
http://dx.doi.org/10.1016/j.mec.2019.e00112

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