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Simultaneous Assay of Dexchlorpheniramine Maleate, Betamethasone, and Sodium Benzoate in Syrup by a Reliable and Robust HPLC Method

The simultaneous determination of betamethasone, dexchlorpheniramine maleate, and sodium benzoate in pharmaceutical syrup was done by using a simple validated HPLC method. The chromatographic separation of the three analytes was done in a C18 column maintained at 25°C, using a mixture of acetonitril...

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Detalles Bibliográficos
Autores principales: Le, Dinh Chi, Ngo, Thi Duyen, Hoa Le, Thi Huong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6907040/
https://www.ncbi.nlm.nih.gov/pubmed/31871819
http://dx.doi.org/10.1155/2019/2952075
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author Le, Dinh Chi
Ngo, Thi Duyen
Hoa Le, Thi Huong
author_facet Le, Dinh Chi
Ngo, Thi Duyen
Hoa Le, Thi Huong
author_sort Le, Dinh Chi
collection PubMed
description The simultaneous determination of betamethasone, dexchlorpheniramine maleate, and sodium benzoate in pharmaceutical syrup was done by using a simple validated HPLC method. The chromatographic separation of the three analytes was done in a C18 column maintained at 25°C, using a mixture of acetonitrile and 0.02 M phosphate buffer solution pH 2.70 (35 : 65, v : v) as mobile phase. The isocratic elution was chosen with total flow rate of mobile phase maintained at 1.0 mL per minute. The analytes were detected by a UV-Vis detector set at 254 nm. Injection volume was set at 50 μl. The method was fully validated in terms of specificity, linearity, precision, accuracy, and robustness according to requirements of current guidelines and was proven to be suitable for the intended application.
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spelling pubmed-69070402019-12-23 Simultaneous Assay of Dexchlorpheniramine Maleate, Betamethasone, and Sodium Benzoate in Syrup by a Reliable and Robust HPLC Method Le, Dinh Chi Ngo, Thi Duyen Hoa Le, Thi Huong J Anal Methods Chem Research Article The simultaneous determination of betamethasone, dexchlorpheniramine maleate, and sodium benzoate in pharmaceutical syrup was done by using a simple validated HPLC method. The chromatographic separation of the three analytes was done in a C18 column maintained at 25°C, using a mixture of acetonitrile and 0.02 M phosphate buffer solution pH 2.70 (35 : 65, v : v) as mobile phase. The isocratic elution was chosen with total flow rate of mobile phase maintained at 1.0 mL per minute. The analytes were detected by a UV-Vis detector set at 254 nm. Injection volume was set at 50 μl. The method was fully validated in terms of specificity, linearity, precision, accuracy, and robustness according to requirements of current guidelines and was proven to be suitable for the intended application. Hindawi 2019-11-29 /pmc/articles/PMC6907040/ /pubmed/31871819 http://dx.doi.org/10.1155/2019/2952075 Text en Copyright © 2019 Dinh Chi Le et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Le, Dinh Chi
Ngo, Thi Duyen
Hoa Le, Thi Huong
Simultaneous Assay of Dexchlorpheniramine Maleate, Betamethasone, and Sodium Benzoate in Syrup by a Reliable and Robust HPLC Method
title Simultaneous Assay of Dexchlorpheniramine Maleate, Betamethasone, and Sodium Benzoate in Syrup by a Reliable and Robust HPLC Method
title_full Simultaneous Assay of Dexchlorpheniramine Maleate, Betamethasone, and Sodium Benzoate in Syrup by a Reliable and Robust HPLC Method
title_fullStr Simultaneous Assay of Dexchlorpheniramine Maleate, Betamethasone, and Sodium Benzoate in Syrup by a Reliable and Robust HPLC Method
title_full_unstemmed Simultaneous Assay of Dexchlorpheniramine Maleate, Betamethasone, and Sodium Benzoate in Syrup by a Reliable and Robust HPLC Method
title_short Simultaneous Assay of Dexchlorpheniramine Maleate, Betamethasone, and Sodium Benzoate in Syrup by a Reliable and Robust HPLC Method
title_sort simultaneous assay of dexchlorpheniramine maleate, betamethasone, and sodium benzoate in syrup by a reliable and robust hplc method
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6907040/
https://www.ncbi.nlm.nih.gov/pubmed/31871819
http://dx.doi.org/10.1155/2019/2952075
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