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NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression
PURPOSE: Osteosarcoma is one of the most common primary malignant, aggressive bone neoplasms. However, the mechanisms of osteosarcoma proliferation, migration, and invasion are not well understood. To explore the possible mechanism of osteosarcoma progression, we used a public database for gene anal...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6907048/ https://www.ncbi.nlm.nih.gov/pubmed/31886210 http://dx.doi.org/10.1155/2019/4068521 |
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author | Xu, Jiaxing Xu, Gongping Zhang, Tingxin Chen, Tiantian Zhao, Wei Wang, Guangyou |
author_facet | Xu, Jiaxing Xu, Gongping Zhang, Tingxin Chen, Tiantian Zhao, Wei Wang, Guangyou |
author_sort | Xu, Jiaxing |
collection | PubMed |
description | PURPOSE: Osteosarcoma is one of the most common primary malignant, aggressive bone neoplasms. However, the mechanisms of osteosarcoma proliferation, migration, and invasion are not well understood. To explore the possible mechanism of osteosarcoma progression, we used a public database for gene analysis to identify the possible factors that are important in osteosarcoma progression. Nuclear factor interleukin 3 (NFIL3) regulated was highly expressed in sarcoma tissues. In this study, we meant to probe the function of NFIL3 in osteosarcoma proliferation, migration, and invasion. METHODS: The expression of NFIL3 in osteosarcoma tissues was analysed via RT-PCR and immunohistochemistry staining. In order to elucidate the function of NFIL3 in osteosarcoma, we performed cell growth assays and colony formation assays to explore the role of NFIL3 in proliferation in osteosarcoma cells. Futhermore, we analysed osteosarcoma cell migration and invasion via wound healing assays and transwell migration and invasion assays. RESULTS: NFIL3 is overexpressed in osteosarcoma tissues; 15 of the 20 osteosarcoma tissues analysed highly expressed NFIL3. Our in vitro experiments confirmed that NFIL3 promoted the proliferation of M6-63 and SaOS2 cells (P < 0.01). In addition, NFIL3 promoted the migration and invasion of osteosarcoma cells (P < 0.05). CONCLUSION: NFIL3 is highly expressed in osteosarcoma tissues and thus promotes the proliferation, migration, and invasion of osteosarcoma cells. NFIL3 is potential to become a new target for development of novel treatment strategies of osteosarcoma. |
format | Online Article Text |
id | pubmed-6907048 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-69070482019-12-29 NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression Xu, Jiaxing Xu, Gongping Zhang, Tingxin Chen, Tiantian Zhao, Wei Wang, Guangyou Biomed Res Int Research Article PURPOSE: Osteosarcoma is one of the most common primary malignant, aggressive bone neoplasms. However, the mechanisms of osteosarcoma proliferation, migration, and invasion are not well understood. To explore the possible mechanism of osteosarcoma progression, we used a public database for gene analysis to identify the possible factors that are important in osteosarcoma progression. Nuclear factor interleukin 3 (NFIL3) regulated was highly expressed in sarcoma tissues. In this study, we meant to probe the function of NFIL3 in osteosarcoma proliferation, migration, and invasion. METHODS: The expression of NFIL3 in osteosarcoma tissues was analysed via RT-PCR and immunohistochemistry staining. In order to elucidate the function of NFIL3 in osteosarcoma, we performed cell growth assays and colony formation assays to explore the role of NFIL3 in proliferation in osteosarcoma cells. Futhermore, we analysed osteosarcoma cell migration and invasion via wound healing assays and transwell migration and invasion assays. RESULTS: NFIL3 is overexpressed in osteosarcoma tissues; 15 of the 20 osteosarcoma tissues analysed highly expressed NFIL3. Our in vitro experiments confirmed that NFIL3 promoted the proliferation of M6-63 and SaOS2 cells (P < 0.01). In addition, NFIL3 promoted the migration and invasion of osteosarcoma cells (P < 0.05). CONCLUSION: NFIL3 is highly expressed in osteosarcoma tissues and thus promotes the proliferation, migration, and invasion of osteosarcoma cells. NFIL3 is potential to become a new target for development of novel treatment strategies of osteosarcoma. Hindawi 2019-11-30 /pmc/articles/PMC6907048/ /pubmed/31886210 http://dx.doi.org/10.1155/2019/4068521 Text en Copyright © 2019 Jiaxing Xu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Xu, Jiaxing Xu, Gongping Zhang, Tingxin Chen, Tiantian Zhao, Wei Wang, Guangyou NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression |
title | NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression |
title_full | NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression |
title_fullStr | NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression |
title_full_unstemmed | NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression |
title_short | NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression |
title_sort | nfil3 acts as a nuclear factor to increase osteosarcoma progression |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6907048/ https://www.ncbi.nlm.nih.gov/pubmed/31886210 http://dx.doi.org/10.1155/2019/4068521 |
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