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Thermostable β-Lactamase Mutant with Its Active Site Conjugated with Fluorescein for Efficient β-Lactam Antibiotic Detection

[Image: see text] Monitoring the β-lactam antibiotic level has been an important task in food industry and clinical practice. Here, we report the development of a fluorescent PenP β-lactamase, PenP-E166Cf/N170Q, for efficient β-lactam antibiotic detection. It was constructed by covalently attaching...

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Detalles Bibliográficos
Autores principales:	Au, Ho-Wah, Tsang, Man-Wah, So, Pui-Kin, Wong, Kwok-Yin, Leung, Yun-Chung
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2019
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6906784/ https://www.ncbi.nlm.nih.gov/pubmed/31858033 http://dx.doi.org/10.1021/acsomega.9b02211

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