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Optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems

The excited state lifetime of a fluorophore together with its fluorescence emission spectrum provide information that can yield valuable insights into the nature of a fluorophore and its microenvironment. However, it is difficult to obtain both channels of information in a conventional scheme as det...

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Autores principales: Sagar, Md Abdul Kader, Dai, Bing, Chacko, Jenu V., Weber, Joshua J., Velten, Andreas, Sanders, Scott T., White, John G., Eliceiri, Kevin W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Photo-Optical Instrumentation Engineers 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6907392/
https://www.ncbi.nlm.nih.gov/pubmed/31833280
http://dx.doi.org/10.1117/1.JBO.25.1.014506
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author Sagar, Md Abdul Kader
Dai, Bing
Chacko, Jenu V.
Weber, Joshua J.
Velten, Andreas
Sanders, Scott T.
White, John G.
Eliceiri, Kevin W.
author_facet Sagar, Md Abdul Kader
Dai, Bing
Chacko, Jenu V.
Weber, Joshua J.
Velten, Andreas
Sanders, Scott T.
White, John G.
Eliceiri, Kevin W.
author_sort Sagar, Md Abdul Kader
collection PubMed
description The excited state lifetime of a fluorophore together with its fluorescence emission spectrum provide information that can yield valuable insights into the nature of a fluorophore and its microenvironment. However, it is difficult to obtain both channels of information in a conventional scheme as detectors are typically configured either for spectral or lifetime detection. We present a fiber-based method to obtain spectral information from a multiphoton fluorescence lifetime imaging (FLIM) system. This is made possible using the time delay introduced in the fluorescence emission path by a dispersive optical fiber coupled to a detector operating in time-correlated single-photon counting mode. This add-on spectral implementation requires only a few simple modifications to any existing FLIM system and is considerably more cost-efficient compared to currently available spectral detectors.
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spelling pubmed-69073922020-02-10 Optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems Sagar, Md Abdul Kader Dai, Bing Chacko, Jenu V. Weber, Joshua J. Velten, Andreas Sanders, Scott T. White, John G. Eliceiri, Kevin W. J Biomed Opt Special Section Celebrating Thirty Years of Multiphoton Microscopy in the Biomedical Sciences The excited state lifetime of a fluorophore together with its fluorescence emission spectrum provide information that can yield valuable insights into the nature of a fluorophore and its microenvironment. However, it is difficult to obtain both channels of information in a conventional scheme as detectors are typically configured either for spectral or lifetime detection. We present a fiber-based method to obtain spectral information from a multiphoton fluorescence lifetime imaging (FLIM) system. This is made possible using the time delay introduced in the fluorescence emission path by a dispersive optical fiber coupled to a detector operating in time-correlated single-photon counting mode. This add-on spectral implementation requires only a few simple modifications to any existing FLIM system and is considerably more cost-efficient compared to currently available spectral detectors. Society of Photo-Optical Instrumentation Engineers 2019-12-12 2020-01 /pmc/articles/PMC6907392/ /pubmed/31833280 http://dx.doi.org/10.1117/1.JBO.25.1.014506 Text en © 2020 The Authors https://creativecommons.org/licenses/by/4.0/ Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
spellingShingle Special Section Celebrating Thirty Years of Multiphoton Microscopy in the Biomedical Sciences
Sagar, Md Abdul Kader
Dai, Bing
Chacko, Jenu V.
Weber, Joshua J.
Velten, Andreas
Sanders, Scott T.
White, John G.
Eliceiri, Kevin W.
Optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems
title Optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems
title_full Optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems
title_fullStr Optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems
title_full_unstemmed Optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems
title_short Optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems
title_sort optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems
topic Special Section Celebrating Thirty Years of Multiphoton Microscopy in the Biomedical Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6907392/
https://www.ncbi.nlm.nih.gov/pubmed/31833280
http://dx.doi.org/10.1117/1.JBO.25.1.014506
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