Extracellular vesicles isolated from Trypanosoma cruzi affect early parasite migration in the gut of Rhodnius prolixus but not in Triatoma infestans

The protozoan Trypanosoma cruzi has the ability to spontaneously secrete extracellular vesicles (EVs). In this paper, T. cruzi EVs derived from epimastigote forms were evaluated during interaction with triatomine bugs Rhodnius prolixus and Triatoma infestans. T. cruzi EVs were purified and artificia...

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Autores principales: Paranaiba, Larissa F, Guarneri, Alessandra A, Torrecilhas, Ana C, Melo, Maria N, Soares, Rodrigo P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2019
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6908325/
https://www.ncbi.nlm.nih.gov/pubmed/31851215
http://dx.doi.org/10.1590/0074-02760190217
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author Paranaiba, Larissa F
Guarneri, Alessandra A
Torrecilhas, Ana C
Melo, Maria N
Soares, Rodrigo P
author_facet Paranaiba, Larissa F
Guarneri, Alessandra A
Torrecilhas, Ana C
Melo, Maria N
Soares, Rodrigo P
author_sort Paranaiba, Larissa F
collection PubMed
description The protozoan Trypanosoma cruzi has the ability to spontaneously secrete extracellular vesicles (EVs). In this paper, T. cruzi EVs derived from epimastigote forms were evaluated during interaction with triatomine bugs Rhodnius prolixus and Triatoma infestans. T. cruzi EVs were purified and artificially offered to the insects prior to infection with epimastigote forms. No effect of EVs was detected in the parasite counts in the guts of both vectors after 49-50 days. On the other hand, pre-feeding with EVs delayed parasite migration to rectum only in the gut in R. prolixus after 21-22 days. Those data suggest a possible role of T. cruzi EVs during the earlier events of infection in the invertebrate host.
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spelling pubmed-69083252019-12-16 Extracellular vesicles isolated from Trypanosoma cruzi affect early parasite migration in the gut of Rhodnius prolixus but not in Triatoma infestans Paranaiba, Larissa F Guarneri, Alessandra A Torrecilhas, Ana C Melo, Maria N Soares, Rodrigo P Mem Inst Oswaldo Cruz Short Communication The protozoan Trypanosoma cruzi has the ability to spontaneously secrete extracellular vesicles (EVs). In this paper, T. cruzi EVs derived from epimastigote forms were evaluated during interaction with triatomine bugs Rhodnius prolixus and Triatoma infestans. T. cruzi EVs were purified and artificially offered to the insects prior to infection with epimastigote forms. No effect of EVs was detected in the parasite counts in the guts of both vectors after 49-50 days. On the other hand, pre-feeding with EVs delayed parasite migration to rectum only in the gut in R. prolixus after 21-22 days. Those data suggest a possible role of T. cruzi EVs during the earlier events of infection in the invertebrate host. Instituto Oswaldo Cruz, Ministério da Saúde 2019-12-13 /pmc/articles/PMC6908325/ /pubmed/31851215 http://dx.doi.org/10.1590/0074-02760190217 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Short Communication
Paranaiba, Larissa F
Guarneri, Alessandra A
Torrecilhas, Ana C
Melo, Maria N
Soares, Rodrigo P
Extracellular vesicles isolated from Trypanosoma cruzi affect early parasite migration in the gut of Rhodnius prolixus but not in Triatoma infestans
title Extracellular vesicles isolated from Trypanosoma cruzi affect early parasite migration in the gut of Rhodnius prolixus but not in Triatoma infestans
title_full Extracellular vesicles isolated from Trypanosoma cruzi affect early parasite migration in the gut of Rhodnius prolixus but not in Triatoma infestans
title_fullStr Extracellular vesicles isolated from Trypanosoma cruzi affect early parasite migration in the gut of Rhodnius prolixus but not in Triatoma infestans
title_full_unstemmed Extracellular vesicles isolated from Trypanosoma cruzi affect early parasite migration in the gut of Rhodnius prolixus but not in Triatoma infestans
title_short Extracellular vesicles isolated from Trypanosoma cruzi affect early parasite migration in the gut of Rhodnius prolixus but not in Triatoma infestans
title_sort extracellular vesicles isolated from trypanosoma cruzi affect early parasite migration in the gut of rhodnius prolixus but not in triatoma infestans
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6908325/
https://www.ncbi.nlm.nih.gov/pubmed/31851215
http://dx.doi.org/10.1590/0074-02760190217
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