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NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression

PURPOSE: Osteosarcoma is one of the most common primary malignant, aggressive bone neoplasms. However, the mechanisms of osteosarcoma proliferation, migration, and invasion are not well understood. To explore the possible mechanism of osteosarcoma progression, we used a public database for gene anal...

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Autores principales: Xu, Jiaxing, Xu, Gongping, Zhang, Tingxin, Chen, Tiantian, Zhao, Wei, Wang, Guangyou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6907048/
https://www.ncbi.nlm.nih.gov/pubmed/31886210
http://dx.doi.org/10.1155/2019/4068521
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author Xu, Jiaxing
Xu, Gongping
Zhang, Tingxin
Chen, Tiantian
Zhao, Wei
Wang, Guangyou
author_facet Xu, Jiaxing
Xu, Gongping
Zhang, Tingxin
Chen, Tiantian
Zhao, Wei
Wang, Guangyou
author_sort Xu, Jiaxing
collection PubMed
description PURPOSE: Osteosarcoma is one of the most common primary malignant, aggressive bone neoplasms. However, the mechanisms of osteosarcoma proliferation, migration, and invasion are not well understood. To explore the possible mechanism of osteosarcoma progression, we used a public database for gene analysis to identify the possible factors that are important in osteosarcoma progression. Nuclear factor interleukin 3 (NFIL3) regulated was highly expressed in sarcoma tissues. In this study, we meant to probe the function of NFIL3 in osteosarcoma proliferation, migration, and invasion. METHODS: The expression of NFIL3 in osteosarcoma tissues was analysed via RT-PCR and immunohistochemistry staining. In order to elucidate the function of NFIL3 in osteosarcoma, we performed cell growth assays and colony formation assays to explore the role of NFIL3 in proliferation in osteosarcoma cells. Futhermore, we analysed osteosarcoma cell migration and invasion via wound healing assays and transwell migration and invasion assays. RESULTS: NFIL3 is overexpressed in osteosarcoma tissues; 15 of the 20 osteosarcoma tissues analysed highly expressed NFIL3. Our in vitro experiments confirmed that NFIL3 promoted the proliferation of M6-63 and SaOS2 cells (P < 0.01). In addition, NFIL3 promoted the migration and invasion of osteosarcoma cells (P < 0.05). CONCLUSION: NFIL3 is highly expressed in osteosarcoma tissues and thus promotes the proliferation, migration, and invasion of osteosarcoma cells. NFIL3 is potential to become a new target for development of novel treatment strategies of osteosarcoma.
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spelling pubmed-69070482019-12-29 NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression Xu, Jiaxing Xu, Gongping Zhang, Tingxin Chen, Tiantian Zhao, Wei Wang, Guangyou Biomed Res Int Research Article PURPOSE: Osteosarcoma is one of the most common primary malignant, aggressive bone neoplasms. However, the mechanisms of osteosarcoma proliferation, migration, and invasion are not well understood. To explore the possible mechanism of osteosarcoma progression, we used a public database for gene analysis to identify the possible factors that are important in osteosarcoma progression. Nuclear factor interleukin 3 (NFIL3) regulated was highly expressed in sarcoma tissues. In this study, we meant to probe the function of NFIL3 in osteosarcoma proliferation, migration, and invasion. METHODS: The expression of NFIL3 in osteosarcoma tissues was analysed via RT-PCR and immunohistochemistry staining. In order to elucidate the function of NFIL3 in osteosarcoma, we performed cell growth assays and colony formation assays to explore the role of NFIL3 in proliferation in osteosarcoma cells. Futhermore, we analysed osteosarcoma cell migration and invasion via wound healing assays and transwell migration and invasion assays. RESULTS: NFIL3 is overexpressed in osteosarcoma tissues; 15 of the 20 osteosarcoma tissues analysed highly expressed NFIL3. Our in vitro experiments confirmed that NFIL3 promoted the proliferation of M6-63 and SaOS2 cells (P < 0.01). In addition, NFIL3 promoted the migration and invasion of osteosarcoma cells (P < 0.05). CONCLUSION: NFIL3 is highly expressed in osteosarcoma tissues and thus promotes the proliferation, migration, and invasion of osteosarcoma cells. NFIL3 is potential to become a new target for development of novel treatment strategies of osteosarcoma. Hindawi 2019-11-30 /pmc/articles/PMC6907048/ /pubmed/31886210 http://dx.doi.org/10.1155/2019/4068521 Text en Copyright © 2019 Jiaxing Xu et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xu, Jiaxing
Xu, Gongping
Zhang, Tingxin
Chen, Tiantian
Zhao, Wei
Wang, Guangyou
NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression
title NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression
title_full NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression
title_fullStr NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression
title_full_unstemmed NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression
title_short NFIL3 Acts as a Nuclear Factor to Increase Osteosarcoma Progression
title_sort nfil3 acts as a nuclear factor to increase osteosarcoma progression
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6907048/
https://www.ncbi.nlm.nih.gov/pubmed/31886210
http://dx.doi.org/10.1155/2019/4068521
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