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Synthesis of silver nanoparticles using a Mentha spicata extract and evaluation of its anticancer and cytotoxic activity

In this study, silver nanoparticles (NP) were synthesized by two methods: using an aqueous extract of Mentha spicata leaves and using citrate ions as stabilizing agent, and the cytotoxicity and anticancer activity of both NP were evaluated in vitro. The particles synthesized with the aqueous extract...

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Autores principales: Torres-Martínez, Yuridia, Arredondo-Espinoza, Eder, Puente, Carlos, González-Santiago, Omar, Pineda-Aguilar, Nayely, Balderas-Rentería, Isaías, López, Israel, Ramírez-Cabrera, Mónica A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6907090/
https://www.ncbi.nlm.nih.gov/pubmed/31844570
http://dx.doi.org/10.7717/peerj.8142
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author Torres-Martínez, Yuridia
Arredondo-Espinoza, Eder
Puente, Carlos
González-Santiago, Omar
Pineda-Aguilar, Nayely
Balderas-Rentería, Isaías
López, Israel
Ramírez-Cabrera, Mónica A.
author_facet Torres-Martínez, Yuridia
Arredondo-Espinoza, Eder
Puente, Carlos
González-Santiago, Omar
Pineda-Aguilar, Nayely
Balderas-Rentería, Isaías
López, Israel
Ramírez-Cabrera, Mónica A.
author_sort Torres-Martínez, Yuridia
collection PubMed
description In this study, silver nanoparticles (NP) were synthesized by two methods: using an aqueous extract of Mentha spicata leaves and using citrate ions as stabilizing agent, and the cytotoxicity and anticancer activity of both NP were evaluated in vitro. The particles synthesized with the aqueous extract were spherical with a size ranging from 15 to 45 nm. These NP decreased cell viability in all of the cells studied; however, the IC(50) could only be estimated in the Chang liver cells (IC(50) = 21.37 µg/mL). These particles also decreased the generation of reactive oxygen species in Chang and SiHa cells. Additionally, the dispersions decreased the activity of caspase-3. There was no significant difference between the biological activities of the NP obtained with the aqueous extract and the NP synthesized using citrate ions. This study showed that an aqueous extract of M. spicata is an excellent alternative for the synthesis of silver NP. These NP showed cytotoxicity and anticancer activity in vitro. Although more experiments are required, the cell death occurs probably through a mechanism different from apoptosis.
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spelling pubmed-69070902019-12-16 Synthesis of silver nanoparticles using a Mentha spicata extract and evaluation of its anticancer and cytotoxic activity Torres-Martínez, Yuridia Arredondo-Espinoza, Eder Puente, Carlos González-Santiago, Omar Pineda-Aguilar, Nayely Balderas-Rentería, Isaías López, Israel Ramírez-Cabrera, Mónica A. PeerJ Cell Biology In this study, silver nanoparticles (NP) were synthesized by two methods: using an aqueous extract of Mentha spicata leaves and using citrate ions as stabilizing agent, and the cytotoxicity and anticancer activity of both NP were evaluated in vitro. The particles synthesized with the aqueous extract were spherical with a size ranging from 15 to 45 nm. These NP decreased cell viability in all of the cells studied; however, the IC(50) could only be estimated in the Chang liver cells (IC(50) = 21.37 µg/mL). These particles also decreased the generation of reactive oxygen species in Chang and SiHa cells. Additionally, the dispersions decreased the activity of caspase-3. There was no significant difference between the biological activities of the NP obtained with the aqueous extract and the NP synthesized using citrate ions. This study showed that an aqueous extract of M. spicata is an excellent alternative for the synthesis of silver NP. These NP showed cytotoxicity and anticancer activity in vitro. Although more experiments are required, the cell death occurs probably through a mechanism different from apoptosis. PeerJ Inc. 2019-12-09 /pmc/articles/PMC6907090/ /pubmed/31844570 http://dx.doi.org/10.7717/peerj.8142 Text en © 2019 Torres-Martínez et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Cell Biology
Torres-Martínez, Yuridia
Arredondo-Espinoza, Eder
Puente, Carlos
González-Santiago, Omar
Pineda-Aguilar, Nayely
Balderas-Rentería, Isaías
López, Israel
Ramírez-Cabrera, Mónica A.
Synthesis of silver nanoparticles using a Mentha spicata extract and evaluation of its anticancer and cytotoxic activity
title Synthesis of silver nanoparticles using a Mentha spicata extract and evaluation of its anticancer and cytotoxic activity
title_full Synthesis of silver nanoparticles using a Mentha spicata extract and evaluation of its anticancer and cytotoxic activity
title_fullStr Synthesis of silver nanoparticles using a Mentha spicata extract and evaluation of its anticancer and cytotoxic activity
title_full_unstemmed Synthesis of silver nanoparticles using a Mentha spicata extract and evaluation of its anticancer and cytotoxic activity
title_short Synthesis of silver nanoparticles using a Mentha spicata extract and evaluation of its anticancer and cytotoxic activity
title_sort synthesis of silver nanoparticles using a mentha spicata extract and evaluation of its anticancer and cytotoxic activity
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6907090/
https://www.ncbi.nlm.nih.gov/pubmed/31844570
http://dx.doi.org/10.7717/peerj.8142
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